Mouse Endocan/ESM‑1 Biotinylated Antibody

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Product Details
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Mouse Endocan/ESM‑1 Biotinylated Antibody Summary

Species Reactivity
Detects mouse Endocan/ESM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human Endocan is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Endocan/ESM-1 (R&D Systems, Catalog # 1999-EC)
Accession # Q9QYY7
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Endocan/ESM‑1 (Catalog # 1999-EC)

Mouse Endocan/ESM-1 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Endocan/ESM‑1 Antibody (Catalog # MAB1999)

Standard: Recombinant Mouse Endocan Protein, CF (Catalog # 1999-EC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endocan/ESM-1

Endocan (endothelial cell proteoglycan; also known as endothelial-cell specific molecule-1 [ESM-1]), is a 50 kDa, monomeric, secreted, cysteine-rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N-terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N-linked glycosylation sites. Based on human Endocan, there are at least two potential O-linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4-O sulfated N-acetyl galactosamine with alpha -iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan is 96% and 74% aa identical to rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa’s 82 through 131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF-alpha and VEGF, (1, 7) and is known to bind to LFA-1 ( alpha L beta 2) on the surface of PBMCs, blocking LFA-1 interaction with ICAM-1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6).

  1. Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
  2. Lassalle, P. (1999) Genbank Accession #: Q9QYY7.
  3. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
  4. Aitkenhead, M. et al. (2002) Microvasc. Res. 63:159.
  5. Wellner, M. et al. (2003) Horm. Metab. Res. 35:217
  6. Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
  7. Tsai, J.C. et al. (2002) J. Vasc. Res. 39:148.
Entrez Gene IDs
11082 (Human); 71690 (Mouse)
Alternate Names
Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6

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