Mouse Desmocollin-2 Antibody

R&D Systems | Catalog # AF7490

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Desmocollin-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-2
Arg136-Pro694
Accession # P55292

Specificity

Detects mouse Desmocollin-2 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Desmocollin-2 is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Desmocollin-1 and rmDesmocollin-3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Desmocollin-2 Antibody

Desmocollin-2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Desmocollin‑2 in Mouse Embryo.

Desmocollin-2 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Desmocollin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7490) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Desmocollin-2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse embryo (15 d.p.c.)  

Reviewed Applications

Read 1 review rated 5 using AF7490 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Desmocollin-2

DSC-2 (Desmocollin [Greek for "glue-that-binds"]-2; also epithelial type 2 desmocollin) is a 100-120 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of both simple and stratified epithelium that form desmosomes. DSC-2 expression is induced by DSG-2 where it serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. Homodimeric DSC-2 forms in-trans, and DSC-2 may also form heterotypic interactions with DSG-2. A DSC-2:DSG-2 cis-interaction is suggested to block DSG-2 activation of Akt, an action that results in beta -catenin mediated cell proliferation. Mature mouse DSC-2 is a 767 amino acid (aa) type I transmembrane glycoprotein (aa 136-902). The mature molecule contains a 559 aa extracellular region (aa 136-694) with five cadherin domains (aa 136-694) and a 187 aa cytoplasmic tail (aa 716-902). There is one 100-110 kDa splice variant that shows an 11 aa substitution for aa 838-902. Over aa 136-694, mouse DSC-2 shares 87% and 79% aa sequence identity with rat and human DSC-2, respectively.

Alternate Names

ARVD11, CDHF2, Desmocollin2, DG2, DSC2

Entrez Gene IDs

1824 (Human); 13506 (Mouse); 291760 (Rat)

Gene Symbol

DSC2

UniProt

P55292

Additional Desmocollin-2 Products

Product Documents for Mouse Desmocollin-2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Desmocollin-2 Antibody

For research use only

Related Research Areas

Cadherin Superfamily

Citations for Mouse Desmocollin-2 Antibody

Customer Reviews for Mouse Desmocollin-2 Antibody (1)

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Customer Images

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  • Mouse Desmocollin-2 Antibody
    Name: dennis kusters
    Application: Western Blot
    Sample Tested: primary mouse epithelium
    Species: Mouse
    Verified Customer | Posted 05/03/2016
    Mouse Desmocollin-2 Antibody AF7490

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