Mouse Dectin-1/CLEC7A Antibody

Catalog # Availability Size / Price Qty
MAB17561
MAB17561-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Mouse Dectin-1/CLEC7A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Dectin‑1/CLEC7A in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dectin-1 is observed and no cross-reactivity with recombinant mouse Dectin-2 or rhDLEC is observed.
Source
Monoclonal Rat IgG2A Clone # 218820
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑1/CLEC7A
Phe69-Leu244
Accession # Q6QLQ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.01 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.05-0.25 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Mouse Dectin-1/CLEC7A (Catalog # 1756-DC) coated at 750 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25%‑34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
  4. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
  5. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. 
  6. Willment, J.A. et al. (2003) J. Immunol. 171:4569. 
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

Product Datasheets

Citations for Mouse Dectin-1/CLEC7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation
    Authors: J Liu, C Wu, X Li, Q Yan, MJT Reaney, Z Jiang
    Carbohydr Polym, 2019;213(0):59-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
    PLoS ONE, 2015;10(3):e0119244.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Detection of a TLR2 agonist by hematopoietic stem and progenitor cells impacts the function of the macrophages they produce.
    Authors: Yanez A, Hassanzadeh-Kiabi N, Ng M, Megias J, Subramanian A, Liu G, Underhill D, Gil M, Goodridge H
    Eur J Immunol, 2013;43(8):2114-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis.
    Authors: Ozment-Skelton TR, deFluiter EA, Ha T, Li C, Graves BM, Ferguson DA, Schweitzer JB, Preizsner J, Brown GD, Gordon S, Kalbfleisch JH, Williams DL
    Crit. Care Med., 2009;37(3):1038-45.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse Dectin-1/CLEC7A Antibody
By Anonymous on 07/11/2016
Application: Func Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse

72 hours cytotoxicity assay with secondary Zap conjugate