Mouse Granzyme B Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Granzyme B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) rhGranzyme A, rhGranzyme B, recombinant mouse (rm) Granzyme D, rmGranzyme G, and rhGranzyme H is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Granzyme B
    Lys17-Ser247
    Accession # P04187
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Granzyme B (Catalog # 1865-SE)
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Mouse Granzyme B (Catalog # 1865-SE), see our available Western blot detection antibodies
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Mouse Granzyme B Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse Granzyme B Antibody (Catalog # AF1865)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Granzyme B Biotinylated Antibody (Catalog # BAF1865)
  • ELISA Standard
     
    Recombinant Mouse Granzyme B Protein, CF (Catalog # 1865-SE)
  • Neutralization
    Measured by its ability to neutralize Recombinant Mouse Granzyme B (0.5 µg/mL, Catalog # 1865-SE) cleavage of the fluorogenic peptide substrate Boc-AAD-SBzl (100 µM). The Neutralization Dose (ND50) is typically 3.5 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Granzyme B in Mouse Splenocytes. Granzyme B was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

  • References:
    1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
    2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
    3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
    4. Lobe, C.G. et al. (1986) Science 232:858.
    5. Brunet, J.F. et al. (1986) Nature 322:268.
  • Entrez Gene IDs:
    3002 (Human); 14939 (Mouse)
  • Alternate Names:
    CSPB; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
    Authors: Whitehead M, Ohlschlager P, Almajhdi F, Alloza L, Marzabal P, Meyers A, Hitzeroth I, Rybicki E
    BMC Cancer, 2014;14(0):367.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ELISA Capture
  2. Endogenous retinoids in the pathogenesis of alopecia areata.
    Authors: Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H
    J Invest Dermatol, 2013;133(2):334-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  3. Serine protease inhibitor 6 protects cytotoxic T cells from self-inflicted injury by ensuring the integrity of cytotoxic granules.
    Authors: Zhang M, Park SM, Wang Y, Shah R, Liu N, Murmann AE, Wang CR, Peter ME, Ashton-Rickardt PG
    Immunity, 2006;24(4):451-61.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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