Mouse Desmocollin-2 Antibody

Catalog # Availability Size / Price Qty
AF7490
AF7490-SP
Desmocollin‑2 in Mouse Embryo.
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Product Details
Citations (2)
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Reviews (1)

Mouse Desmocollin-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Desmocollin-2 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Desmocollin-2 is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Desmocollin-1 and rmDesmocollin-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-2
Arg136-Pro694
Accession # P55292
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Desmocollin-2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Desmocollin‑2 in Mouse Embryo. Desmocollin-2 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Desmocollin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7490) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmocollin-2

DSC-2 (Desmocollin [Greek for "glue-that-binds"]-2; also epithelial type 2 desmocollin) is a 100-120 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of both simple and stratified epithelium that form desmosomes. DSC-2 expression is induced by DSG-2 where it serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. Homodimeric DSC-2 forms in-trans, and DSC-2 may also form heterotypic interactions with DSG-2. A DSC-2:DSG-2 cis-interaction is suggested to block DSG-2 activation of Akt, an action that results in beta -catenin mediated cell proliferation. Mature mouse DSC-2 is a 767 amino acid (aa) type I transmembrane glycoprotein (aa 136-902). The mature molecule contains a 559 aa extracellular region (aa 136-694) with five cadherin domains (aa 136-694) and a 187 aa cytoplasmic tail (aa 716-902). There is one 100-110 kDa splice variant that shows an 11 aa substitution for aa 838-902. Over aa 136-694, mouse DSC-2 shares 87% and 79% aa sequence identity with rat and human DSC-2, respectively.

Entrez Gene IDs
1824 (Human); 13506 (Mouse); 291760 (Rat)
Alternate Names
ARVD11; Cadherin family member 2; CDHF2; CDHF2DGII/III; desmocollin 2; Desmocollin2; Desmocollin-2; Desmocollin-3; Desmosomal glycoprotein II; desmosomal glycoprotein II/III; Desmosomal glycoprotein III; DG2; DSC2; DSC3DKFZp686I11137

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Citations for Mouse Desmocollin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Desmocollin-2 promotes intestinal mucosal repair by controlling integrin-dependent cell adhesion and migration
    Authors: Sven Flemming, Anny-Claude Luissint, Dennis H. M. Kusters, Arturo Raya-Sandino, Shuling Fan, Dennis W. Zhou et al.
    Molecular Biology of the Cell
  2. Alterations in Calcium Handling Are a Common Feature in an Arrhythmogenic Cardiomyopathy Cell Model Triggered by Desmosome Genes Loss
    Authors: M Vallverdú-, D Carreras, GJ Pérez, O Campuzano, R Brugada, M Alcalde
    International Journal of Molecular Sciences, 2023-01-20;24(3):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Mouse Desmocollin-2 Antibody

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Mouse Desmocollin-2 Antibody
By dennis kusters on 05/03/2016
Application: WB Sample Tested: primary mouse epithelium Species: Mouse