hh Mouse DLL4 Antibody AF1389: R&D Systems

Mouse DLL4 Antibody

(15 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human DLL4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse DLL4
    Ser28-Pro525
    Accession # BAB18580
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse DLL4 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DLL4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry

DLL4 in bEnd.3 Mouse Cell Line. DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DLL4

Delta-Like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal Regulated Intramembrane Proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by A Disintegrin Metalloprotease (ADAM) and then by Presenilin/ gamma -Ssecretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

  • References:
    1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
    2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
    3. Walker, L. et al. (2001) Stem Cells 19:543.
    4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
    5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
    6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
  • Long Name:
    Delta-like 4
  • Entrez Gene IDs:
    54567 (Human); 54485 (Mouse)
  • Alternate Names:
    Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability
    Authors: AV Shah, GM Birdsey, C Peghaire, ME Pitulescu, NP Dufton, Y Yang, I Weinberg, L Osuna Alma, L Payne, JC Mason, H Gerhardt, RH Adams, AM Randi
    Nat Commun, 2017;8(0):16002.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation
    Authors: RB Davis, DO Kechele, ES Blakeney, JB Pawlak, KM Caron
    JCI Insight, 2017;2(6):e92465.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. Notch Signaling Regulates the Homeostasis of Tissue-Restricted Innate-like T Cells
    Authors: Vijaykumar Chennupati
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  4. Blood flow controls bone vascular function and osteogenesis
    Nat Commun, 2016;7(0):13601.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.
    Authors: Zhou Y, Williams J, Smallwood P, Nathans J
    PLoS ONE, 2015;10(12):e0143650.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-Frozen
  6. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
    Authors: Zou M, Luo H, Xiang M
    Dev Dyn, 2015;244(1):86-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. Interleukin-6 Stimulates Defective Angiogenesis.
    Authors: Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F
    Cancer Res, 2015;75(15):3098-107.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
    Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
    Nat Med, 2015;21(5):483-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  9. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin.
    Authors: Samarelli A, Riccitelli E, Bizzozero L, Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M
    J Biol Chem, 2014;289(28):19466-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. Fbxw7 controls angiogenesis by regulating endothelial notch activity.
    Authors: Izumi N, Helker C, Ehling M, Behrens A, Herzog W, Adams RH
    PLoS ONE, 2012;7(7):e41116.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling.
    Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R
    Nature, 2012;484(7392):110-4.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  13. Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system.
    Authors: Stamataki D, Holder M, Hodgetts C
    PLoS ONE, 2011;6(9):e24484.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  14. Therapeutic efficacy of a DNA vaccine targeting the endothelial tip cell antigen delta-like ligand 4 in mammary carcinoma.
    Authors: Haller BK, Brave A, Wallgard E, Roswall P, Sunkari VG, Mattson U, Hallengard D, Catrina SB, Hellstrom M, Pietras K
    Oncogene, 2010;29(30):4276-86.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation.
    Authors: Tammela T, Zarkada G, Wallgard E, Murtomaki A, Suchting S, Wirzenius M, Waltari M, Hellstrom M, Schomber T, Peltonen R, Freitas C, Duarte A, Isoniemi H, Laakkonen P, Christofori G, Yla-Herttuala S, Shibuya M, Pytowski B, Eichmann A, Betsholtz C, Alitalo K
    Nature, 2008;454(7204):656-60.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
Expand to show all 15 Citations
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