Mouse Granzyme B Antibody

R&D Systems | Catalog # AF1865

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, ELISA Development (Capture), Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Granzyme B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Granzyme B
Lys17-Ser247
Accession # P04187

Specificity

Detects mouse Granzyme B in ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Granzyme B Antibody

Granzyme B antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

Granzyme B in Mouse Splenocytes.

Granzyme B was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Granzyme B antibody in Mouse Spleen Tissue by Immunohistochemistry (IHC-P).

Granzyme B in Mouse Spleen Tissue.

Granzyme B was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 8 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Mouse Granzyme B ELISA Standard Curve

Mouse Granzyme B ELISA Standard Curve

Recombinant Mouse Granzyme B (Catalog # 1865-SE) was serially diluted and captured by Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Mouse Granzyme B ELISA Standard Curve

Mouse Granzyme B ELISA Standard Curve

Recombinant Mouse Granzyme B (Catalog # 1865-SE) was serially diluted and captured by Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of Granzyme B by Immunocytochemistry/ Immunofluorescence

Detection of Granzyme B by Immunocytochemistry/ Immunofluorescence

Combined treatment of FGFR4 inhibitor BLU-554 and CXCR2 inhibitor SB265610 dramatically decreased HOXB5-driven HCC metastasis. (A) The diagram of in vivo treatment in C57/BL mice. One week after injection of PLC-PRF/5-HOXB5 cells, mice in four group were treated with vehicle, BLU-554 or SB265610 or combined treatment respectively. (B-E) In vivo assays showed that combined treatment of FGFR4 and CXCR2 inhibitors can almost block HCC metastasis totally. (B) Representative Bioluminescence images, growth rate and lung metastasis rate were shown in different groups. (C) Metastatic lung nodules were shown. (D) Survival curve was shown in different mice. (E) HE staining shown lung metastatic nodules in different mice groups. (F) Flow cytometry showed the percent of MDSCs and CD8+T cells. (G) IF showed the infiltration of MDSCs and CD8+T cell in different groups. (H) A schematic diagram illustrated the importance of FGF19-HOXB5 signaling in HCC metastasis. FGF19-FGFR4 signaling upregulated HOXB5 expression through PI3K/Akt/HIF-1 alpha pathway. HOXB5 promoted HCC metastasis through transactivating FGFR4 and CXCL1. Combined FGFR4 inhibitor BLU-554 and CXCR2 inhibitor SB265610 almost abolished HOXB5-induced HCC metastasis. * P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33897880), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Granzyme B Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Granzyme B (Catalog # 1865-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Granzyme B (Catalog # 1865-SE)

Neutralization

Measured by its ability to neutralize Recombinant Mouse Granzyme B (0.5 µg/mL, Catalog # 1865-SE) cleavage of the fluorogenic peptide substrate Boc-AAD-SBzl (100 µM). The Neutralization Dose (ND50) is typically 3.5 µg/mL.

Mouse Granzyme B Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Granzyme B Biotinylated Antibody (Catalog # BAF1865)
  • Standard: Recombinant Mouse Granzyme B Protein, CF (Catalog # 1865-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF1865 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

References

  1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
  4. Lobe, C.G. et al. (1986) Science 232:858.
  5. Brunet, J.F. et al. (1986) Nature 322:268.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Entrez Gene IDs

3002 (Human); 14939 (Mouse)

Gene Symbol

GZMB

UniProt

P04187

Additional Granzyme B Products

Product Documents for Mouse Granzyme B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Granzyme B Antibody

For research use only

Citations for Mouse Granzyme B Antibody

Customer Reviews for Mouse Granzyme B Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse Granzyme B Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Mouse Granzyme B Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 11/14/2022
    This antibody worked well as both a capture and detection molecule in our mouse ELISA. it was sensitive and had little background or cross-reactivity.
    Mouse Granzyme B Antibody AF1865

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies