Mouse DLL4 Antibody

Catalog # Availability Size / Price Qty
AF1389
AF1389-SP
Detection of Mouse DLL4 by Western Blot.
9 Images
Product Details
Citations (63)
FAQs
Supplemental Products
Reviews

Mouse DLL4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human DLL4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DLL4
Ser28-Pro525
Accession # BAB18580
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse DLL4 antibody by Western Blot. View Larger

Detection of Mouse DLL4 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DLL4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry DLL4 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

DLL4 in bEnd.3 Mouse Cell Line. DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry DLL4 antibody in Mouse Embryo by Immunohistochemistry (IHC-P). View Larger

DLL4 in Mouse Embryo. DLL4 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing vasculature. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Detection of Mouse DLL4 by Western Blot View Larger

Detection of Mouse DLL4 by Western Blot MPDZ promotes Notch signaling activity.(A) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Expression level of Notch target genes HEY1, HEY2 and HES1 were analyzed by qPCR 48 hr after transduction. Data are presented as mean ±SD. n ≥ 3; *, p<0.05; **, p<0.01; ***, p<0.001 unpaired Student’s t-test. (B) Cardiac endothelial cells were isolated from Mpdzfl/fl and Mpdz delta EC mice by magnetic beads bound with CD31 antibodies. Expression levels of Notch target genes Hey1 and Hey2 were analyzed by qPCR. Data are presented as mean ±SD. n = 3; *, p<0.05; ***, p<0.001 unpaired Student’s t-test. (C) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Expression levels of DLL1 and DLL4 were analyzed by immunoblotting 48 hr after transduction. beta -actin served as loading control. Data are presented as mean ±SD. n ≥ 3; n.s., not significant. (D) HUVECs were either transduced with adenovirus expressing GFP (ctrl) or with adenovirus expressing MPDZ. Expression levels of DLL1 and DLL4 were analyzed by immunoblotting 48 hr after transduction. beta -actin served as loading control. Data are presented as mean ±SD. n ≥ 3; n.s., not significant. (E) Lung endothelial cells were isolated from Mpdzfl/fl and Mpdz delta EC mice by CD31 magnetic beads. Protein amounts of Dll1 and Dll4 were analyzed by immunoblotting. beta -actin served as loading control. Data are presented as mean ±SD. n = 3; n.s., not significant.10.7554/eLife.32860.007Figure 2—source data 1.Source data of qantitative PCR analysis related to Figure 2A and B.Source data of qantitative PCR analysis related to Figure 2A and B. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29620522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse DLL4 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse DLL4 by Immunocytochemistry/ Immunofluorescence Mpdz does not affect cell cell junction assembly.(A, B) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Cells were cultured under sparse conditions (A) or confluent conditions (B). After PFA fixation cells were stained for DLL1 and Nectin-2 or DLL4 and Nectin-2 and counterstained with DAPI. Images were acquired with the confocal microscope LSM 700. Arrow indicates co-localization of DLL1/4 with Nectin-2 at the cell membrane. Arrow head indicates diminished co-localization at the cell membrane. Scale bar: 10 µm. (C) HUVECs were either transfected with control siRNA (si-ctrl) or with siRNA against Nectin-2 (si-Nectin-2). After PFA fixation cells were stained for DLL1 and Nectin-2 or DLL4 and Nectin-2. Images were acquired with the confocal microscope LSM 700. Arrow indicates localization of DLL1/4 at the cell membrane.Scale bar: 10 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29620522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse DLL4 by Western Blot View Larger

Detection of Mouse DLL4 by Western Blot MPDZ promotes Notch signaling activity.(A) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Expression level of Notch target genes HEY1, HEY2 and HES1 were analyzed by qPCR 48 hr after transduction. Data are presented as mean ±SD. n ≥ 3; *, p<0.05; **, p<0.01; ***, p<0.001 unpaired Student’s t-test. (B) Cardiac endothelial cells were isolated from Mpdzfl/fl and Mpdz delta EC mice by magnetic beads bound with CD31 antibodies. Expression levels of Notch target genes Hey1 and Hey2 were analyzed by qPCR. Data are presented as mean ±SD. n = 3; *, p<0.05; ***, p<0.001 unpaired Student’s t-test. (C) HUVECs were either transduced with lentivirus expressing GFP (sh-ctrl) or with lentivirus expressing shRNA against MPDZ (sh-MPDZ). Expression levels of DLL1 and DLL4 were analyzed by immunoblotting 48 hr after transduction. beta -actin served as loading control. Data are presented as mean ±SD. n ≥ 3; n.s., not significant. (D) HUVECs were either transduced with adenovirus expressing GFP (ctrl) or with adenovirus expressing MPDZ. Expression levels of DLL1 and DLL4 were analyzed by immunoblotting 48 hr after transduction. beta -actin served as loading control. Data are presented as mean ±SD. n ≥ 3; n.s., not significant. (E) Lung endothelial cells were isolated from Mpdzfl/fl and Mpdz delta EC mice by CD31 magnetic beads. Protein amounts of Dll1 and Dll4 were analyzed by immunoblotting. beta -actin served as loading control. Data are presented as mean ±SD. n = 3; n.s., not significant.10.7554/eLife.32860.007Figure 2—source data 1.Source data of qantitative PCR analysis related to Figure 2A and B.Source data of qantitative PCR analysis related to Figure 2A and B. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29620522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse DLL4 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse DLL4 by Immunocytochemistry/ Immunofluorescence Endothelial changes after pericyte depletion. a–f Maximum intensity projection of confocal images from control and DTRiPC P6 retinas stained for IB4 (red) in combination with VEGF-A a, VEGFR2 b, VEGFR3 c, Tie2 d, Esm1 e, and Dll4 f (all in white), as indicated. Note local increase of VEGFR2, VEGFR3, and Esm1 (arrowheads in b, c, e) but not Tie2 or VEGF-A at the edge of the vessel plexus. Dll4 expression in DTRiPC sprouts is increased in some regions (arrowheads) but absent in others (arrows). Scale bar, 100 µm. g–j Quantitation of VEGF-A immunosignals area and intensity g, signal intensity for VEGFR2 h and VEGFR3 i and proportion of Esm1+ area with respect to vascular area j in the P6 control and DTRiPC angiogenic front. Error bars, s.e.m. p-values, Student’s t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29146905), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse DLL4 by Immunohistochemistry View Larger

Detection of Mouse DLL4 by Immunohistochemistry Targeting of Fbxw7 induced the upregulation of Dll4 expression.Anti-Dll4 and Isolectin B4 (IsolB4)-stained retinal whole-mounts of Fbxw7iECKO and littermate control retinas, as indicated (A, B). Arrowheads in (B) mark Dll4+ peripheral sprouts at the edge of the growing plexus, arrows indicate upregulated Dll4 in Fbxw7iECKO retinal capillaries. Quantitative analysis (with Volocity 5; n = 3 for each group) of image data confirmed elevated Dll4 levels (number of pixels) in the mutant endothelium (C). Likewise, quantitative RT-PCR analysis showed reduced Fbxw7 expression but upregulated Dll4 transcript levels in P6 Fbxw7iECKO lungs (D). Expression of the Cdh5 gene was used for normalization. Error bars indicate SEM. P values are indicated as ** (<0.001) and * (p<0.05). Scale bars are 100 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22848434), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse DLL4 by Immunohistochemistry View Larger

Detection of Mouse DLL4 by Immunohistochemistry Targeting of Fbxw7 induced the upregulation of Dll4 expression.Anti-Dll4 and Isolectin B4 (IsolB4)-stained retinal whole-mounts of Fbxw7iECKO and littermate control retinas, as indicated (A, B). Arrowheads in (B) mark Dll4+ peripheral sprouts at the edge of the growing plexus, arrows indicate upregulated Dll4 in Fbxw7iECKO retinal capillaries. Quantitative analysis (with Volocity 5; n = 3 for each group) of image data confirmed elevated Dll4 levels (number of pixels) in the mutant endothelium (C). Likewise, quantitative RT-PCR analysis showed reduced Fbxw7 expression but upregulated Dll4 transcript levels in P6 Fbxw7iECKO lungs (D). Expression of the Cdh5 gene was used for normalization. Error bars indicate SEM. P values are indicated as ** (<0.001) and * (p<0.05). Scale bars are 100 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22848434), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL4

Delta-Like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal Regulated Intramembrane Proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by A Disintegrin Metalloprotease (ADAM) and then by Presenilin/ gamma -Ssecretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

References
  1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
  2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
  3. Walker, L. et al. (2001) Stem Cells 19:543.
  4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
Long Name
Delta-like 4
Entrez Gene IDs
54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names
Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4

Product Datasheets

You must select a language.

x

Citations for Mouse DLL4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

63 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Hypoxia-induced AFAP1L1 regulates pathological neovascularization via the YAP-DLL4-NOTCH axis
    Authors: Ren, JS;Bai, W;Ding, JJ;Ge, HM;Wang, SY;Chen, X;Jiang, Q;
    Journal of translational medicine
  2. Efficacious, safe and stable inhibition of corneal neovascularization by AAV vectored anti-VEGF therapeutics
    Authors: Su W, Sun S, Tian B et al.
    Mol Ther Methods Clin Dev
  3. Host genetic modifiers of nonproductive angiogenesis inhibit breast cancer
    Authors: MJ Flister, SW Tsaih, A Stoddard, C Plasterer, J Jagtap, AK Parchur, G Sharma, AR Prisco, A Lemke, D Murphy, M Al-Gizawiy, M Straza, S Ran, AM Geurts, MR Dwinell, AS Greene, C Bergom, PS LaViolette, A Joshi
    Breast Cancer Res. Treat., 2017-05-31;0(0):.
  4. Heterogeneity in VEGFR3 levels drives lymphatic vessel hyperplasia through cell-autonomous and non-cell-autonomous mechanisms
    Authors: Y Zhang, MH Ulvmar, L Stanczuk, I Martinez-C, M Frye, K Alitalo, T Mäkinen
    Nat Commun, 2018-04-03;9(1):1296.
  5. Targeting FSCN1 with an oral small-molecule inhibitor for treating ocular neovascularization
    Authors: Bai, W;Ren, JS;Xia, M;Zhao, Y;Ding, JJ;Chen, X;Jiang, Q;
    Journal of translational medicine
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/ beta -catenin signaling
    Authors: Biswas, S;Shahriar, S;Bachay, G;Arvanitis, P;Brunken, WJ;Agalliu, D;
    bioRxiv : the preprint server for biology
    Species: Transgenic Mouse, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. PI(4,5)P2-dependent regulation of endothelial tip cell specification contributes to angiogenesis
    Authors: EM Davies, R Gurung, KQ Le, KTT Roan, RP Harvey, GM Mitchell, Q Schwarz, CA Mitchell
    Science Advances, 2023-03-31;9(13):eadd6911.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  8. Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection
    Authors: Q Hou, J Jia, J Lin, L Zhu, S Xie, Q Yu, Y Li
    Cell Reports, 2022-09-27;40(13):111416.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem Cells
    Authors: Choong-kun Lee, Bo-Yoon Park, Taehee Jo, Cheol-Heum Park, Ju-Hee Kim, Kyu-Jin Chung et al.
    Stem Cells Translational Medicine
  10. Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents
    Authors: Raghu Ram Katreddi, Ed Zandro M. Taroc, Sawyer M. Hicks, Jennifer M. Lin, Shuting Liu, Mengqing Xiang et al.
    Development
  11. Gatekeeping role of Nf2/Merlin in vascular tip EC induction through suppression of VEGFR2 internalization
    Authors: JH Bae, MJ Yang, SH Jeong, J Kim, SP Hong, JW Kim, YH Kim, GY Koh
    Science Advances, 2022-06-10;8(23):eabn2611.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. A novel peptide inhibitor of Dll4‐Notch1 signalling and its pro‐angiogenic functions
    Authors: Guofu Zhu, Ying Lin, Tandi Ge, Shekhar Singh, Hao Liu, Linlin Fan et al.
    British Journal of Pharmacology
  13. Induction of osteogenesis by bone-targeted Notch activation
    Authors: C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams
    Elife, 2022-02-04;11(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  14. Vascular Sema3E-Plexin-D1 Signaling Reactivation Promotes Post-stroke Recovery through VEGF Downregulation in Mice
    Authors: Ri Yu, Nam-Suk Kim, Yan Li, Jin-Young Jeong, Sang-Joon Park, Bin Zhou et al.
    Translational Stroke Research
  15. The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition
    Authors: E Azzoni, V Frontera, G Anselmi, C Rode, C James, EM Deltcheva, AS Demian, J Brown, C Barone, A Patelli, JR Harman, M Nicholls, SJ Conway, E Morrissey, SEW Jacobsen, DB Sparrow, AL Harris, T Enver, MFTR de Bruijn
    Cell Reports, 2021-12-14;37(11):110103.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Specialized endothelial tip cells guide neuroretina vascularization and blood-retina-barrier formation
    Authors: Georgia Zarkada, Joel P. Howard, Xue Xiao, Hyojin Park, Mathilde Bizou, Severine Leclerc et al.
    Developmental Cell
  17. ABL001, a Bispecific Antibody Targeting VEGF and DLL4, with Chemotherapy, Synergistically Inhibits Tumor Progression in Xenograft Models
    Authors: DH Yeom, YS Lee, I Ryu, S Lee, B Sung, HB Lee, D Kim, JH Ahn, E Ha, YS Choi, SH Lee, WK You
    International Journal of Molecular Sciences, 2020-12-29;22(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Heritable modifiers of the tumor microenvironment influence nanoparticle uptake, distribution and response to photothermal therapy
    Authors: G Sharma, JM Jagtap, AK Parchur, VR Gogineni, S Ran, C Bergom, SB White, MJ Flister, A Joshi
    Theranostics, 2020-04-06;10(12):5368-5383.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  19. BMP9 signaling promotes the normalization of tumor blood vessels
    Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
    Oncogene, 2020-02-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis
    Authors: R Fu, WC Lv, Y Xu, MY Gong, XJ Chen, N Jiang, Y Xu, QQ Yao, L Di, T Lu, LM Wang, R Mo, ZQ Wu
    Nat Commun, 2020-01-23;11(1):460.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Therapeutic targeting of Notch signaling and immune checkpoint blockade in a spontaneous, genetically-heterogeneous mouse model of T-cell acute lymphoblastic leukemia
    Authors: Jie Gao, Michael Van Meter, Susana Hernandez Hernandez Lopez, Guoying Chen, Ying Huang, Shumei Ren et al.
    Disease Models & Mechanisms
  22. Lysophosphatidic acid-induced YAP/TAZ activation promotes developmental angiogenesis by repressing Notch ligand Dll4
    Authors: D Yasuda, D Kobayashi, N Akahoshi, T Ohto-Nakan, K Yoshioka, Y Takuwa, S Mizuno, S Takahashi, S Ishii
    J. Clin. Invest., 2019-07-23;130(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart
    Authors: S Payne, M Gunadasa-R, A Neal, AN Redpath, J Patel, KM Chouliaras, I Ratnayaka, N Smart, S De Val
    Nat Commun, 2019-07-22;10(1):3276.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development
    Authors: Z Gong, B Shang, Y Chu, X Chen, Q Li, K Liu, Y Chen, Y Huang, Y Han, Q Shang, Z Zheng, L Song, Y Li, R Liu, C Xu, X Zhang, B Liu, L Wang, C Shao, Y Wang, Y Shi
    Cell Death Dis, 2019-06-05;10(6):440.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications
    Authors: M Fernández-, V Casquero-G, W Luo, F Francesca, S Ferreira R, S Del Olmo-C, R Benedito
    Nat Commun, 2019-05-22;10(1):2262.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  26. Venous identity requires BMP signalling through ALK3
    Authors: A Neal, S Nornes, S Payne, MD Wallace, M Fritzsche, P Louphrasit, RN Wilkinson, KM Chouliaras, K Liu, K Plant, R Sholapurka, I Ratnayaka, W Herzog, G Bond, T Chico, G Bou-Ghario, S De Val
    Nat Commun, 2019-01-28;10(1):453.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. The ectodomains determine ligand function in vivo and selectivity of DLL1 and DLL4 toward NOTCH1 and NOTCH2 in vitro
    Authors: Lena Tveriakhina, Karin Schuster-Gossler, Sanchez M Jarrett, Marie B Andrawes, Meike Rohrbach, Stephen C Blacklow et al.
    eLife
  28. MPDZ promotes DLL4-induced Notch signaling during angiogenesis
    Authors: F Tetzlaff, MG Adam, A Feldner, I Moll, A Menuchin, J Rodriguez-, D Sprinzak, A Fischer
    Elife, 2018-04-05;7(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. A novel reporter allele for monitoring Dll4 expression within the embryonic and adult mouse
    Authors: Alexander M. Herman, Alexander M. Rhyner, W. Patrick Devine, Sean P. Marrelli, Benoit G. Bruneau, Joshua D. Wythe
    Biology Open
  30. YAP and TAZ regulate adherens junction dynamics and endothelial cell distribution during vascular development
    Authors: Filipa Neto, Alexandra Klaus-Bergmann, Yu Ting Ong, Silvanus Alt, Anne-Clémence Vion, Anna Szymborska et al.
    eLife
  31. Kaiso differentially regulates components of the Notch signaling pathway in intestinal cells
    Authors: Shaiya C. Robinson, Kristina Klobucar, Christina C. Pierre, Amna Ansari, Svetlana Zhenilo, Egor Prokhortchouk et al.
    Cell Communication and Signaling
  32. The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability
    Authors: AV Shah, GM Birdsey, C Peghaire, ME Pitulescu, NP Dufton, Y Yang, I Weinberg, L Osuna Alma, L Payne, JC Mason, H Gerhardt, RH Adams, AM Randi
    Nat Commun, 2017-07-11;8(0):16002.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  33. O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals
    Authors: Sawaguchi S, Varshney S, Ogawa M et al.
    eLife
  34. Fibroblastic niches prime T cell alloimmunity through Delta-like Notch ligands
    Authors: Jooho Chung, Christen L. Ebens, Eric Perkey, Vedran Radojcic, Ute Koch, Leonardo Scarpellino et al.
    Journal of Clinical Investigation
  35. Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation
    Authors: RB Davis, DO Kechele, ES Blakeney, JB Pawlak, KM Caron
    JCI Insight, 2017-03-23;2(6):e92465.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  36. Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine.
    Authors: Toru Nakata, Hiromichi Shimizu, Sayaka Nagata, Go Ito, Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Reiko Kuno, Sho Anzai, Tatsuro Murano, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe, Ryuichi Okamoto
    Data in Brief, 2016-12-29;0(0):2352-3409.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. Vsx1 Transiently Defines an Early Intermediate V2 Interneuron Precursor Compartment in the Mouse Developing Spinal Cord
    Authors: Cédric Francius, María Hidalgo-Figueroa, Stéphanie Debrulle, Barbara Pelosi, Vincent Rucchin, Kara Ronellenfitch et al.
    Frontiers in Molecular Neuroscience
  38. Angiopoietin receptor Tie2 is required for vein specification and maintenance via regulating COUP-TFII
    Authors: Man Chu, Taotao Li, Bin Shen, Xudong Cao, Haoyu Zhong, Luqing Zhang et al.
    eLife
  39. Blood flow controls bone vascular function and osteogenesis
    Nat Commun, 2016-12-06;7(0):13601.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  40. Notch Signaling Regulates the Homeostasis of Tissue-Restricted Innate-like T Cells
    Authors: Vijaykumar Chennupati
    J Immunol, 2016-06-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  41. Cell autonomous and nonautonomous requirements for Delltalike1 during early mouse retinal neurogenesis
    Authors: Amy N. Riesenberg, Nadean L. Brown
    Developmental Dynamics
  42. Stromal Expression of miR-143/145 Promotes Neoangiogenesis in Lung Cancer Development
    Authors: Nadya Dimitrova, Vasilena Gocheva, Arjun Bhutkar, Rebecca Resnick, Robyn M. Jong, Kathryn M. Miller et al.
    Cancer Discovery
  43. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.
    Authors: Zhou Y, Williams J, Smallwood P, Nathans J
    PLoS ONE, 2015-12-02;10(12):e0143650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  44. Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus
    Authors: Carrie J. Shawber, Lu Lin, Maria Gnarra, Mark V. Sauer, Virginia E. Papaioannou, Jan K. Kitajewski et al.
    Vascular Cell
  45. CD163 interacts with TWEAK to regulate tissue regeneration after ischaemic injury
    Authors: Hirokuni Akahori, Vinit Karmali, Rohini Polavarapu, Alicia N. Lyle, Daiana Weiss, Eric Shin et al.
    Nature Communications
  46. Interleukin-6 Stimulates Defective Angiogenesis.
    Authors: Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F
    Cancer Res, 2015-06-16;75(15):3098-107.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  47. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
    Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
    Nat Med, 2015-04-20;21(5):483-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Specific fibroblastic niches in secondary lymphoid organs orchestrate distinct Notch-regulated immune responses
    Authors: Nicolas Fasnacht, Hsin-Ying Huang, Ute Koch, Stéphanie Favre, Floriane Auderset, Qian Chai et al.
    Journal of Experimental Medicine
  49. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
    Authors: Zou M, Luo H, Xiang M
    Dev Dyn, 2014-09-16;244(1):86-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  50. Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin.
    Authors: Samarelli A, Riccitelli E, Bizzozero L, Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M
    J Biol Chem, 2014-05-23;289(28):19466-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  51. Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine
    Authors: Hiromichi Shimizu, Ryuichi Okamoto, Go Ito, Satoru Fujii, Toru Nakata, Kohei Suzuki et al.
    PeerJ
  52. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014-03-12;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  53. BRG1 promotes COUP-TFII expression and venous specification during embryonic vascular development
    Authors: Reema B. Davis, Carol D. Curtis, Courtney T. Griffin
    Development
  54. Fbxw7 controls angiogenesis by regulating endothelial notch activity.
    Authors: Izumi N, Helker C, Ehling M, Behrens A, Herzog W, Adams RH
    PLoS ONE, 2012-07-27;7(7):e41116.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  55. Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling.
    Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R
    Nature, 2012-03-18;484(7392):110-4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  56. Stalk Cell Phenotype Depends on Integration of Notch and Smad1/5 Signaling Cascades
    Authors: Iván M. Moya, Lieve Umans, Elke Maas, Paulo N. G. Pereira, Karen Beets, Annick Francis et al.
    Developmental Cell
  57. Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system.
    Authors: Stamataki D, Holder M, Hodgetts C
    PLoS ONE, 2011-09-07;6(9):e24484.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  58. Regulation of spinal interneuron development by the Olig-related protein Bhlhb5 and Notch signaling
    Authors: Kaia Skaggs, Donna M. Martin, Bennett G. Novitch
    Development
  59. Pitavastatin-induced angiogenesis and arteriogenesis is mediated by Notch1 in a murine hindlimb ischemia model without induction of VEGF
    Authors: Ryosuke Kikuchi, Kyosuke Takeshita, Yasuhiro Uchida, Megumi Kondo, Xian Wu Cheng, Takayuki Nakayama et al.
    Laboratory Investigation
  60. Vascular endothelial growth factor blockade rapidly elicits alternative proangiogenic pathways in neuroblastoma
    Authors: Nibal Zaghloul, Sonia L. Hernandez, Jae-O Bae, Jianzhong Huang, Jason C Fisher, Alice Lee et al.
    Int J Oncol
  61. Therapeutic efficacy of a DNA vaccine targeting the endothelial tip cell antigen delta-like ligand 4 in mammary carcinoma.
    Authors: Haller BK, Brave A, Wallgard E, Roswall P, Sunkari VG, Mattson U, Hallengard D, Catrina SB, Hellstrom M, Pietras K
    Oncogene, 2010-05-24;29(30):4276-86.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  62. Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation.
    Authors: Tammela T, Zarkada G, Wallgard E, Murtomaki A, Suchting S, Wirzenius M, Waltari M, Hellstrom M, Schomber T, Peltonen R, Freitas C, Duarte A, Isoniemi H, Laakkonen P, Christofori G, Yla-Herttuala S, Shibuya M, Pytowski B, Eichmann A, Betsholtz C, Alitalo K
    Nature, 2008-06-25;454(7204):656-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  63. Abscisic acid: an antiangiogenic phytohormone that modulates the phenotypical plasticity of endothelial cells and macrophages
    Authors: J Chaqour, S Lee, A Ravichandr, B Chaqour
    J. Cell. Sci., 2018-02-02;0(0):.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse DLL4 Antibody

There are currently no reviews for this product. Be the first to review Mouse DLL4 Antibody and earn rewards!

Have you used Mouse DLL4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review