Measured by its ability to neutralize Erythropoietin-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 40 ng/mL Recombinant Mouse Erythropoietin.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Cell Proliferation Induced by Erythropoietin and Neutralization by Mouse Erythropoietin Antibody.
Recombinant Mouse Erythropoietin (Catalog # 959-ME) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Erythropoietin Monoclonal Antibody (Catalog # MAB959). The ND50 is typically 0.2-0.8 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Erythropoietin (Epo) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of Epo is required for biological activities in vivo (4). Mature mouse Epo shares 95% amino acid sequence identity with rat Epo and 73-82% with bovine, canine, equine, feline, human, ovine, and porcine Epo. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R) (8, 9). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of Epo R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). Epo additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).
Koury, M.J. (2005) Exp. Hematol. 33:1263.
Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
Wen, D. et al. (1993) Blood 82:1507.
Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
Eckardt, K.U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
Sharples, E.J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
Acs, G. et al. (2001) Cancer Res. 61:3561.
Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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