Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Galectin-9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Galectin‑9
Ala2-Thr353; aa 148-178 deletion
Accession # O08573

Specificity

Detects mouse Galectin‑9 in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) Galectin-9 is observed. In direct ELISAs and Western blots, less than 2% cross‑reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-3, rhGalectin-7, rhGalectin-8, recombinant mouse (rm) Galectin-1, rmGalectin-3, rmGalectin-4, and rmGalectin-7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Galectin‑9 Antibody

Detection of Galectin-9 antibody in Mouse Thymocytes antibody by Flow Cytometry.

Detection of Galectin-9 in Mouse Thymocytes by Flow Cytometry.

Mouse thymocytes were stained with Goat Anti-Mouse Galectin-9 Short Isoform Affinity-purified Polyclonal Antibody (Catalog # AF3535, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Galectin-9 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

Galectin‑9 in Mouse Splenocytes.

Galectin-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3535) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse Galectin‑9 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Mouse thymocytes fixed with paraformaldehyde and permeailized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Galectin‑9

Reviewed Applications

Read 5 reviews rated 3.4 using AF3535 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-9

Galectin-9, also known as Ecalectin, belongs to the Galectin family (S-type lectins) of galactoside-binding proteins. It is a 36‑39 kDa protein that lacks a secretion signal sequence, but can be secreted by a non-classical pathway. Galectin-9 is a tandem-repeat Galectin with two Galectin domains joined by a linker region. Two alternative spliced variants, a short and a long isoform, exist. Galectin-9 short isoform is expressed in a variety of tissues and cells, including fibroblasts, hepatocytes, endothelial cells and astrocytes. The long isoform, however, is expressed primarily in the intestine. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 shares 69% and 85% amino acid sequence identity with human and rat Galectin-9, respectively.

Alternate Names

Ecalectin, GAL9, Galectin9, LGALS9

Entrez Gene IDs

3965 (Human); 16859 (Mouse)

Gene Symbol

LGALS9

UniProt

O08573

Additional Galectin-9 Products

Product Documents for Mouse Galectin‑9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Galectin‑9 Antibody

For research use only

Citations for Mouse Galectin‑9 Antibody

Customer Reviews for Mouse Galectin‑9 Antibody (5)

3.4 out of 5
5 Customer Ratings
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Showing  1 - 5 of 5 reviews Showing All
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  • Mouse Galectin-9 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 03/08/2022
    Mouse Galectin‑9 Antibody AF3535
  • Mouse Galectin-9 Antibody
    Name: Shubhankar Ambike
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 02/24/2020
    Mouse alveolar macrophage- MH-S cell line. Total proteins were extracted from the cells and analysed for gal-9 expression by western blot. A lot of bands were seen and it was very difficult to determine which bands are the specific ones. According to the manual, bands boxed in a red rectangle are supposed to be the specific bands. Overall, the experiment was not at all conclusive. No bands were seen in supernatent as well. Not recommended to use for native expression detection in mouse macrophage cells.
    Mouse Galectin‑9 Antibody AF3535
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23836896
    Species: Mouse
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 19851072
    Species: Mouse
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 19670381
    Species: Mouse
    Verified Customer | Posted 01/07/2015

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Protocols

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FAQs

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