Mouse CX3CL1/Fractalkine Chemokine Domain Antibody
Mouse CX3CL1/Fractalkine Chemokine Domain Antibody Summary
Leu22-Lys105
Accession # O35188
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Mouse CX3CL1/Fractalkine Antibody. Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472). The ND50 is typically 0.3-1.5 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CX3CL1/Fractalkine
CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.
Product Datasheets
Citations for Mouse CX3CL1/Fractalkine Chemokine Domain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Diet-Induced Obesity Promotes Liver Metastasis of Pancreatic Ductal Adenocarcinoma via CX3CL1/CX3CR1 Axis
Authors: Y Sun, XX Zhang, S Huang, H Pan, YZ Gai, YQ Zhou, L Zhu, HZ Nie, DX Li
Journal of Immunology Research, 2022;2022(0):5665964.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Absence of CX3CR1 impairs the internalization of Tau by microglia
Authors: M Bolós, M Llorens-Ma, JR Perea, J Jurado-Arj, A Rábano, F Hernández, J Avila
Mol Neurodegener, 2017;12(1):59.
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PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen
Authors: Bryan Simons
J. Immunol, 2016;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow
Authors: Yoh Goto
Stem Cells, 2016;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Interaction between CX3CL1 and CX3CR1 regulates vasculitis induced by immune complex deposition.
Authors: Morimura S, Sugaya M, Sato S
Am J Pathol, 2013;182(5):1640-7.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010;120(3):907-23.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis.
Authors: Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T
J. Immunol., 2008;181(6):4208-18.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Chemokine receptor CX3CR1 mediates skin wound healing by promoting macrophage and fibroblast accumulation and function.
Authors: Ishida Y, Gao JL, Murphy PM
J. Immunol., 2008;180(1):569-79.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice.
Authors: Ren T, Chen Q, Tian Z, Wei H
Biochem. Biophys. Res. Commun., 2007;364(4):978-84.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.
Authors: Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K
J. Leukoc. Biol., 2007;81(1):176-85.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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