Galectin-2 (Gal-2; also LEG2, HL14 and L-14-II) is a 14-15 kDa member of the S-type (1-CRD type) lectin family of proteins. It is a potentially secreted, noncovalent homodimer that is expressed by stomach mucosal columnar epithelium, small intestine Goblet cells and keratinocytes. Galectin-2 is reported to bind to CD29, and initiate CD8+ T cell apoptosis. Structurally, it serves to bind nonsialylated poly-NAcetyllactosamine motifs. Mouse Galectin-2 is 130 amino acids (aa) in length. There is one galectin domain (aa 4-130) that contains one dimerization interface (aa 123-128). Full-length mouse Galectin-2 shares 86% and 66% aa identity with rat and human Galectin-2, respectively.
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 747307
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Galectin-2
Ser2-Glu130
Accession # Q9CQW5
Ser2-Glu130
Accession # Q9CQW5
Specificity
Detects mouse Galectin-2 in direct ELISAs and Western blots. In Western blots, approximately 5%
cross-reactivity with recombinant human (rh) Galectin-2 is observed and no cross-reactivity
with rhGalectin-4, -8, -10, recombinant mouse Galectin-1, -3, -7, or -9 is
observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Galectin‑2 Antibody
Detection of Mouse Galectin‑2 by Western Blot.
Western blot shows lysates of mouse stomach tissue. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Mouse Galectin-2 Monoclonal Antibody (Catalog # MAB6667) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin-2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin‑2 in Mouse Stomach.
Galectin-2 was detected in perfusion fixed frozen sections of mouse stomach using Rat Anti-Mouse Galectin-2 Monoclonal Antibody (Catalog # MAB6667) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to gastric pits. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Applications for Mouse Galectin‑2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse stomach
Sample: Perfusion fixed frozen sections of mouse stomach
Western Blot
0.2 µg/mL
Sample: Mouse stomach tissue
Sample: Mouse stomach tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Galectin-2
Alternate Names
GAL2, Galectin2, LGALS2
Gene Symbol
LGALS2
UniProt
Additional Galectin-2 Products
Product Documents for Mouse Galectin‑2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Galectin‑2 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars