Mouse EphB3 Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse EphB3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human EphB3 is observed and less than 1% cross-reactivity with recombinant rat EphB1, recombinant mouse (rm) EphA4, rmEphB2, rmEphB4, and rmEphB6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse EphB3
    Leu30-Thr537
    Accession # P54754
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse EphB3 Fc Chimera (Catalog # 432-B3)
  • Flow Cytometry
    2.5 µg/106 cells
    COLO 205 human colorectal adenocarcinoma cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
EphB3 in COLO 205 Human Cell Line. EphB3 was detected in immersion fixed COLO 205 human colorectal adeno­carcinoma cell line using Goat Anti-Mouse EphB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF432) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphB3
EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 525 amino acid (aa) extracellular domain (ECD) of mature mouse EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, mouse EphB3 shares 96% and 99% aa sequence identity with human and rat EphB3, respectively. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6‑9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10‑12). It directs embyronic neuronal axon pathfinding, and its upregulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10‑12, 15).
  • References:
    1. Pasquale, E.B. (2008) Cell 133:38.
    2. Ruiz, J.C. et al. (1994) Mech. Dev. 48:153.
    3. Pasquale, E.B (2004) Nat. Neurosci. 7:417.
    4. Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
    5. Miao, H. et al. (2005) J. Biol. Chem. 280:923.
    6. Adams, R.H. et al. (1999) Genes Dev. 13:295.
    7. Krull, C.E. et al. (1997) Curr. Biol. 7:571.
    8. Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
    9. Cortina, C. et al. (2007) Nature Genet. 39:1376.
    10. Birgbauer, E. et al. (2000) Development 127:1231.
    11. Alfaro, D. et al. (2008) Immunology 125:131.
    12. Risley, M. et al. (2009) Mech. Dev. 126:230.
    13. Liu, X. et al. (2006) J. Neurosci. 26:3087.
    14. Batlle, E. et al. (2005) Nature 435:1126.
    15. Holmberg, J. et al. (2006) Cell 125:1151.
  • Long Name:
    Eph Receptor B3
  • Entrez Gene IDs:
    2049 (Human); 13845 (Mouse)
  • Alternate Names:
    Cek10; EC 2.7.10; EC 2.7.10.1; EK2; EPH receptor B3; EphB3; EPH-like kinase 2; EPH-like tyrosine kinase-2; ephrin type-B receptor 3; ETK2Hek2; Hek2; human embryo kinase 2; Mdk5; Sek4; Tyro6; Tyrosine-protein kinase TYRO6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
    Authors: Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E
    PLoS Genet, 2015;11(11):e1005638.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  2. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
    Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
    Biochem. Biophys. Res. Commun., 2011;405(4):521-6.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism.
    Authors: Bush JO, Soriano P
    Genes Dev., 2009;23(13):1586-99.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
    Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
    J. Cell. Sci., 2006;119(0):559-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
    Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
    J. Biol. Chem., 2006;281(43):32574-86.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. EphB receptors coordinate migration and proliferation in the intestinal stem cell niche.
    Authors: Holmberg J, Genander M, Halford MM, Anneren C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisen J
    Cell, 2006;125(6):1151-63.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
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