Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

ELISA, Neutralization

Cited:

Immunohistochemistry, Neutralization, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # RMMA-1
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Product Specifications

Immunogen

Mouse Interferon alpha

Specificity

Neutralizes mouse interferon alpha; binds to mouse interferon alpha

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Endotoxin Level

<1.0 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse IFN-alpha Antibody (RMMA-1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Bicarbonate; 0.2M Sodium Chloride, pH 8.0

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Re-freezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

References

  1. Asselin-Paturel, C. et al. 2005, J. Exp. Med. 201:1157.
  2. Mellor, A.L. et al. 2005, J. Immunol. 175:5601.

Long Name

Interferon alpha

Alternate Names

IFNalpha, LeIF D

Entrez Gene IDs

3440 (Human)

Gene Symbol

IFNA2

Additional IFN-alpha Products

Product Documents for Mouse IFN-alpha Antibody (RMMA-1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IFN-alpha Antibody (RMMA-1)

For research use only

Citations for Mouse IFN-alpha Antibody (RMMA-1)

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