Mouse CXCL13/BLC/BCA-1 Antibody Summary
Accession # O55038
Mouse CXCL13/BLC/BCA-1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA-1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB470). The ND50 is typically 12-30 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL13, also known as B-lymphocyte chemoattractant (BLC) and BCA-1, is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse CXCL13 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse CXCL13 shares 64% amino acid sequence similarity with the human protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized CXCL13 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for CXCL13. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.
- Gunn, M.D. et al. (1998) Nature, 391:799.
- Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
- Forster, R. et al. (1996) Cell 87:1037.
Citations for Mouse CXCL13/BLC/BCA-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Regulation of T(H)2 development by CXCR5+ dendritic cells and lymphotoxin-expressing B cells.
Authors: Leon B, Ballesteros-Tato A, Browning JL, Dunn R, Randall TD, Lund FE
Nat. Immunol., 2102;13(7):681-90.
Sample Types: In Vivo
CXCL13 antibody for the treatment of autoimmune disorders.
Authors: Klimatcheva E, Pandina T, Reilly C, Torno S, Bussler H, Scrivens M, Jonason A, Mallow C, Doherty M, Paris M, Smith E, Zauderer M
BMC Immunol, 2015;16(0):6.
CXCL13 is elevated in Sjogren's syndrome in mice and humans and is implicated in disease pathogenesis.
Authors: Kramer J, Klimatcheva E, Rothstein T
J Leukoc Biol, 2013;94(5):1079-89.
Sample Types: Whole Cells
Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage.
J. Immunol., 2012;189(5):2181-90.
Sample Types: Whole Tissue
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Sample Types: Plasma
Applications: Luminex Development
Unique requirements for reactivation of virus-specific memory B lymphocytes.
Authors: Weisel FJ, Appelt UK, Schneider AM, Horlitz JU, Van Rooijen N, Korner H, Mach M, Winkler TH
J. Immunol., 2010;185(7):4011-21.
Sample Types: In Vivo
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