Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3 in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3
    Pro28-Trp470
    Accession # NP_056635
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunohistochemistry
Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 in Mouse Brain. Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5406) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3

HS6ST3 (Heparin sulfate 6-O-sulfotransferase 3) is a 60-62 kDa member of the sulfotransferase 6 family of enzymes. It is one of three 6-O sulfotransferase isoforms, all of which essentially perform the same function, but all of which are the products of distinct genes. HS6ST3 is known to sulfate the #6 position of glucosamine sulfate that is linked to a 2-O-sulfated iduronic acid in heparin sulfate. The molecule shows a temporal expression pattern, being found in postnatal cerebellar Purkinje cells and cerebral cortical pyramidal cells in layers II/III and V. Mouse HS6ST3 is a 470 amino acid (aa) type II transmembrane glycoprotein. It contains a short four aa cytoplasmic N-terminus coupled to an extended 443 aa lumenal domain (aa 28-470). The lumenal domain contains a stem region (aa 28-83) that mediates oligomerization and Golgi localization, plus a sulfotransferase enzyme domain (aa 142-409). Cleavage within the stem region by beta -secretase generates a soluble form of HS6ST3. Over aa 28-470, mouse HS6ST3 shares 99% and 95% aa identity with rat and human HS6ST3, respectively.

  • Entrez Gene IDs:
    266722 (Human); 50787 (Mouse)
  • Alternate Names:
    DKFZp761K2315; heparan sulfate 6-O-sulfotransferase 3; HS6ST3
Related Research Areas

FAQs

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Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 2
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 5
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016 1
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Antigen Retrieval Reagent-Acidic

CTS014
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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NorthernLights Guard Mounting Media

NL996
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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 11
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB , Simple Western HAF016 17  
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL012 3
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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