Detects mouse FGF-21 in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human (rh) FGF-21 is observed and less than 1% cross-reactivity with rhFGF-19 and rhFGF-23 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse FGF-21 Tyr30-Ser210 Accession # Q9JJN1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse FGF‑21 by Western Blot. Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF‑21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that is
a member of the FGF family. It is synthesized as a 210 amino acid (aa)
precursor that contains a 28 aa signal sequence and a 182 aa mature
region. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequence
identity with human, bovine, canine and rat FGF-21, respectively.
FGF-21 seems to be produced by the liver and exhibits unique
antidiabetogenic effects in fat.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.