Mouse/Rat ICAM-5 Antibody

Catalog # Availability Size / Price Qty
AF1173
AF1173-SP
Detection of Mouse and Rat ICAM‑5 by Western Blot.
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Product Details
Citations (3)
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Mouse/Rat ICAM-5 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse ICAM-5 in direct ELISAs. Detects mouse and rat ICAM-5 in  Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) ICAM-5 and recombinant mouse (rm) ICAM-2 is observed, 5% cross-reactivity with recombinant rat ICAM-1, rhICAM-1, and rhICAM-3 is observed and less than 1% cross-reactivity with rmICAM-1 and rmVCAM-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑5
Leu31-Arg828 (Pro47Arg)
Accession # Q60625
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (hippocampus and cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat ICAM-5 antibody by Western Blot. View Larger

Detection of Mouse and Rat ICAM‑5 by Western Blot. Western blot shows lysates of rat olfactory bulb tissue, mouse olfactory bulb tissue, mouse brain tissue, and human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse ICAM-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1173) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ICAM-5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is an integral membrane glycoprotein expressed in neurons of mammalian telencephalons. ICAM-5 is a member of the immunoglobulin superfamily and shares 38-55% amino acid homology with other ICAMs. Structurally, ICAM-5 contains nine Ig domains that included 15 N-glycosylation sites, a single transmembrane region, and C-terminal cytoplasmic tail (1). As with other members of the ICAM family, ICAM-5 has been shown to be involved in cellular adhesion. ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) via its first NH2-terminal Ig domain. The ability of
ICAM‑5 to bind LFA-1 suggests that ICAM-5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM-5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4-5. Homophilic adhesion activity of ICAM-5 is regulated by a monomer/tetramer transition. ICAM-5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM-5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).

References
  1. Tian, L. et al. (2000) Eur. J. Immunol. 30:810.
  2. Mizuno, T. and K. Mori (1997) J. Biol. Chem. 272:1156.
  3. Tian, L. et al. (2000) J. Cell Biol. 150:243.
Long Name
Intercellular Adhesion Molecule 5
Entrez Gene IDs
7087 (Human); 15898 (Mouse)
Alternate Names
ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin

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Citations for Mouse/Rat ICAM-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. MMPs and soluble ICAM-5 increase neuronal excitability within in vitro networks of hippocampal neurons.
    Authors: Niedringhaus M, Chen X, Dzakpasu R, Conant K
    PLoS ONE, 2012-08-13;7(8):e42631.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
    Authors: Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
    J. Neurochem., 2011-01-19;118(4):521-32.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  3. Matrix metalloproteinase-dependent shedding of intercellular adhesion molecule-5 occurs with long-term potentiation.
    Authors: Conant K, Wang Y, Szklarczyk A, Dudak A, Mattson MP, Lim ST
    Neuroscience, 2010-01-04;166(2):508-21.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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