Mouse CXCL13/BLC/BCA-1 Antibody MAB470-100: R&D Systems

Mouse CXCL13/BLC/BCA-1 Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL13 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL13, rmCXCL11/I‑TAC, rmCXCL9/MIG, or rm CXCL12/SDF‑1 alpha.
  • Source
    Monoclonal Rat IgG2A Clone # 143614
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CXCL13
    Ile22-Ala109
    Accession # O55038
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Mouse CXCL13/BLC/BCA-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CXCL13/BLC/BCA‑1 Antibody (Catalog # MAB470)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody (Catalog # BAF470)
  • ELISA Standard
     
    Recombinant Mouse CXCL13/BLC/BCA-1 Protein (Catalog # 470-BC)
  • Neutralization
    Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 12-30 µg/mL in the presence of 4 µg/mL Recombinant Mouse CXCL13/BLC/BCA‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA‑1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL13/BLC/BCA‑1 Monoclonal Antibody (Catalog # MAB470). The ND50 is typically 12-30 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC) and BCA-1, is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse CXCL13 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse CXCL13 shares 64% amino acid sequence similarity with the human protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized CXCL13 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for CXCL13. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

  • References:
    1. Gunn, M.D. et al. (1998) Nature, 391:799.
    2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
    3. Forster, R. et al. (1996) Cell 87:1037.
  • Entrez Gene IDs:
    10563 (Human); 55985 (Mouse)
  • Alternate Names:
    ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Regulation of T(H)2 development by CXCR5+ dendritic cells and lymphotoxin-expressing B cells.
    Authors: Leon B, Ballesteros-Tato A, Browning JL, Dunn R, Randall TD, Lund FE
    Nat. Immunol., 2102;13(7):681-90.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. CXCL13 antibody for the treatment of autoimmune disorders.
    Authors: Klimatcheva E, Pandina T, Reilly C, Torno S, Bussler H, Scrivens M, Jonason A, Mallow C, Doherty M, Paris M, Smith E, Zauderer M
    BMC Immunol, 2015;16(0):6.
  3. CXCL13 is elevated in Sjogren's syndrome in mice and humans and is implicated in disease pathogenesis.
    Authors: Kramer J, Klimatcheva E, Rothstein T
    J Leukoc Biol, 2013;94(5):1079-89.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage.
    J. Immunol., 2012;189(5):2181-90.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  6. Unique requirements for reactivation of virus-specific memory B lymphocytes.
    Authors: Weisel FJ, Appelt UK, Schneider AM, Horlitz JU, Van Rooijen N, Korner H, Mach M, Winkler TH
    J. Immunol., 2010;185(7):4011-21.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
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Primary Antibodies
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Mouse CXCL13/BLC/BCA-1 Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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