Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Mouse IFN‑ alpha Antibody
R&D Systems | Catalog # 22100-1
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
ELISA, Neutralization
Cited:
Immunohistochemistry, Neutralization, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # RMMA-1
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Product Specifications
Immunogen
Mouse Interferon alpha
Specificity
Neutralizes mouse interferon alpha; binds to mouse interferon alpha
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Applications for Mouse IFN‑ alpha Antibody
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Bicarbonate; 0.2M Sodium Chloride, pH 8.0
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Re-freezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-alpha
References
- Asselin-Paturel, C. et al. 2005, J. Exp. Med. 201:1157.
- Mellor, A.L. et al. 2005, J. Immunol. 175:5601.
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Mouse IFN‑ alpha Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IFN‑ alpha Antibody
For research use only
Citations for Mouse IFN‑ alpha Antibody
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