Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat, Drosophila

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ICAM-5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑5
Leu31-Arg828 (Pro47Arg)
Accession # Q60625

Specificity

Detects mouse ICAM-5 in direct ELISAs. Detects mouse and rat ICAM-5 in  Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) ICAM-5 and recombinant mouse (rm) ICAM-2 is observed, 5% cross-reactivity with recombinant rat ICAM-1, rhICAM-1, and rhICAM-3 is observed and less than 1% cross-reactivity with rmICAM-1 and rmVCAM-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat ICAM‑5 Antibody

Detection of Mouse and Rat ICAM-5 antibody by Western Blot.

Detection of Mouse and Rat ICAM‑5 by Western Blot.

Western blot shows lysates of rat olfactory bulb tissue, mouse olfactory bulb tissue, mouse brain tissue, and human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse ICAM-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1173) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ICAM-5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse/Rat ICAM‑5 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus and cortex)

Western Blot

0.2 µg/mL
Sample: Rat olfactory bulb tissue, mouse olfactory bulb tissue, and mouse brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is an integral membrane glycoprotein expressed in neurons of mammalian telencephalons. ICAM-5 is a member of the immunoglobulin superfamily and shares 38-55% amino acid homology with other ICAMs. Structurally, ICAM-5 contains nine Ig domains that included 15 N-glycosylation sites, a single transmembrane region, and C-terminal cytoplasmic tail (1). As with other members of the ICAM family, ICAM-5 has been shown to be involved in cellular adhesion. ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) via its first NH2-terminal Ig domain. The ability of
ICAM‑5 to bind LFA-1 suggests that ICAM-5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM-5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4-5. Homophilic adhesion activity of ICAM-5 is regulated by a monomer/tetramer transition. ICAM-5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM-5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).

References

  1. Tian, L. et al. (2000) Eur. J. Immunol. 30:810.
  2. Mizuno, T. and K. Mori (1997) J. Biol. Chem. 272:1156.
  3. Tian, L. et al. (2000) J. Cell Biol. 150:243.

Long Name

Intercellular Adhesion Molecule 5

Alternate Names

ICAM5, Telencephalin

Entrez Gene IDs

7087 (Human); 15898 (Mouse)

Gene Symbol

ICAM5

UniProt

Q60625

Additional ICAM-5 Products

Product Documents for Mouse/Rat ICAM‑5 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse/Rat ICAM‑5 Antibody

For research use only

Citations for Mouse/Rat ICAM‑5 Antibody

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Protocols

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