Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody

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Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 in Mouse Brain.
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Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody Summary

Species Reactivity
Detects mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3 in direct ELISAs.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3
Accession # NP_056635
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 antibody in Mouse Brain  by Immunohistochemistry (IHC-Fr). View Larger

Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 in Mouse Brain. Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5406) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3

HS6ST3 (Heparin sulfate 6-O-sulfotransferase 3) is a 60-62 kDa member of the sulfotransferase 6 family of enzymes. It is one of three 6-O sulfotransferase isoforms, all of which essentially perform the same function, but all of which are the products of distinct genes. HS6ST3 is known to sulfate the #6 position of glucosamine sulfate that is linked to a 2-O-sulfated iduronic acid in heparin sulfate. The molecule shows a temporal expression pattern, being found in postnatal cerebellar Purkinje cells and cerebral cortical pyramidal cells in layers II/III and V. Mouse HS6ST3 is a 470 amino acid (aa) type II transmembrane glycoprotein. It contains a short four aa cytoplasmic N-terminus coupled to an extended 443 aa lumenal domain (aa 28-470). The lumenal domain contains a stem region (aa 28-83) that mediates oligomerization and Golgi localization, plus a sulfotransferase enzyme domain (aa 142-409). Cleavage within the stem region by beta -secretase generates a soluble form of HS6ST3. Over aa 28-470, mouse HS6ST3 shares 99% and 95% aa identity with rat and human HS6ST3, respectively.

Entrez Gene IDs
266722 (Human); 50787 (Mouse)
Alternate Names
DKFZp761K2315; heparan sulfate 6-O-sulfotransferase 3; HS6ST3

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