Mouse CLEC4F/CLECSF13 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CLECSF4F, rhCLEC4D, rhCLEC4E, recombinant mouse (rm) OCIL, and rmOCIL-rp2 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 370901
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13
    Ala65-Gly548
    Accession # P70194
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CLEC4F/CLECSF13 (Catalog # 2784-CL)
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CLEC4F/CLECSF13 in Mouse Liver.

CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse CLEC4F/CLECSF13 Monoclonal Antibody (Catalog # MAB2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC4F/CLECSF13

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

  • References:
    1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
    2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
    3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
    4. Accession # P70194.
    5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
    6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
    7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
    8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
    9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
    10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
    11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.
  • Long Name:
    C-type Lectin Domain Family 4, Member F
  • Entrez Gene IDs:
    165530 (Human); 51811 (Mouse); 114598 (Rat)
  • Alternate Names:
    CLEC4F; CLECSF13; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13; C-type lectin 13; C-type lectin domain family 4, member F; Galactose Particle Receptor; KCLR; KCR
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