Detection of Mouse Galectin‑2 by Western Blot.
Western blot shows lysates of mouse stomach tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Galectin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Galectin‑2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin‑2 in Mouse Intestine.
Galectin‑2 was detected in perfusion fixed frozen sections of mouse intestine using Mouse Galectin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Galectin-2 (Gal-2; also LEG2, HL14 and L-14-II) is a 14-15 kDa member of the S-type (1-CRD type) lectin family of proteins. It is a potentially secreted, noncovalent homodimer that is expressed by stomach mucosal columnar epithelium, small intestine Goblet cells and keratinocytes. Galectin-2 is reported to bind to CD29, and initiate CD8+ T cell apoptosis. Structurally, it serves to bind nonsialylated poly-NAcetyllactosamine motifs. Mouse Galectin-2 is 130 amino acids (aa) in length. There is one galectin domain (aa 4-130) that contains one dimerization interface (aa 123-128). Full-length mouse Galectin-2 shares 86% and 66% aa identity with rat and human Galectin-2, respectively.
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