hh Mouse CXCL13/BLC/BCA-1 Antibody AF470: R&D Systems

Mouse CXCL13/BLC/BCA-1 Antibody

(19 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CXCL13/BLC/BCA‑1
    Ile22-Ala109
    Accession # Q3U1E8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 20-50 µg/mL in the presence of 4 µg/mL Recombinant Mouse CXCL13/BLC/BCA‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin staining. Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA‑1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL13/
BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470). The ND50 is typically 20-50 µg/mL.
Immunohistochemistry
CXCL13/BLC/BCA‑1 in Mouse Intestine. CXCL13/BLC/BCA‑1 was detected in perfusion fixed frozen sections of mouse intestine (Peyer's patch) using Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

  • References:
    1. Gunn, M.D. et al. (1998) Nature, 391:799.
    2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
    3. Forster, R. et al. (1996) Cell 87:1037.
  • Entrez Gene IDs:
    10563 (Human); 55985 (Mouse)
  • Alternate Names:
    ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity
    Authors: T Eddens, W Elsegeiny, ML Garcia-Her, P Castillo, G Trevejo-Nu, K Serody, BT Campfield, SA Khader, K Chen, J Rangel-Mor, JK Kolls
    Cell Rep, 2017;18(13):3078-3090.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia
    Cancer Discov, 2016;6(3):256-69.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Western Blot
  3. Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy
    J Neuroinflammation, 2016;13(1):298.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  4. Stromal cells as trend-setters for cells migrating into the lymph node.
    Authors: Buettner M, Dittrich-Breiholz O, Falk C, Lochner M, Smoczek A, Menzel F, Bornemann M, Bode U
    Mucosal Immunol, 2015;8(3):640-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. CLEC-2 is required for development and maintenance of lymph nodes.
    Authors: Benezech C, Nayar S, Finney B, Withers D, Lowe K, Desanti G, Marriott C, Watson S, Caamano J, Buckley C, Barone F
    Blood, 2014;123(20):3200-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
    Authors: Fleige H, Ravens S, Moschovakis G, Bolter J, Willenzon S, Sutter G, Haussler S, Kalinke U, Prinz I, Forster R
    J Exp Med, 2014;211(4):643-51.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Repetitive hypoxic preconditioning induces an immunosuppressed B cell phenotype during endogenous protection from stroke.
    Authors: Monson N, Ortega S, Ireland S, Meeuwissen A, Chen D, Plautz E, Shubel E, Kong X, Li M, Freriks L, Stowe A
    J Neuroinflammation, 2014;11(0):22.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  8. Anti-CXCL13 antibody can inhibit the formation of gastric lymphoid follicles induced by Helicobacter infection.
    Authors: Yamamoto, K, Nishiumi, S, Yang, L, Klimatcheva, E, Pandina, T, Takahashi, S, Matsui, H, Nakamura, M, Zauderer, M, Yoshida, M, Azuma, T
    Mucosal Immunol, 2014;7(5):1244-54.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  9. Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles.
    Authors: Mionnet C, Mondor I, Jorquera A, Loosveld M, Maurizio J, Arcangeli M, Ruddle N, Nowak J, Aurrand-Lions M, Luche H, Bajenoff M
    PLoS Biol, 2013;11(10):e1001672.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  10. CXCL13 is elevated in Sjogren's syndrome in mice and humans and is implicated in disease pathogenesis.
    Authors: Kramer J, Klimatcheva E, Rothstein T
    J Leukoc Biol, 2013;94(5):1079-89.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. IL-23 is required for long-term control of Mycobacterium tuberculosis and B cell follicle formation in the infected lung.
    Authors: Khader SA, Guglani L, Rangel-Moreno J
    J. Immunol., 2011;187(10):5402-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  12. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  13. The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
    Authors: Suzuki K, Maruya M, Kawamoto S
    Immunity, 2010;33(1):71-83.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  14. Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines.
    Authors: St John AL, Abraham SN
    Nat. Med., 2009;15(11):1259-65.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  15. Role of lymphotoxin-alpha in cigarette smoke-induced inflammation and lymphoid neogenesis.
    Authors: Demoor T, Bracke KR, Maes T, Vandooren B, Elewaut D, Pilette C, Joos GF, Brusselle GG
    Eur. Respir. J., 2009;34(2):405-16.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice.
    Authors: Grabner R, Lotzer K, Dopping S, Hildner M, Radke D, Beer M, Spanbroek R, Lippert B, Reardon CA, Getz GS, Fu YX, Hehlgans T, Mebius RE, van der Wall M, Kruspe D, Englert C, Lovas A, Hu D, Randolph GJ, Weih F, Habenicht AJ
    J. Exp. Med., 2009;206(1):233-48.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  17. Suppression of established experimental autoimmune encephalomyelitis and formation of meningeal lymphoid follicles by lymphotoxin beta receptor-Ig fusion protein.
    Authors: Columba-Cabezas S, Griguoli M, Rosicarelli B, Magliozzi R, Ria F, Serafini B, Aloisi F
    J. Neuroimmunol., 2006;179(1):76-86.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  18. The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
    Authors: Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA
    J. Immunol., 2006;177(10):7296-302.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  19. Regulation of B1 cell migration by signals through Toll-like receptors.
    Authors: Ha SA, Tsuji M, Suzuki K, Meek B, Yasuda N, Kaisho T, Fagarasan S
    J. Exp. Med., 2006;203(11):2541-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
Expand to show all 19 Citations
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