Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all GDF-6/BMP-13 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse GDF-6/BMP-13
Thr335-Arg454
Accession # P43028

Specificity

Detects mouse GDF-6/BMP-13 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse GDF‑6/BMP‑13 Antibody

GDF-6/BMP-13 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

GDF‑6/BMP‑13 in Mouse Embryo.

GDF-6/BMP-13 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Mouse GDF-6/ BMP-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF855) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to dorsal neural tube. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse GDF‑6/BMP‑13 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GDF-6/BMP-13

Growth Differentiation Factor 6 (GDF-6), also known as bone morphogenetic protein 13 (BMP-13) or cartilage-derived morphogenetic protein 2 (CDMP-2), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. The mature GDF-6 with 120 amino acids is a homodimeric protein containing the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-6 is highly conserved across species. At the amino acid sequence level, human and mouse GDF‑6 are 99% identical. It has been reported that GDF-6 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-6 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

References

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
  5. Inada, M. et al. (1996) BBRC, 222:317.
  6. Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.

Long Name

Growth Differentiation Factor 6

Alternate Names

BMP-13, GDF6

Entrez Gene IDs

392255 (Human); 242316 (Mouse)

Gene Symbol

GDF6

UniProt

P43028 (Mouse)

Additional GDF-6/BMP-13 Products

Product Documents for Mouse GDF‑6/BMP‑13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse GDF‑6/BMP‑13 Antibody

For research use only

Customer Reviews for Mouse GDF‑6/BMP‑13 Antibody

There are currently no reviews for this product. Be the first to review Mouse GDF‑6/BMP‑13 Antibody and earn rewards!

Have you used Mouse GDF‑6/BMP‑13 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies