Mouse ICAM-5 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1173

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Mouse ICAM-5 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ICAM-5 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse (rm) ICAM-2, recombinant rat ICAM-1, and recombinant human (rh) ICAM-1 is observed and less than 2% cross‑reactivity with rhICAM-3, rmICAM-1, and rmVCAM-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑5
Leu31-Arg828 (Pro47Arg)
Accession # Q60625
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse ICAM‑5 Fc Chimera (Catalog # 1173-M5)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (hippocampus and cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM‑5), also known as telencephalin, is an integral membrane glycoprotein expressed in neurons of mammalian telencephalons. ICAM‑5 is a member of the immunoglobulin superfamily and shares 38‑55% amino acid homology with other ICAMs. Structurally, ICAM‑5 contains nine Ig domains that included 15 N-glycosylation sites, a single transmembrane region, and C‑terminal cytoplasmic tail (1). As with other members of the ICAM family, ICAM‑5 has been shown to be involved in cellular adhesion. ICAM‑5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) via its first NH2-terminal Ig domain. The ability of ICAM‑5 to bind LFA-1 suggests that ICAM‑5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM‑5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4‑5. Homophilic adhesion activity of ICAM‑5 is regulated by a monomer/tetramer transition. ICAM‑5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM‑5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).

Long Name
Intercellular Adhesion Molecule 5
Entrez Gene IDs
7087 (Human); 15898 (Mouse)
Alternate Names
ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin

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