Mouse Dectin-1/CLEC7A Antibody

Name: Mouse Dectin-1/CLEC7A Antibody
Brand: R&D Systems
SKU: MAB17561-SP
Rating: 3 (1 reviews)

MAB17561 4 Citations 1 Review Datasheet / COA / SDS

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MAB17561
MAB17561-SP

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Citations (4)

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Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Dectin-1/CLEC7A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Dectin‑1/CLEC7A in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dectin-1 is observed and no cross-reactivity with recombinant mouse Dectin-2 or rhDLEC is observed.

Source

Monoclonal Rat IgG_2A Clone # 218820

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑1/CLEC7A
Phe69-Leu244
Accession # Q6QLQ4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.01 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

RAW 264.7 mouse monocyte/macrophage cell line

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.05-0.25 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Mouse Dectin-1/CLEC7A (Catalog # 1756-DC) coated at 750 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25%‑34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

References

Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
Taylor, P.R. et al. (2002) J. Immunol. 169:3876.
Willment, J.A. et al. (2003) J. Immunol. 171:4569.
Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
Gantner, B.N. et al. (2005) EMBO J. 24:1277.
Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
Saijo, S. et al. (2007) Nat. Immunol. 8:39.
Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Entrez Gene IDs

64581 (Human); 56644 (Mouse)

Alternate Names

Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

Product Datasheets

Product Datasheet

COA

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Citations for Mouse Dectin-1/CLEC7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation
Authors: J Liu, C Wu, X Li, Q Yan, MJT Reaney, Z Jiang
Carbohydr Polym, 2019;213(0):59-69.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
PLoS ONE, 2015;10(3):e0119244.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Detection of a TLR2 agonist by hematopoietic stem and progenitor cells impacts the function of the macrophages they produce.
Authors: Yanez A, Hassanzadeh-Kiabi N, Ng M, Megias J, Subramanian A, Liu G, Underhill D, Gil M, Goodridge H
Eur J Immunol, 2013;43(8):2114-25.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis.
Authors: Ozment-Skelton TR, deFluiter EA, Ha T, Li C, Graves BM, Ferguson DA, Schweitzer JB, Preizsner J, Brown GD, Gordon S, Kalbfleisch JH, Williams DL
Crit. Care Med., 2009;37(3):1038-45.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry