Mouse FGF-21 Antibody

Catalog # Availability Size / Price Qty
AF3057
AF3057-SP
Detection of Mouse FGF‑21 by Western Blot.
2 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Mouse FGF-21 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FGF-21 in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human (rh) FGF-21 is observed and less than 1% cross-reactivity with rhFGF-19 and rhFGF-23 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse FGF-21
Tyr30-Ser210
Accession # Q9JJN1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse FGF-21 antibody by Western Blot. View Larger

Detection of Mouse FGF‑21 by Western Blot. Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse FGF-21 by Western Blot View Larger

Detection of Mouse FGF-21 by Western Blot Increased cellular stress response in muscle of DTG mice.(A) Gene expression analysis of FGF21 in Quadriceps muscle by quantitative RT-PCR (n = 8 per group) and (B) FGF21 plasma concentration of 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice (n = 9–10 per group). (B) Representative western blots of proteins involved in integrated stress response and FGF21 induction and (C) of cellular stress response markers HSP25 and HSP70 in Quadriceps muscle from 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice, Mitofusin-2 (MFN2) was used as a loading control (n = 2 out of 6–8 analyzed per group). Means with different letters are significantly different (1way ANOVA and Bonferroni's multiple comparisons test, p<0.05). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0094689), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-21

Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF family. It is synthesized as a 210 amino acid (aa) precursor that contains a 28 aa signal sequence and a 182 aa mature region. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequence identity with human, bovine, canine and rat FGF-21, respectively. FGF-21 seems to be produced by the liver and exhibits unique antidiabetogenic effects in fat.

Long Name
Fibroblast Growth Factor 21
Entrez Gene IDs
26291 (Human); 56636 (Mouse)
Alternate Names
FGF21; FGF-21; fibroblast growth factor 21

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Citations for Mouse FGF-21 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarction
    Authors: JY Sun, LJ Du, XR Shi, YY Zhang, Y Liu, YL Wang, BY Chen, T Liu, H Zhu, Y Liu, CC Ruan, Z Gan, H Ying, Z Yin, PJ Gao, X Yan, RG Li, SZ Duan
    Science Advances, 2023-04-05;9(14):eade4110.
    Species: Mouse
    Sample Types: Cell Lysate
    Applications: Western Blot
  2. SMP30-mediated synthesis of vitamin C activates the liver PPARalpha/FGF21 axis to regulate thermogenesis in mice
    Authors: B Lee, HJ An, DH Kim, MK Lee, HH Jeong, KW Chung, Y Go, AY Seo, IY Kim, JK Seong, BP Yu, J Lee, E Im, IK Lee, MS Lee, KI Yamada, HY Chung
    Experimental & Molecular Medicine, 2022-11-25;0(0):.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. CerS1-Derived C18:0 Ceramide in Skeletal Muscle Promotes Obesity-Induced Insulin Resistance
    Authors: SM Turpin-Nol, P Hammerschm, W Chen, A Jais, K Timper, M Awazawa, S Brodesser, JC Brüning
    Cell Rep, 2019-01-02;26(1):1-10.e7.
    Species: Mouse
    Sample Types: In Vivo, Tissue Homogenates
    Applications: Neutralization, Western Blot
  4. Chronic Over-expression of Fibroblast Growth Factor 21 Increases Bile Acid Biosynthesis by Opposing FGF15/19 Action
    EBioMedicine, 2016-12-24;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  5. Activation of AMPKalpha2 is not crucial for mitochondrial uncoupling-induced metabolic effects but required to maintain skeletal muscle integrity.
    Authors: Ost M, Werner F, Dokas J, Klaus S, Voigt A
    PLoS ONE, 2014-04-14;9(4):e94689.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Silencing of the Fibroblast growth factor 21 gene is an underlying cause of acinar cell injury in mice lacking MIST1.
    Authors: Johnson, Charis L, Mehmood, Rashid, Laing, Scott W, Stepniak, Camilla, Kharitonenkov, Alexei, Pin, Christop
    Am J Physiol Endocrinol Metab, 2014-02-18;306(8):E916-28.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Activation of the farnesoid x receptor induces hepatic expression and secretion of fibroblast growth factor 21.
    Authors: Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB
    J. Biol. Chem., 2012-06-01;287(30):25123-38.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. BetaKlotho is required for metabolic activity of fibroblast growth factor 21.
    Authors: Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M
    Proc. Natl. Acad. Sci. U.S.A., 2007-04-23;104(18):7432-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

Reviews for Mouse FGF-21 Antibody

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Mouse FGF-21 Antibody
By Hua Jiang on 11/01/2016
Application: WB Sample Tested: Liver tissue,Spleen tissue Species: Human, Mouse, Rat

I didn't see the band in mouse spleen tissue.