Mouse Galectin-9 Antibody

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Detection of Galectin-9 in Mouse Thymocytes by Flow Cytometry.
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Product Details
Citations (8)
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Reviews (2)

Mouse Galectin-9 Antibody Summary

Species Reactivity
Detects mouse Galectin‑9 in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) Galectin-9 is observed. In direct ELISAs and Western blots, less than 2% cross‑reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-3, rhGalectin-7, rhGalectin-8, recombinant mouse (rm) Galectin-1, rmGalectin-3, rmGalectin-4, and rmGalectin-7 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse Galectin‑9
Ala2-Thr353; aa 148-178 deletion
Accession # O08573
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Galectin‑9
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Galectin-9 antibody in Mouse Thymocytes antibody by Flow Cytometry. View Larger

Detection of Galectin-9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Goat Anti-Mouse Galectin-9 Short Isoform Affinity-purified Polyclonal Antibody (Catalog # AF3535, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunocytochemistry Galectin-9 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Galectin‑9 in Mouse Splenocytes. Galectin-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3535) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-9

Galectin-9, also known as Ecalectin, belongs to the Galectin family (S-type lectins) of galactoside-binding proteins. It is a 36‑39 kDa protein that lacks a secretion signal sequence, but can be secreted by a non-classical pathway. Galectin-9 is a tandem-repeat Galectin with two Galectin domains joined by a linker region. Two alternative spliced variants, a short and a long isoform, exist. Galectin-9 short isoform is expressed in a variety of tissues and cells, including fibroblasts, hepatocytes, endothelial cells and astrocytes. The long isoform, however, is expressed primarily in the intestine. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 shares 69% and 85% amino acid sequence identity with human and rat Galectin-9, respectively.

Entrez Gene IDs
3965 (Human); 16859 (Mouse)
Alternate Names
Ecalectin; GAL9; gal-9; galectin 9; Galectin9; Galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein

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Citations for Mouse Galectin-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Gangliosides modulate insulin secretion by pancreatic beta cells under glucose stress
    Authors: Richard Jennemann, Sylvia Kaden, Martina Volz, Viola Nordström, Silke Herzer, Roger Sandhoff et al.
  2. N-Linked Glycosylation Regulates CD22 Organization and Function
    Authors: Laabiah Wasim, Fathima Hifza Mohamed Buhari, Myuran Yoganathan, Taylor Sicard, June Ereño-Orbea, Jean-Philippe Julien et al.
    Frontiers in Immunology
  3. Characterization of neutralizing antibodies reacting with the 213-224 amino-acid segment of human galectin-9
    Authors: C Lhuillier, C Barjon, V Baloche, T Niki, A Gelin, R Mustapha, L Claër, S Hoos, Y Chiba, M Ueno, M Hirashima, M Wei, O Morales, B Raynal, N Delhem, O Dellis, P Busson
    PLoS ONE, 2018-09-11;13(9):e0202512.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  4. Galectin-9 is Critical for Mucosal Adaptive Immunity through the TH17-IgA Axis
    Authors: CC Liang, CS Li, IC Weng, HY Chen, HH Lu, CC Huang, FT Liu
    Am. J. Pathol., 2018-02-17;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture
  5. Astrocyte galectin-9 potentiates microglial TNF secretion.
    Authors: Steelman, Andrew J, Li, Jianrong
    J Neuroinflammation, 2014-08-27;11(0):144.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.
    Authors: Steelman A, Smith R, Welsh C, Li J
    J Biol Chem, 2013-07-08;288(33):23776-87.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. TIM-3 does not act as a receptor for galectin-9.
    Authors: Leitner, Judith, Rieger, Armin, Pickl, Winfried, Zlabinger, Gerhard, Grabmeier-Pfistershammer, Katharin, Steinberger, Peter
    PLoS Pathog, 2013-03-21;9(3):e1003253.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect.
    Authors: Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D
    Am. J. Pathol., 2011-03-01;178(3):1201-9.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot


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Mouse Galectin-9 Antibody
By Anonymous on 03/08/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult brain Species: Mouse

Mouse Galectin-9 Antibody
By Shubhankar Ambike on 02/24/2020
Application: WB Sample Tested: Cell Lysates Species: Mouse

Mouse alveolar macrophage- MH-S cell line. Total proteins were extracted from the cells and analysed for gal-9 expression by western blot. A lot of bands were seen and it was very difficult to determine which bands are the specific ones. According to the manual, bands boxed in a red rectangle are supposed to be the specific bands. Overall, the experiment was not at all conclusive. No bands were seen in supernatent as well. Not recommended to use for native expression detection in mouse macrophage cells.