Mouse FGF-21 Antibody

(5 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse FGF-21 in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human (rh) FGF-21 is observed and less than 1% cross-reactivity with rhFGF-19 and rhFGF-23 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse FGF-21
    Tyr30-Ser210
    Accession # Q9JJN1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse FGF‑21 by Western Blot. Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF‑21 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF‑21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-21
Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF family. It is synthesized as a 210 amino acid (aa) precursor that contains a 28 aa signal sequence and a 182 aa mature region. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequence identity with human, bovine, canine and rat FGF-21, respectively. FGF-21 seems to be produced by the liver and exhibits unique antidiabetogenic effects in fat.
  • Long Name:
    Fibroblast Growth Factor 21
  • Entrez Gene IDs:
    26291 (Human); 56636 (Mouse)
  • Alternate Names:
    FGF21; FGF-21; fibroblast growth factor 21
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Chronic Over-expression of Fibroblast Growth Factor 21 Increases Bile Acid Biosynthesis by Opposing FGF15/19 Action
    EBioMedicine, 2016;0(0):.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Capture
  2. Activation of AMPKalpha2 is not crucial for mitochondrial uncoupling-induced metabolic effects but required to maintain skeletal muscle integrity.
    Authors: Ost M, Werner F, Dokas J, Klaus S, Voigt A
    PLoS ONE, 2014;9(4):e94689.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Silencing of the Fibroblast growth factor 21 gene is an underlying cause of acinar cell injury in mice lacking MIST1.
    Authors: Johnson, Charis L, Mehmood, Rashid, Laing, Scott W, Stepniak, Camilla, Kharitonenkov, Alexei, Pin, Christop
    Am J Physiol Endocrinol Metab, 2014;306(8):E916-28.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  4. Activation of the farnesoid x receptor induces hepatic expression and secretion of fibroblast growth factor 21.
    Authors: Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB
    J. Biol. Chem., 2012;287(30):25123-38.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  5. BetaKlotho is required for metabolic activity of fibroblast growth factor 21.
    Authors: Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(18):7432-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 application: Western Blot.
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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse FGF-21 Antibody AF3057
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Very Good
 WB Anonymous 11/01/2016
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Western Blot Mouse FGF-21 Antibody AF3057
Western Blot: Mouse FGF-21 Antibody [AF3057]

Summary

ApplicationWestern Blot
Sample TestedLiver tissue,Spleen tissue

Other Experimental Details

Other Experimental DetailsI didn't see the band in mouse spleen tissue.

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