Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, ELISA Development (Capture), Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Granzyme B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Granzyme B
Lys17-Ser247
Accession # P04187

Specificity

Detects mouse Granzyme B in ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Granzyme B Antibody

Granzyme B antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

Granzyme B in Mouse Splenocytes.

Granzyme B was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Granzyme B antibody in Mouse Spleen Tissue by Immunohistochemistry (IHC-P).

Granzyme B in Mouse Spleen Tissue.

Granzyme B was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 8 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse Granzyme B Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Granzyme B (Catalog # 1865-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Granzyme B (Catalog # 1865-SE)

Neutralization

Measured by its ability to neutralize Recombinant Mouse Granzyme B (0.5 µg/mL, Catalog # 1865-SE) cleavage of the fluorogenic peptide substrate Boc-AAD-SBzl (100 µM). The Neutralization Dose (ND50) is typically 3.5 µg/mL.

Mouse Granzyme B Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Granzyme B Biotinylated Antibody (Catalog # BAF1865)
  • Standard: Recombinant Mouse Granzyme B Protein, CF (Catalog # 1865-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF1865 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

References

  1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
  4. Lobe, C.G. et al. (1986) Science 232:858.
  5. Brunet, J.F. et al. (1986) Nature 322:268.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Entrez Gene IDs

3002 (Human); 14939 (Mouse)

Gene Symbol

GZMB

UniProt

P04187

Additional Granzyme B Products

Product Documents for Mouse Granzyme B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Granzyme B Antibody

For research use only

Citations for Mouse Granzyme B Antibody

Customer Reviews for Mouse Granzyme B Antibody (1)

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  • Mouse Granzyme B Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 11/14/2022
    This antibody worked well as both a capture and detection molecule in our mouse ELISA. it was sensitive and had little background or cross-reactivity.
    Mouse Granzyme B Antibody AF1865

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Protocols

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