Mouse Granzyme B Antibody

Catalog # Availability Size / Price Qty
AF1865
AF1865-SP
Best Seller
Granzyme B in Mouse Splenocytes.
2 Images
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)

Mouse Granzyme B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Granzyme B in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Granzyme B
Lys17-Ser247
Accession # P04187
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Granzyme B (Catalog # 1865-SE)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Granzyme B (Catalog # 1865-SE), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Mouse Granzyme B Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Granzyme B Biotinylated Antibody (Catalog # BAF1865)

Standard: Recombinant Mouse Granzyme B Protein, CF (Catalog # 1865-SE)

Neutralization
Measured by its ability to neutralize Recombinant Mouse Granzyme B (0.5 µg/mL, Catalog # 1865-SE) cleavage of the fluorogenic peptide substrate Boc-AAD-SBzl (100 µM). The Neutralization Dose (ND50) is typically 3.5 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Granzyme B antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Granzyme B in Mouse Splenocytes. Granzyme B was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Granzyme B antibody in Mouse Spleen Tissue by Immunohistochemistry (IHC-P). View Larger

Granzyme B in Mouse Spleen Tissue. Granzyme B was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 8 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

References
  1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
  4. Lobe, C.G. et al. (1986) Science 232:858.
  5. Brunet, J.F. et al. (1986) Nature 322:268.
Entrez Gene IDs
3002 (Human); 14939 (Mouse)
Alternate Names
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GranzymeB; GRB; GrzB; GZMB; HLP; SECT

Product Datasheets

You must select a language.

x

Citations for Mouse Granzyme B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Preclinical PET Imaging of Granzyme B Shows Promotion of Immunological Response Following Combination Paclitaxel and Immune Checkpoint Inhibition in Triple Negative Breast Cancer
    Authors: TS Napier, CL Hunter, PN Song, BM Larimer, AG Sorace
    Pharmaceutics, 2022-02-18;14(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. ST3GAL2 knock-down decreases tumoral character of colorectal cancer cells in vitro and in vivo
    Authors: M Deschuyter, DY Leger, A Verboom, A Chaunavel, A Maftah, JM Petit
    American journal of cancer research, 2022-01-15;12(1):280-302.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. CD8+ tissue-resident memory T cells promote liver fibrosis resolution by inducing apoptosis of hepatic stellate cells
    Authors: Y Koda, T Teratani, PS Chu, Y Hagihara, Y Mikami, Y Harada, H Tsujikawa, K Miyamoto, T Suzuki, N Taniki, T Sujino, M Sakamoto, T Kanai, N Nakamoto
    Nature Communications, 2021-07-22;12(1):4474.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. IL-15, gluten and HLA-DQ8 drive tissue destruction in coeliac disease
    Authors: V Abadie, SM Kim, T Lejeune, BA Palanski, JD Ernest, O Tastet, J Voisine, V Discepolo, EV Marietta, MBF Hawash, C Ciszewski, R Bouziat, K Panigrahi, I Horwath, MA Zurenski, I Lawrence, A Dumaine, V Yotova, JC Grenier, JA Murray, C Khosla, LB Barreiro, B Jabri
    Nature, 2020-02-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. IL-6/JAK1 pathway drives PD-L1 Y112 phosphorylation to promote cancer immune evasion
    Authors: LC Chan, CW Li, W Xia, JM Hsu, HH Lee, JH Cha, HL Wang, WH Yang, EY Yen, WC Chang, Z Zha, SO Lim, YJ Lai, C Liu, J Liu, Q Dong, Y Yang, L Sun, Y Wei, L Nie, JL Hsu, H Li, Q Ye, MM Hassan, HM Amin, AO Kaseb, X Lin, SC Wang, MC Hung
    J. Clin. Invest., 2019-07-15;130(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. PARP inhibitor efficacy depends on CD8+ T cell recruitment via intratumoral STING pathway activation in BRCA-deficient models of triple-negative breast cancer
    Authors: C Pantelidou, O Sonzogni, M de Oliveir, AK Mehta, A Kothari, D Wang, T Visal, MK Li, J Pinto, JA Castrillon, EM Cheney, P Bouwman, J Jonkers, S Rottenberg, JL Guerriero, GM Wulf, GI Shapiro
    Cancer Discov, 2019-04-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Novel Recombinant DNA Vaccine Candidates for Human Respiratory Syncytial Virus: Preclinical Evaluation of Immunogenicity and Protection Efficiency
    Authors: MA Farrag, HM Amer, P Öhlschläge, ME Hamad, FN Almajhdi
    Hum Vaccin Immunother, 2017-03-08;0(0):0.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
    Authors: Whitehead M, Ohlschlager P, Almajhdi F, Alloza L, Marzabal P, Meyers A, Hitzeroth I, Rybicki E
    BMC Cancer, 2014-05-24;14(0):367.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  9. Endogenous retinoids in the pathogenesis of alopecia areata.
    Authors: Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H
    J Invest Dermatol, 2012-09-27;133(2):334-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Serine protease inhibitor 6 protects cytotoxic T cells from self-inflicted injury by ensuring the integrity of cytotoxic granules.
    Authors: Zhang M, Park SM, Wang Y, Shah R, Liu N, Murmann AE, Wang CR, Peter ME, Ashton-Rickardt PG
    Immunity, 2006-04-01;24(4):451-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse Granzyme B Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse Granzyme B Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse Granzyme B Antibody
By Katie Myers on 11/14/2022
Application: ELISA Sample Tested: Serum Species: Mouse

This antibody worked well as both a capture and detection molecule in our mouse ELISA. it was sensitive and had little background or cross-reactivity.