Mouse CRACC/SLAMF7 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB46281P
Detection of CRACC/SLAMF7 in Mouse Splenocytes by Flow Cytometry.
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Mouse CRACC/SLAMF7 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CRACC/SLAMF7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CRACC is observed.
Source
Monoclonal Rat IgG2A Clone # 520914
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CRACC/SLAMF7
Ala22-Gly224
Accession # Q8BHK6
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CRACC/SLAMF7 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CRACC/SLAMF7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rat Anti-Mouse CRACC/SLAMF7 PE‑conjugated Monoclonal Antibody (Catalog # FAB46281P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CRACC/SLAMF7

CRACC (CD2-like Receptor Activating Cytotoxic Cells), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. Mature mouse CRACC consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig‑like V-set domain and one Ig‑like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs. Within the ECD, mouse CRACC shares 53% aa sequence identity with human CRACC. It shares 19%‑35% aa sequence identity with comparable regions of other mouse SLAM proteins including 2B4, BLAME, CD2F-10, CD84, CD229, NTB-A, and SLAM/CD150. Additional isoforms of mouse CRACC are distinguished by deletions and/or substitutions in their cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, Kupffer cells, monocytes, and mature dendritic cells. It interacts homophilically to induce NK, CTL, and B cell activation. In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2.

Entrez Gene IDs
57823 (Human); 75345 (Mouse)
Alternate Names
19A; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD319 antigen; CD319; CRACC; CRACCCD2-like receptor-activating cytotoxic cells; CS119A24 protein; Membrane protein FOAP-12; novel LY9 (lymphocyte antigen 9) like protein; Novel Ly9; Protein 19A; SLAM family member 7; SLAMF7

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