Mouse FGF acidic Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse FGF acidic in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human FGF acidic is observed, and less than 1% cross-reactivity with recombinant mouse (rm) FGF-7, rmFGF-21, and rmFGF-23 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse FGF acidic
    Phe16-Asp155
    Accession # NP_034327
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize FGF acidic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.The Neutralization Dose (ND50) is typically 0.1-0.6 ug/mL in the presence of 1 ng/mL Recombinant Mouse FGF acidic.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse FGF acidic by Western Blot. Western blot shows lysates of mouse heart tissue and mouse kidney tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse FGF acidic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4686) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FGF acidic at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cell Proliferation Induced by FGF acidic and Neutralization by Mouse FGF acidic Antibody. Recombinant Mouse FGF acidic (Catalog # 4686-FA) induces proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF acidic (1 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse FGF acidic Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF4686). The ND50 is typically 0.1-0.6 ug/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF acidic

FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Mouse FGF acidic shares 52% amino acid (aa) sequence identity with FGF basic and 15%-35% with other mouse FGFs. It shares 91%, 96%, 94%, and 100% aa sequence identity with bovine, human, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (4). The secreted FGF acidic is stored in complex with extracellular heparan sulfate (5). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulating FGF acidic bioactivity (6). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (7, 8). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (9). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (10-12). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (13, 14). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (15).

  • References:
    1. Madiai, F. et al. (1996) Gene 179:231.
    2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
    3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
    4. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
    5. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
    6. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
    7. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
    8. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
    9. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
    10. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
    11. Imamura, T. et al. (1990) Science 249:1567.
    12. Wesche, J. et al. (2005) Biochemistry 44:6071.
    13. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
    14. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
    15. Bouleau, S. et al. (2005) Oncogene 24:7839.
  • Long Name:
    Fibroblast Growth Factor acidic
  • Entrez Gene IDs:
    2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
  • Alternate Names:
    AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF1; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1
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