Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 162531
View all Frizzled-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln1-His248 (Met122Ile)
Accession # O70421

Specificity

Detects mouse Frizzled-1 in direct ELISAs and Western blots and detects human Frizzled-1 by flow cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse Frizzled‑1 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: NS0 cell line transfected with mouse Frizzled-1HEK293 human embryonic kidney cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse Frizzled-1 Fc Chimera (Catalog # 1120-FZ)

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Frizzled-1

Frizzled-1 belongs to the frizzled family of 7-transmembrane domain proteins that are receptors for Wnt proteins. Frizzled-1 is expressed in a wide variety of tissues and is involved in cell differentiation, embryonal development and tumorigenesis.

Alternate Names

Frizzled1, FZD1

Entrez Gene IDs

8321 (Human); 14362 (Mouse)

Gene Symbol

FZD1

UniProt

O70421

Additional Frizzled-1 Products

Product Documents for Mouse Frizzled‑1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Frizzled‑1 Biotinylated Antibody

For research use only

Related Research Areas

Wnt Family
Wnt Receptors

Citations for Mouse Frizzled‑1 Biotinylated Antibody

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FAQs

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