Mouse Frizzled-1 Antibody (162531) [Biotin]

R&D Systems | Catalog # BAM11201

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 162531
View all Frizzled-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln1-His248 (Met122Ile)
Accession # O70421

Specificity

Detects mouse Frizzled-1 in direct ELISAs and Western blots and detects human Frizzled-1 by flow cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse Frizzled-1 Antibody (162531) [Biotin]

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: NS0 cell line transfected with mouse Frizzled-1HEK293 human embryonic kidney cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse Frizzled-1 Fc Chimera (Catalog # 1120-FZ)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Frizzled-1

Frizzled-1 belongs to the frizzled family of 7-transmembrane domain proteins that are receptors for Wnt proteins. Frizzled-1 is expressed in a wide variety of tissues and is involved in cell differentiation, embryonal development and tumorigenesis.

Alternate Names

Frizzled1, FZD1

Entrez Gene IDs

8321 (Human); 14362 (Mouse)

Gene Symbol

FZD1

UniProt

O70421

Additional Frizzled-1 Products

Product Documents for Mouse Frizzled-1 Antibody (162531) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Frizzled-1 Antibody (162531) [Biotin]

For research use only

Related Research Areas

Wnt Family
Wnt Receptors

Citations for Mouse Frizzled-1 Antibody (162531) [Biotin]

Customer Reviews for Mouse Frizzled-1 Antibody (162531) [Biotin]

There are currently no reviews for this product. Be the first to review Mouse Frizzled-1 Antibody (162531) [Biotin] and earn rewards!

Have you used Mouse Frizzled-1 Antibody (162531) [Biotin]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies