Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

ELISA, Neutralization

Cited:

Immunohistochemistry, Neutralization, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # RMMA-1
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Product Specifications

Immunogen

Mouse Interferon alpha

Specificity

Neutralizes mouse interferon alpha; binds to mouse interferon alpha

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Endotoxin Level

<1.0 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse IFN‑ alpha Antibody

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Bicarbonate; 0.2M Sodium Chloride, pH 8.0

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Re-freezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

References

  1. Asselin-Paturel, C. et al. 2005, J. Exp. Med. 201:1157.
  2. Mellor, A.L. et al. 2005, J. Immunol. 175:5601.

Long Name

Interferon alpha

Alternate Names

IFNalpha, LeIF D

Entrez Gene IDs

3440 (Human)

Gene Symbol

IFNA2

Additional IFN-alpha Products

Product Documents for Mouse IFN‑ alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IFN‑ alpha Antibody

For research use only

Citations for Mouse IFN‑ alpha Antibody

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