|Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 in Mouse Brain. Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5406) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.|
HS6ST3 (Heparin sulfate 6-O-sulfotransferase 3) is a 60-62 kDa member of the sulfotransferase 6 family of enzymes. It is one of three 6-O sulfotransferase isoforms, all of which essentially perform the same function, but all of which are the products of distinct genes. HS6ST3 is known to sulfate the #6 position of glucosamine sulfate that is linked to a 2-O-sulfated iduronic acid in heparin sulfate. The molecule shows a temporal expression pattern, being found in postnatal cerebellar Purkinje cells and cerebral cortical pyramidal cells in layers II/III and V. Mouse HS6ST3 is a 470 amino acid (aa) type II transmembrane glycoprotein. It contains a short four aa cytoplasmic N-terminus coupled to an extended 443 aa lumenal domain (aa 28-470). The lumenal domain contains a stem region (aa 28-83) that mediates oligomerization and Golgi localization, plus a sulfotransferase enzyme domain (aa 142-409). Cleavage within the stem region by beta -secretase generates a soluble form of HS6ST3. Over aa 28-470, mouse HS6ST3 shares 99% and 95% aa identity with rat and human HS6ST3, respectively.