Mouse IFN-alpha / beta  R1 Biotinylated Antibody

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Product Details
Supplemental Products

Mouse IFN-alpha / beta  R1 Biotinylated Antibody Summary

Species Reactivity
Detects mouse IFN‑ alpha / beta R1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) IFN-alpha / beta R2, rmIFN-gamma R1 and rmIFN-gamma R2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R1
Accession # P33896
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse IFN-alpha / beta  R1 (Catalog # 3039-AB)
5-15 µg/mL
Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R1

IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1‑3). IFN-alpha / beta R1, in association with IFN-alpha / beta R2, is required for propagating antiviral signal transduction triggered by IFN-alpha and IFN-beta (4, 5). The mouse IFN-alpha / beta R1 cDNA encodes a 590 amino acid (aa) precursor including a 26 aa signal sequence, a 403 aa extracellular domain (ECD), a 20 aa transmembrane segment, and a 141 aa cytoplasmic domain (6). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. The ECD of mouse IFN-alpha / beta R1 shares 47‑48% aa identity with that of human, bovine, porcine, and ovine IFN-alpha / beta R1. IFN-alpha / beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN-alpha / beta R2. Ligands associate with IFN-alpha / beta R2, and this complex subsequently forms a stable ternary assembly with IFN-alpha / beta R1 (7, 8). IFN-alpha / beta R1 also associates with IFN-gamma R2 even in the absence of IFN-gamma stimulation (5). Tyrosine phosphorylation within the juxtamembrane cytoplasmic domain of IFN-alpha / beta R1 provides a docking site for the SH2 domains of Tyk2 and STAT2 (9‑11). Tyk2 can directly phosphorylate IFN-alpha / beta R1 (10). Tyk2 also increases the level of cell surface expression of IFN-alpha / beta R1 by preventing constitutive internalization (12). Human IFN-alpha / beta R1 contains a nuclear localization signal in its ECD which is required for receptor translocation to the nucleus following interaction with ligand (13).

  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Kotenko, S.V. and J.A. Langer (2004) Int. Immunopharmacol. 4:593.
  3. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  4. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284.
  5. Takaoka, A. et al. (2000) Science 288:2357.
  6. Uze, G. et al. (1992) Proc. Natl. Acad. Sci. USA 89:4774.
  7. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
  8. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
  9. Krishnan, K. et al. (1998) J. Biol. Chem. 273:19495.
  10. Yan, H. et al. (1996) Mol. Cell. Biol. 16:2074.
  11. Yan, H. et al. (1996) EMBO J. 15:1064.
  12. Ragimbeau, J. et al. (2003) EMBO J. 22:537.
  13. Subramaniam, P.S. and H.M. Johnson (2004) FEBS Lett. 578:207.
Long Name
Interferon alpha/beta Receptor 1
Entrez Gene IDs
3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)
Alternate Names
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1

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