Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Neutralization, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FGF-21 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse FGF-21
Tyr30-Ser210
Accession # Q9JJN1

Specificity

Detects mouse FGF-21 in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human (rh) FGF-21 is observed and less than 1% cross-reactivity with rhFGF-19 and rhFGF-23 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse FGF‑21 Antibody

Detection of Mouse FGF-21 antibody by Western Blot.

Detection of Mouse FGF‑21 by Western Blot.

Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse FGF-21 by Western Blot

Detection of Mouse FGF-21 by Western Blot

Increased cellular stress response in muscle of DTG mice.(A) Gene expression analysis of FGF21 in Quadriceps muscle by quantitative RT-PCR (n = 8 per group) and (B) FGF21 plasma concentration of 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice (n = 9–10 per group). (B) Representative western blots of proteins involved in integrated stress response and FGF21 induction and (C) of cellular stress response markers HSP25 and HSP70 in Quadriceps muscle from 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice, Mitofusin-2 (MFN2) was used as a loading control (n = 2 out of 6–8 analyzed per group). Means with different letters are significantly different (1way ANOVA and Bonferroni's multiple comparisons test, p<0.05). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0094689), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse FGF-21 Antibody by Western Blot

Detection of Mouse Mouse FGF-21 Antibody by Western Blot

Increased cellular stress response in muscle of DTG mice.(A) Gene expression analysis of FGF21 in Quadriceps muscle by quantitative RT-PCR (n = 8 per group) and (B) FGF21 plasma concentration of 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice (n = 9–10 per group). (B) Representative western blots of proteins involved in integrated stress response and FGF21 induction and (C) of cellular stress response markers HSP25 and HSP70 in Quadriceps muscle from 12-wk-old WT, DN-AMPK alpha 2, UCP1-TG and DTG mice, Mitofusin-2 (MFN2) was used as a loading control (n = 2 out of 6–8 analyzed per group). Means with different letters are significantly different (1way ANOVA and Bonferroni's multiple comparisons test, p<0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24732703), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FGF-21 by Western Blot

Detection of FGF-21 by Western Blot

FGF21 links SEL1L-HRD1 ERAD in skeletal muscle to systemic metabolic regulation.(A) Representative Western blot of ER homeostasis proteins and FGF21 in WT, Sel1LMLC (SKO), FGF21MLC (FKO), and Sel1L/Fgf21 double knockout (DKO) mice (n = 4 mice per genotype). (B) Serum measurement of FGF21 from male and female mice at 16–20 weeks old (n = 5–7 per genotype). (C) Body length of 16-week-old mice (n = 7–20 for males, n = 7–14 for females). (D) Weekly body mass of male and female mice (n = 6–44 per genotype/time point for males, n = 5–45 per genotype/time point for females). Statistical comparison made between Sel1LMLC and DKO mice. (E) Muscle mass–to–body mass ratios in male mice (n = 4–18 per genotype). Comparisons were made between indicated groups. FKO muscle was not statistically different from WT. (F) Representative H&E-stained images of inguinal white adipose tissue (n = 3 mice per genotype). (G) Representative Western blot of UCP1 in WT, Sel1LMLC, FGF21MLC, and DKO mice (n = 4 mice per genotype). Data presented as mean ± SEM. NS, P > 0.05; *P < 0.05; **P < 0.01; ****P < 0.0001 determined by 1-way ANOVA with Tukey’s multiple-comparison test (B, C, and E) or mixed-effects analysis (repeated-measure ANOVA) with Tukey’s multiple-comparison test (D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37535424), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of FGF-21 by Western Blot

Detection of FGF-21 by Western Blot

FGF21 links SEL1L-HRD1 ERAD in skeletal muscle to systemic metabolic regulation.(A) Representative Western blot of ER homeostasis proteins and FGF21 in WT, Sel1LMLC (SKO), FGF21MLC (FKO), and Sel1L/Fgf21 double knockout (DKO) mice (n = 4 mice per genotype). (B) Serum measurement of FGF21 from male and female mice at 16–20 weeks old (n = 5–7 per genotype). (C) Body length of 16-week-old mice (n = 7–20 for males, n = 7–14 for females). (D) Weekly body mass of male and female mice (n = 6–44 per genotype/time point for males, n = 5–45 per genotype/time point for females). Statistical comparison made between Sel1LMLC and DKO mice. (E) Muscle mass–to–body mass ratios in male mice (n = 4–18 per genotype). Comparisons were made between indicated groups. FKO muscle was not statistically different from WT. (F) Representative H&E-stained images of inguinal white adipose tissue (n = 3 mice per genotype). (G) Representative Western blot of UCP1 in WT, Sel1LMLC, FGF21MLC, and DKO mice (n = 4 mice per genotype). Data presented as mean ± SEM. NS, P > 0.05; *P < 0.05; **P < 0.01; ****P < 0.0001 determined by 1-way ANOVA with Tukey’s multiple-comparison test (B, C, and E) or mixed-effects analysis (repeated-measure ANOVA) with Tukey’s multiple-comparison test (D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37535424), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse FGF‑21 Antibody

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: Mouse spleen tissue and rat spleen tissue

Reviewed Applications

Read 1 review rated 4 using AF3057 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-21

Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF family. It is synthesized as a 210 amino acid (aa) precursor that contains a 28 aa signal sequence and a 182 aa mature region. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequence identity with human, bovine, canine and rat FGF-21, respectively. FGF-21 seems to be produced by the liver and exhibits unique antidiabetogenic effects in fat.

Long Name

Fibroblast Growth Factor 21

Alternate Names

FGF21

Entrez Gene IDs

26291 (Human); 56636 (Mouse)

Gene Symbol

FGF21

UniProt

Q9JJN1

Additional FGF-21 Products

Product Documents for Mouse FGF‑21 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse FGF‑21 Antibody

For research use only

Citations for Mouse FGF‑21 Antibody

Customer Reviews for Mouse FGF‑21 Antibody (1)

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  • Mouse FGF-21 Antibody
    Name: Hua Jiang
    Application: Western Blot
    Sample Tested: Liver tissue and Spleen tissue
    Species: Human, Rat and Mouse
    Verified Customer | Posted 11/01/2016
    I didn't see the band in mouse spleen tissue.
    Mouse FGF‑21 Antibody AF3057

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