Mouse FoxN1 Antibody

Catalog # Availability Size / Price Qty
MAB7295
MAB7295-SP
FoxN1 in Mouse Liver.
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Mouse FoxN1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FoxN1 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 717008
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse FoxN1
Val2-Asp164
Accession # Q61575
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FoxN1 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

FoxN1 in Mouse Liver. FoxN1 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse FoxN1 Monoclonal Antibody (Catalog # MAB7295) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxN1

Forkhead box N1 (FoxN1) is a 69 kDa transcriptional regulator that is primarily expressed in thymic epithelial cells and keratinocytes. FoxN1 function is required for proper T cell, hair follicle, and nail differentiation. Mutations in FoxN1 result in severe combined immunodeficiency (SCID/Nude). Mouse FoxN1 contains one DNA-binding forkhead domain (aa 271-367). Within amino acids 1 - 164, mouse FoxN1 shares 84% and 95% aa sequence identity with human and rat FoxN1, respectively.

Long Name
Forkhead Box N1
Entrez Gene IDs
8456 (Human); 15218 (Mouse); 287469 (Rat)
Alternate Names
FKHL20; forkhead box N1; forkhead box protein N1; FoxN1; RONU; Rowett nude; WHN; WHNRONU; winged helix nude; winged-helix nude; Winged-helix transcription factor nude

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Mouse FoxN1 Antibody
By Anonymous on 12/29/2021
Application: IHC Sample Tested: Liver tissue Species: Mouse