Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL1 in Western blots. Does not cross-react with recombinant human CXCL2, 3, 5, 6, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 11, 12/SDF-1 alpha , 13, 15, recombinant rat (rr) CXCL2, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, rrCXCL5, or recombinant porcine CXCL8.
  • Source
    Monoclonal Rat IgG2A Clone # 124014
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1
    Arg20-Lys96
    Accession # P12850
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CXCL1/GRO alpha /KC/CINC-1 aa 20-96 (Catalog # 453-KC)
    under non-reducing conditions only
  • Neutralization
    Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody. Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL1/KC Monoclonal Antibody (Catalog # MAB4531). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/GRO alpha/KC/CINC-1
CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.
  • Entrez Gene IDs:
    2919 (Human); 14825 (Mouse); 81503 (Rat)
  • Alternate Names:
    CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Applications
Sample Type
  1. Respiratory syncytial virus reverses airway hyperresponsiveness to methacholine in ovalbumin-sensitized mice.
    Authors: Aeffner F, Davis I
    PLoS ONE, 2012;7(10):e46660.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
    Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
    J. Exp. Med., 2012;209(6):1219-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines.
    Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG
    J. Lipid Res., 2011;52(7):1307-18.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011;179(6):2730-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. Innate immune responses to systemic Acinetobacter baumannii infection in mice: neutrophils, but not interleukin-17, mediate host resistance.
    Authors: Breslow JM, Meissler JJ, Hartzell RR
    Infect. Immun., 2011;79(8):3317-27.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice.
    Authors: Traylor ZP, Yu EN, Davis IC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(3):L437-45.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Isotype Controls
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Rat IgG2A Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2A Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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