Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all FGFR4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FGF R4
Leu19-Asp366
Accession # CAA42551

Specificity

Detects mouse FGF R4 in Western blots. In Western blots, approximately 35% cross‑reactivity with recombinant human (rh) FGF R4 is observed and less than 5% cross-reactivity with rhFGF R1 alpha  (IIIb), rhFGF R1 alpha  (IIIc), rhFGF R1 beta  (IIIb), rhFGF R1 beta  (IIIc), recombinant mouse (rm) FGF R2 alpha  (IIIb), rmFGF R2 alpha  (IIIc), rmFGF R2 beta  (IIIb), rmFGF R2 beta  (IIIc), rmFGF R5, and rmFGF R5 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse FGFR4 Biotinylated Antibody

FGF R4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

FGF R4 in Mouse Embryo.

FGF R4 was detected in immersion fixed frozen sections of mouse embryo (E11.5) using Goat Anti-Mouse FGF R4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2265) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse FGFR4 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E11.5)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse FGF R4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR4

FGF R4 is a transmembrane receptor tyrosine kinase that binds several ligands of the FGF family. It is expressed in the early embryo, and its expression has been suggested to play an important role in the formation of mesoderm and posterior structure (1). Recent studies also indicated that FGF R4 may be used as a marker to distinguish neural stem cells from other precursor cells (2). The extracellular domain of mouse FGF R4 shares 95% and 89% amino acid sequence homology with its rat and human homologs, respectively.

References

  1. Umbhauer, M.J. et al. (2000) J. Cell Sci. 113:2865.
  2. Cai, J. (2002) Dev. Biol. 251:221.

Long Name

Fibroblast Growth Factor Receptor 4

Alternate Names

CD334, FGF R4

Entrez Gene IDs

2264 (Human); 14186 (Mouse)

Gene Symbol

FGFR4

UniProt

CAA42551

Additional FGFR4 Products

Product Documents for Mouse FGFR4 Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse FGFR4 Biotinylated Antibody

For research use only

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Protocols

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