Mouse EGF Quantikine ELISA Kit

  (6 citations)     
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Product Details
Assay Procedure
Citations (6)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
  • Sensitivity
    1.64 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant mouse EGF
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse EGF Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse EGF in cell culture supernates, tissue homogenates, serum, plasma, and urine. It contains E. coli-expressed recombinant mouse EGF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse EGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse EGF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 27 28 27
Mean 35.4 78 244 28 65.3 232
Standard Deviation 3.2 7.4 11.8 2.6 4.6 18.4
CV% 9 9.5 4.8 9.3 7 7.9

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 24 25 24
Mean 34.6 86.6 304 34.7 82.8 299
Standard Deviation 3.4 7.9 14.7 3.2 7.7 28.4
CV% 9.8 9.1 4.8 9.2 9.3 9.5

Recovery

The recovery of mouse EGF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 109 101-119
EDTA Plasma (n=4) 97 87-106
Heparin Plasma (n=4) 95 87-105
Serum (n=4) 96 83-107
Tissue Homogenates (n=3) 93 89-101
Linearity
To assess the linearity of the assay, samples spiked with or containing mouse EGF in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 EGF [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: EGF
EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.
  • Long Name:
    Epidermal Growth Factor
  • Entrez Gene IDs:
    1950 (Human); 13645 (Mouse); 25313 (Rat)
  • Alternate Names:
    beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Sample Type
  1. Radioprotective effects of Keratinocyte Growth Factor-1 against irradiation-induced salivary gland hypofunction
    Authors: JS Choi, HS Shin, HY An, YM Kim, JY Lim
    Oncotarget, 2017;8(8):13496-13508.
    Species: Mouse
    Sample Type: Saliva
  2. Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement
    Authors: R Xu, H Xia, W He, Z Li, J Zhao, B Liu, Y Wang, Q Lei, Y Kong, Y Bai, Z Yao, R Yan, H Li, R Zhan, S Yang, G Luo, J Wu
    Sci Rep, 2016;6(0):24596.
    Species: Mouse
    Sample Type: Whole Tissue
  3. Intranasal epidermal growth factor treatment rescues neonatal brain injury.
    Authors: Scafidi J, Hammond T, Scafidi S, Ritter J, Jablonska B, Roncal M, Szigeti-Buck K, Coman D, Huang Y, McCarter R, Hyder F, Horvath T, Gallo V
    Nature, 2014;506(7487):230-4.
    Species: Mouse
    Sample Type: Tissue Homogenates
  4. Mesenchymal stromal cells improve salivary function and reduce lymphocytic infiltrates in mice with Sjogren's-like disease.
    Authors: Khalili S, Liu Y, Kornete M, Roescher N, Kodama S, Peterson A, Piccirillo CA, Tran SD
    PLoS ONE, 2012;7(6):e38615.
    Species: Mouse
    Sample Type: Saliva
  5. Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease.
    Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y
    J. Clin. Invest., 2011;121(11):4548-66.
    Species: Mouse
    Sample Type: Tissue Homogenates
  6. Somatic excision demonstrates that c-Jun induces cellular migration and invasion through induction of stem cell factor.
    Authors: Katiyar S, Jiao X, Wagner E, Lisanti MP, Pestell RG
    Mol. Cell. Biol., 2007;27(4):1356-69.
    Species: Mouse
    Sample Type: Cell Culture Supernates

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