Mouse CXCL13/BLC/BCA-1 Antibody

Catalog # Availability Size / Price Qty
MAB4701-SP
MAB4701-500
MAB4701-100
Mouse CXCL13/BLC/BCA-1 Antibody in Data
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Product Details
Citations (2)
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Mouse CXCL13/BLC/BCA-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL13/BLC/BCA-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant cotton rat CXCL10, recombinant human CXCL1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, 16, recombinant mouse CXCL1, 2, 4, 6, 7, 9, 10, 11, 12/SDF-1 alpha, 15, 16, recombinant porcine CXCL8, recombinant rat CXCL1, 2, 3/CINC-2 alpha, rr3/CINC-2 beta, or 5 is observed.
Source
Monoclonal Rat IgG2A Clone # 143608
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CXCL13/BLC/BCA-1
Ile22-Ala109
Accession # O55038
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC)
Neutralization
Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 12-30 µg/mL in the presence of 4 µg/mL Recombinant Mouse CXCL13/BLC/BCA‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. View Larger

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA-1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB4701). The ND50is typically 12-30 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References
  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.
Entrez Gene IDs
10563 (Human); 55985 (Mouse)
Alternate Names
ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Product Datasheets

Citations for Mouse CXCL13/BLC/BCA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CXCR5(+) T helper cells mediate protective immunity against tuberculosis.
    Authors: Slight S, Rangel-Moreno J, Gopal R, Lin Y, Fallert Junecko B, Mehra S, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Pavon L, Kaushal D, Reinhart T, Randall T, Khader S
    J Clin Invest, 2013;123(2):712-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines.
    Authors: St John AL, Abraham SN
    Nat. Med., 2009;15(11):1259-65.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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