Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 218820
View all Dectin-1/CLEC7A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑1/CLEC7A
Phe69-Leu244
Accession # Q6QLQ4

Specificity

Detects mouse Dectin‑1/CLEC7A in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dectin-1 is observed and no cross-reactivity with recombinant mouse Dectin-2 or rhDLEC is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Dectin‑1/CLEC7A Antibody

Detection of Dectin‑1/CLEC7A in Raw264.7 Mouse Cell Line by Flow Cytometry.

Mouse Raw264.7 monocyte/macrophage cell line was stained with Rat Anti-Mouse Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB17561, filled histogram) or Rat IgG2A Isotype Control (MAB006, open histogram) followed by PE-conjugated anti-Rat IgG Secondary Antibody (F0105B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Mouse Dectin‑1/CLEC7A Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.05-0.25 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Mouse Dectin-1/CLEC7A (Catalog # 1756-DC) coated at 750 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Reviewed Applications

Read 2 reviews rated 2.5 using MAB17561 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25%‑34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

References

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
  4. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
  5. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. 
  6. Willment, J.A. et al. (2003) J. Immunol. 171:4569. 
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Alternate Names

CD369, CLEC7A, CLECSF12, Dectin1

Entrez Gene IDs

64581 (Human); 56644 (Mouse)

Gene Symbol

CLEC7A

UniProt

Q6QLQ4

Additional Dectin-1/CLEC7A Products

Product Documents for Mouse Dectin‑1/CLEC7A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Dectin‑1/CLEC7A Antibody

For research use only

Citations for Mouse Dectin‑1/CLEC7A Antibody

Customer Reviews for Mouse Dectin‑1/CLEC7A Antibody (2)

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  • Mouse Dectin-1/CLEC7A Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Mouse
    Verified Customer | Posted 11/01/2021
    Detection of Mouse Dectin-1 on mouse monocytes from PBMCs of C57BL/6 mouse strain. Mouse PBMC were gated on monocytes and Dectin-1 was detected with the Mouse Dectin-1 Antibody (catalog # MAB17561) (BLUE) or Rat IgG2A isotype control (RED), followed by a secondary Goat Anti-Rat IgG APC antibody.
    Mouse Dectin‑1/CLEC7A Antibody MAB17561
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Mouse Dectin-1/CLEC7A Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
    Species: Mouse
    Verified Customer | Posted 07/11/2016
    72 hours cytotoxicity assay with secondary Zap conjugate
    Mouse Dectin‑1/CLEC7A Antibody MAB17561

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