Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF R4
Leu19-Asp366
Accession # CAA42551
Leu19-Asp366
Accession # CAA42551
Specificity
Detects mouse FGF R4 in Western blots. In Western blots, approximately 35% cross‑reactivity with recombinant human (rh) FGF R4 is observed and less than 5% cross-reactivity with rhFGF R1 alpha (IIIb), rhFGF R1 alpha (IIIc), rhFGF R1 beta (IIIb), rhFGF R1 beta (IIIc), recombinant mouse (rm) FGF R2 alpha (IIIb), rmFGF R2 alpha (IIIc), rmFGF R2 beta (IIIb), rmFGF R2 beta (IIIc), rmFGF R5, and rmFGF R5 beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse FGFR4 Biotinylated Antibody
FGF R4 in Mouse Embryo.
FGF R4 was detected in immersion fixed frozen sections of mouse embryo (E11.5) using Goat Anti-Mouse FGF R4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2265) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse FGFR4 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E11.5)
Sample: Immersion fixed frozen sections of mouse embryo (E11.5)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse FGF R4
Sample: Recombinant Mouse FGF R4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FGFR4
References
- Umbhauer, M.J. et al. (2000) J. Cell Sci. 113:2865.
- Cai, J. (2002) Dev. Biol. 251:221.
Long Name
Fibroblast Growth Factor Receptor 4
Alternate Names
CD334, FGF R4
Gene Symbol
FGFR4
UniProt
Additional FGFR4 Products
Product Documents for Mouse FGFR4 Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse FGFR4 Biotinylated Antibody
For research use only
Related Research Areas
Customer Reviews for Mouse FGFR4 Biotinylated Antibody
There are currently no reviews for this product. Be the first to review Mouse FGFR4 Biotinylated Antibody and earn rewards!
Have you used Mouse FGFR4 Biotinylated Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
