Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 291004
View all Frizzled-9 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Frizzled‑9
Ala23-Asp186 (predicted)
Accession # Q9R216

Specificity

Detects mouse Frizzled-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3, -4, -6, -7, -8, rmFRP, or recombinant human Frizzled-5 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Frizzled‑9 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Frizzled‑9 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Frizzled-9

Frizzled-9 belongs to the Frizzled family of seven-transmembrane receptors, which are signaling receptors for the family of secreted Wnt ligands. The amino-terminal extracellular cysteine-rich domains of human and mouse Frizzled-9 share 94% amino acid sequence homology.

Alternate Names

CD349, Frizzled9, FZD9

Entrez Gene IDs

8326 (Human); 14371 (Mouse)

Gene Symbol

FZD9

UniProt

Q9R216

Additional Frizzled-9 Products

Product Documents for Mouse Frizzled‑9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Frizzled‑9 Antibody

For research use only

Related Research Areas

Wnt Family
Wnt Receptors

Citations for Mouse Frizzled‑9 Antibody

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