Mouse EphB3 Antibody

Detection of Mouse EphB3 by Western Blot.

Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse EphB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF432) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EphB3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EphB3 in COLO 205 Human Cell Line.

EphB3 was detected in immersion fixed COLO 205 human colorectal adeno-carcinoma cell line using Goat Anti-Mouse EphB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF432) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of EphB3 by Immunohistochemistry

WNT4 expression is decreased in the radiation-injured human and mouse intestinal tissues. A HE staining of the small intestines of normal control mouse (Normal) and mouse with 10.5 Gy of abdominal irradiation after 4 weeks (IR). Scale bars, 100 μm. B HE staining of the radiation-injured lesion (L) and paired adjacent non-injured intestinal tissue (N) in RE patient. Scale bars, 100 μm. C IF staining was performed using anti-lysozyme (labeled Paneth cells, red) and anti-EphB3 (green) antibodies in the small intestinal tissues of normal and IR mice. Nuclei were counterstained with DAPI (blue). Scale bars, 100 μm. D ISH analysis of Wnt4 expression in the small intestinal tissues of normal and IR mice. The crypt regions (red boxes) were magnified in the middle column, and the villous regions (black boxes) were magnified in the right column. Scale bars, 200 μm (left column) and 50 μm (middle and right columns). E The intensity of Wnt4 expression in the crypts and villus mesenchyme in the normal (n = 6) and IR (n = 6) mice was quantified using ImageJ software based on the ISH analysis in D. F RT-qPCR analysis of WNT4 expression in seven clinical samples of RE lesions (L) and paired adjacent non-injured intestinal tissues (N). G Correlation analysis between the relative WNT4 mRNA level and EphB3 mRNA level in the intestinal lesions of patients with RE (n = 18). Data are means ± SD. Statistical analysis was performed by unpaired Student’s t-test (E), paired Student’s t-test (F), and Pearson correlation coefficient test (G). ns, not significant, *p < 0.05, **p < 0.01 Image collected and cropped by CiteAb from the following open publication (https://cmbl.biomedcentral.com/articles/10.1186/s11658-024-00677-4), licensed under a CC-BY license. Not internally tested by R&D Systems.