Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 259631
View all Dkk-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dkk-1
Ser30-His272
Accession # O54908

Specificity

Detects mouse Dkk-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human Dkk-1, -3, or -4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Dkk‑1 Antibody

Dkk‑1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Dkk‑1 in Mouse Embryo.

Dkk‑1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c) using Mouse Dkk‑1 Monoclonal Antibody (Catalog # MAB1765) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Dkk‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

Western Blot

1 µg/mL
Sample: Recombinant Mouse Dkk‑1 (Catalog # 1765-DK)
Recombinant Mouse Dkk‑1 (Catalog # 5897-DK)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dkk-1

Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Gene Symbol

DKK1

UniProt

O54908

Additional Dkk-1 Products

Product Documents for Mouse Dkk‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Dkk‑1 Antibody

For research use only

Citations for Mouse Dkk‑1 Antibody

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Protocols

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FAQs

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