Mouse IFN-gamma Quantikine ELISA Kit MIF00: R&D Systems
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL)
  • Sensitivity
    2 pg/mL
  • Assay Range
    9.4 - 600 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant mouse IFN-gamma
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-gamma in cell culture supernates and mouse serum. It contains E. coli-expressed mouse IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IFN-gamma accurately. Results obtained using natural mouse IFN-gamma showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-gamma.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean45.114352842.8139518
Standard Deviation2.14.411.83.613.646.5
CV%4.73.12.28.49.89

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean44.815651843.3144509
Standard Deviation2.2514.23.614.748.6
CV%4.93.22.78.310.29.5

Recovery

The recovery of mouse IFN-gamma spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 105 98-115
Serum (n=5) 97 91-106
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IFN-gamma in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
Mouse IFN-gamma Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-gamma
IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.
    • Long Name
      Interferon gamma
    • Entrez Gene IDs
      3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline);
    • Alternate Names
      IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    177 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Host resistance to Plasmodium-induced acute immune pathology is regulated by IL-10 receptor signalling
      Authors: C Claser, JB De Souza, SG Thorburn, GE Grau, EM Riley, L Rénia, JC Hafalla
      Infect. Immun., 2017;0(0):.
      Species: Mouse
      Sample Type: Serum
    2. Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model
      Authors: S Ishikawa, T Ishikawa, C Tezuka, K Asano, M Sunagawa, T Hisamitsu
      Evid Based Complement Alternat Med, 2017;2017(0):6054706.
      Species: Mouse
      Sample Type: Serum
    3. Cutting Edge: Innate Immune Augmenting Vesicular Stomatitis Virus Expressing Zika Virus Proteins Confers Protective Immunity
      Authors: D Betancourt, NM de Queiroz, T Xia, J Ahn, GN Barber
      J. Immunol, 2017;0(0):.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    4. Immune Regulation of Intrahepatic Regulatory T Cells in Fibrotic Livers of Mice
      Authors: X Zhang, J Lou, L Bai, Y Chen, S Zheng, Z Duan
      Med. Sci. Monit, 2017;23(0):1009-1016.
      Species: Mouse
      Sample Type: Serum
    5. Induction and characterization of anti-tumor endothelium immunity elicited by ValloVax therapeutic cancer vaccine
      Authors: SC Wagner, TE Ichim, V Bogin, WP Min, F Silva, AN Patel, S Kesari
      Oncotarget, 2017;8(17):28595-28613.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T�cells and T-bet/GATA-3 expression in NOD mice
      Authors: A Tang, C Li, Z Chen, T Li
      Mol Med Rep, 2017;0(0):.
      Species: Mouse
      Sample Type: Serum
    7. Effects of Gintonin-Enriched Fraction in an Atopic Dermatitis Animal Model: Involvement of Autotaxin Regulation
      Authors: BH Lee, HK Kim, M Jang, HJ Kim, SH Choi, SH Hwang, HC Kim, H Rhim, IH Cho, SY Nah
      Biol. Pharm. Bull., 2017;40(7):1063-1070.
      Species: Mouse
      Sample Type: Serum
    8. Protective Efficacy of the Trivalent Pseudomonas aeruginosa Vaccine Candidate PcrV-OprI-Hcp1 in Murine Pneumonia and Burn Models
      Authors: F Yang, J Gu, L Yang, C Gao, H Jing, Y Wang, H Zeng, Q Zou, F Lv, J Zhang
      Sci Rep, 2017;7(1):3957.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
      Authors: U Lampreht T, L Loiacono, M Cemazar, U Kamensek, VM Fazio, G Sersa, E Signori
      Mediators Inflamm., 2017;2017(0):5285890.
      Species: Mouse
      Sample Type: Serum
    10. Lamtor1 Is Critically Required for CD4(+) T Cell Proliferation and Regulatory T Cell Suppressive Function
      Authors: T Hosokawa, T Kimura, S Nada, T Okuno, D Ito, S Kang, S Nojima, K Yamashita, T Nakatani, Y Hayama, Y Kato, Y Kinehara, M Nishide, N Mikami, S Koyama, H Takamatsu, D Okuzaki, N Ohkura, S Sakaguchi, M Okada, A Kumanogoh
      J. Immunol., 2017;0(0):.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    11. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
      Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
      J Microbiol Immunol Infect, 2017;0(0):.
      Species: Mouse
      Sample Type: BALF
    12. Vaccination with the Secreted Glycoprotein G of Herpes Simplex Virus 2 Induces Protective Immunity after Genital Infection
      Authors: K Önnheim, M Ekblad, S Görander, T Bergström, JÅ Liljeqvist
      Viruses, 2016;8(4):.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    13. In Vivo Expansion of Activated Foxp3+ Regulatory T Cells and Establishment of a Type 2 Immune Response upon IL-33 Treatment Protect against Experimental Arthritis
      J Immunol, 2016;0(0):.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    14. Distinct Roles of Type I and Type III Interferons in Intestinal Immunity to Homologous and Heterologous Rotavirus Infections
      PLoS Pathog, 2016;12(4):e1005600.
      Species: Mouse
      Sample Type: Tissue Homogenates
    15. Intravenous hMSCs Ameliorate Acute Pancreatitis in Mice via Secretion of Tumor Necrosis Factor-? Stimulated Gene/Protein 6
      Sci Rep, 2016;6(0):38438.
      Species: Mouse
      Sample Type: Serum
    16. Th1 cells downregulate connexin 43 gap junctions in astrocytes via microglial activation
      Sci Rep, 2016;6(0):38387.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    17. Propionibacterium acnes Augments Antitumor, Anti-Angiogenesis and Immunomodulatory Effects of Melatonin on Breast Cancer Implanted in Mice.
      Authors: Talib W, Saleh S
      PLoS ONE, 2015;10(4):e0124384.
      Species: Mouse
      Sample Type: Serum
    18. Use of Recombinant Virus Replicon Particles for Vaccination against Mycobacterium ulcerans Disease.
      Authors: Bolz M, Kerber S, Zimmer G, Pluschke G
      PLoS Negl Trop Dis, 2015;9(8):e0004011.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    19. HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity.
      Authors: Wang H, Sun R, Li Y, Yang Y, Xiao F, Zhang Y, Wang S, Sun H, Zhang Q, Wu C, Wang L
      PLoS ONE, 2015;10(5):e0124420.
      Species: Mouse
      Sample Type: Serum
    20. IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance.
      Authors: Basu R, Whitley S, Bhaumik S, Zindl C, Schoeb T, Benveniste E, Pear W, Hatton R, Weaver C
      Nat Immunol, 2015;16(3):286-95.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    21. Modulation of recombinant antigenic constructs containing multi-epitopes towards effective reduction of atherosclerotic lesion in B6;129S-Ldlr(tm1Her)Apob(tm2Sgy)/J mice.
      Authors: Xia M, Chen D, Endresz V, Lantos I, Szabo A, Kakkar V, Lu X
      PLoS ONE, 2015;10(4):e0123393.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    22. Absence of the Adaptor Protein PEA-15 Is Associated with Altered Pattern of Th Cytokines Production by Activated CD4+ T Lymphocytes In Vitro, and Defective Red Blood Cell Alloimmune Response In Vivo.
      Authors: Kerbrat S, Vingert B, Junier M, Castellano F, Renault-Mihara F, Dos Reis Tavares S, Surenaud M, Noizat-Pirenne F, Boczkowski J, Guellaen G, Chneiweiss H, Le Gouvello S
      PLoS ONE, 2015;10(8):e0136885.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    23. WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features.
      Authors: Zhao D, Xu A, Dai Z, Peng J, Zhu M, Shen J, Zheng Q, Ran Z
      BMC Gastroenterol, 2015;15(0):173.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    24. Immunomodulator-based enhancement of anti smallpox immune responses.
      Authors: Martinez O, Miranda E, Ramirez M, Santos S, Rivera C, Vazquez L, Sanchez T, Tremblay R, Rios-Olivares E, Otero M
      PLoS ONE, 2015;10(4):e0123113.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    25. Efficient expression of bioactive murine IL12 as a self-processing P2A polypeptide driven by inflammation-regulated promoters in tumor cell lines.
      Authors: Lorenzo C, Perez-Chacon G, Garaulet G, Mallorquin Z, Zapata J, Rodriguez A
      Cancer Gene Ther, 2015;22(11):542-51.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    26. G-protein-coupled estrogen receptor agonist suppresses airway inflammation in a mouse model of asthma through IL-10.
      Authors: Itoga M, Konno Y, Moritoki Y, Saito Y, Ito W, Tamaki M, Kobayashi Y, Kayaba H, Kikuchi Y, Chihara J, Takeda M, Ueki S, Hirokawa M
      PLoS ONE, 2015;10(3):e0123210.
      Species: Mouse
      Sample Type: BALF
    27. Interferon Gamma, but not Calcitriol Improves the Osteopetrotic Phenotypes in ADO2 Mice.
      Authors: Alam I, Gray A, Acton D, Gerard-O'Riley R, Reilly A, Econs M
      J Bone Miner Res, 2015;30(11):2005-13.
      Species: Mouse
      Sample Type: Serum
    28. Thrombomodulin alleviates murine GVHD in association with an increase in the proportion of regulatory T cells in the spleen.
      Authors: Ikezoe T, Yang J, Nishioka C, Yokoyama A
      Bone Marrow Transplant, 2015;50(1):113-20.
      Species: Mouse
      Sample Type: Plasma
    29. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
      Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
      Nat Commun, 2015;6(0):6224.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    30. ASK1 promotes the contact hypersensitivity response through IL-17 production.
      Authors: Mizukami J, Sato T, Camps M, Ji H, Rueckle T, Swinnen D, Tsuboi R, Takeda K, Ichijo H
      Sci Rep, 2014;4(0):4714.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    31. Axl receptor blockade ameliorates pulmonary pathology resulting from primary viral infection and viral exacerbation of asthma.
      Authors: Shibata T, Habiel D, Coelho A, Kunkel S, Lukacs N, Hogaboam C
      J Immunol, 2014;192(8):3569-81.
      Species: Mouse
      Sample Type: Whole Tissue
    32. PD-1 blockade and OX40 triggering synergistically protects against tumor growth in a murine model of ovarian cancer.
      Authors: Guo Z, Wang X, Cheng D, Xia Z, Luan M, Zhang S
      PLoS ONE, 2014;9(2):e89350.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    33. Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.
      Authors: Hu S, Rotschafer J, Lokensgard J, Cheeran M
      PLoS ONE, 2014;9(8):e105219.
      Species: Mouse
      Sample Type: Whole Cells
    34. Diisopropylamine dichloroacetate, a novel pyruvate dehydrogenase kinase 4 inhibitor, as a potential therapeutic agent for metabolic disorders and multiorgan failure in severe influenza.
      Authors: Yamane, Kazuhiko, Indalao, Irene L, Chida, Junji, Yamamoto, Yoshikaz, Hanawa, Masaaki, Kido, Hiroshi
      PLoS ONE, 2014;9(5):e98032.
      Species: Mouse
      Sample Type: Tissue Homogenates
    35. Combined PD-1 blockade and GITR triggering induce a potent antitumor immunity in murine cancer models and synergizes with chemotherapeutic drugs.
      Authors: Lu L, Xu X, Zhang B, Zhang R, Ji H, Wang X
      J Transl Med, 2014;12(0):36.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    36. Oncolytic viral therapy with a combination of HF10, a herpes simplex virus type 1 variant and granulocyte-macrophage colony-stimulating factor for murine ovarian cancer.
      Authors: Goshima F, Esaki S, Luo C, Kamakura M, Kimura H, Nishiyama Y
      Int J Cancer, 2014;134(12):2865-77.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    37. Leishmania donovani targets tumor necrosis factor receptor-associated factor (TRAF) 3 for impairing TLR4-mediated host response.
      Authors: Gupta P, Giri J, Srivastav S, Chande A, Mukhopadhyaya R, Das P, Ukil A
      FASEB J, 2014;28(4):1756-68.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    38. Induction of cell-mediated immune responses in mice by DNA vaccines that express hepatitis C virus NS3 mutants lacking serine protease and NTPase/RNA helicase activities.
      Authors: Ratnoglik S, Jiang D, Aoki C, Sudarmono P, Shoji I, Deng L, Hotta H
      PLoS ONE, 2014;9(6):e98877.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    39. Sindbis viral vectors transiently deliver tumor-associated antigens to lymph nodes and elicit diversified antitumor CD8+ T-cell immunity.
      Authors: Granot, Tomer, Yamanashi, Yoshihid, Meruelo, Daniel
      Mol Ther, 2014;22(1):112-22.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    40. IL-18 expression results in a recombinant vaccinia virus that is highly attenuated and immunogenic.
      Authors: Verardi P, Legrand F, Chan K, Peng Y, Jones L, Yilma T
      J Interferon Cytokine Res, 2014;34(3):169-78.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    41. Genetic vaccine for respiratory syncytial virus provides protection without disease potentiation.
      Authors: Johnson T, Rangel D, Graham B, Brough D, Gall J
      Mol Ther, 2014;22(1):196-205.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    42. Antibody to mCLCA3 suppresses symptoms in a mouse model of asthma.
      Authors: Song L, Liu D, Wu C, Wu S, Yang J, Ren F, Li Y
      PLoS ONE, 2013;8(12):e82367.
      Species: Mouse
      Sample Type: BALF
    43. Combinatorial PD-1 blockade and CD137 activation has therapeutic efficacy in murine cancer models and synergizes with cisplatin.
      Authors: Wei H, Zhao L, Li W, Fan K, Qian W, Hou S, Wang H, Dai M, Hellstrom I, Hellstrom K, Guo Y
      PLoS ONE, 2013;8(12):e84927.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    44. Decorin potentiates interferon-gamma activity in a model of allergic inflammation.
      Authors: Bocian C, Urbanowitz A, Owens R, Iozzo R, Gotte M, Seidler D
      J Biol Chem, 2013;288(18):12699-711.
      Species: Mouse
      Sample Type: Tissue Homogenates
    45. Targeted inhibition of serotonin type 7 (5-HT7) receptor function modulates immune responses and reduces the severity of intestinal inflammation.
      Authors: Kim, Janice J, Bridle, Byram W, Ghia, Jean-Eri, Wang, Huaqing, Syed, Shahzad, Manocha, Marcus M, Rengasamy, Palanive, Shajib, Mohammad, Wan, Yonghong, Hedlund, Peter B, Khan, Waliul I
      J Immunol, 2013;190(9):4795-804.
      Species: Mouse
      Sample Type: Tissue Homogenates
    46. TLR2 and Nod2 mediate resistance or susceptibility to fatal intracellular Ehrlichia infection in murine models of ehrlichiosis.
      Authors: Chattoraj P, Yang Q, Khandai A, Al-Hendy O, Ismail N
      PLoS ONE, 2013;8(3):e58514.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    47. The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
      Authors: Calderon J, Maganto-Garcia E, Punzon C, Carrion J, Terhorst C, Fresno M
      PLoS Pathog., 2012;8(7):e1002799.
      Species: Mouse
      Sample Type: Serum
    48. Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease.
      Authors: Gottfries J, Melgar S, Michaelsson E
      PLoS ONE, 2012;7(1):e30005.
      Species: Mouse
      Sample Type: Tissue Homogenates
    49. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
      Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
      Am. J. Pathol., 2012;179(1):302-14.
      Species: Mouse
      Sample Type: Tissue Homogenates
    50. Dendritic cell exosomes directly kill tumor cells and activate natural killer cells via TNF superfamily ligands.
      Authors: Munich S, Sobo-Vujanovic A, Buchser W, Beer-Stolz D, Vujanovic N
      Oncoimmunology, 2012;1(7):1074-1083.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    51. Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
      Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
      J. Biol. Chem., 2012;287(20):16356-64.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    52. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
      Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
      J. Immunol., 2011;188(3):1503-13.
      Species: Mouse
      Sample Type: BALF
    53. Over-expression of the LTC4 synthase gene in mice reproduces human aspirin-induced asthma.
      Authors: Hirata H, Arima M, Fukushima Y, Honda K, Sugiyama K, Tokuhisa T, Fukuda T
      Clin. Exp. Allergy, 2011;41(8):1133-42.
      Species: Mouse
      Sample Type: BALF
    54. Control of mouse graft-versus-host disease following allogeneic bone marrow transplantation by blocking the CD28/B7 signaling pathway with lentiviral vector-mediated RNA interference.
      Authors: Sang W, Zhou C, Cheng N, Li Z, Zeng L, Xu K
      Immunol. Lett., 2011;136(2):194-202.
      Species: Mouse
      Sample Type: Plasma
    55. hsBAFF regulates proliferation and response in cultured CD4(+) T lymphocytes by upregulation of intracellular free Ca(2+) homeostasis.
      Authors: Wen L, Chen SJ, Zhang W, Ma HW, Zhang SQ, Chen L
      Cytokine, 2011;53(2):215-22.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    56. IL-33-activated dendritic cells are critical for allergic airway inflammation.
      Authors: Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B
      Eur. J. Immunol., 2011;41(6):1675-86.
      Species: Mouse
      Sample Type: BALF
    57. Differential host determinants contribute to the pathogenesis of 2009 pandemic H1N1 and human H5N1 influenza A viruses in experimental mouse models.
      Authors: Otte A, Sauter M, Alleva L, Baumgarte S, Klingel K, Gabriel G
      Am. J. Pathol., 2011;179(1):230-9.
      Species: Mouse
      Sample Type: Serum
    58. The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines.
      Authors: Vultaggio A, Nencini F, Pratesi S, Maggi L, Guarna A, Annunziato F, Romagnani S, Parronchi P, Maggi E
      J. Immunol., 2011;186(8):4707-15.
      Species: Mouse
      Sample Type: Serum
    59. Crawling Phagocytes Recruited in the Brain Vasculature after Pertussis Toxin Exposure through IL6, ICAM1 and ITGalphaM.
      Authors: Richard JF, Roy M, Audoy-Remus J, Tremblay P, Vallieres L
      Brain Pathol., 2011;21(0):661.
      Species: Mouse
      Sample Type: Serum
    60. Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
      Authors: Sun J, Fu F, Gu W, Yan R, Zhang G, Shen Z, Zhou Y, Wang H, Shen B, Zhang X
      PLoS ONE, 2011;6(9):e24751.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    61. The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
      Authors: Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N
      J. Immunol., 2011;187(3):1333-46.
      Species: Mouse
      Sample Type: Serum
    62. Serotonin activates dendritic cell function in the context of gut inflammation.
      Authors: Li N, Ghia JE, Wang H, McClemens J, Cote F, Suehiro Y, Mallet J, Khan WI
      Am. J. Pathol., 2011;178(2):662-71.
    63. Inhibition of lymphocyte trafficking shields the brain against deleterious neuroinflammation after stroke.
      Authors: Liesz A, Zhou W, Mracsko É, Karcher S, Bauer H, Schwarting S, Sun L, Bruder D, Stegemann S, Cerwenka A, Sommer C, Dalpke AH, Veltkamp R
      Brain, 2011;134(0):704-20.
      Species: Mouse
      Sample Type: Serum
    64. Deletion of tristetraprolin caused spontaneous reactive granulopoiesis by a non-cell-autonomous mechanism without disturbing long-term hematopoietic stem cell quiescence.
      Authors: Kaplan IM, Morisot S, Heiser D, Cheng WC, Kim MJ, Civin CI
      J. Immunol., 2011;186(5):2826-34.
      Species: Mouse
      Sample Type: Plasma
    65. Microbial exposure early in life regulates airway inflammation in mice after infection with Streptococcus pneumoniae with enhancement of local resistance.
      Authors: Yasuda Y, Matsumura Y, Kasahara K, Ouji N, Sugiura S, Mikasa K, Kita E
      Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(1):L67-78.
      Species: Mouse
      Sample Type: BALF
    66. Enhanced production of IL-17A during zymosan-induced peritonitis in obese mice.
      Authors: Pini M, Fantuzzi G
      J. Leukoc. Biol., 2010;87(1):51-8.
      Species: Mouse
      Sample Type: Peritoneal Lavage
    67. Absence or inhibition of matrix metalloproteinase-8 decreases ventilator-induced lung injury.
      Authors: Albaiceta GM, Gutierrez-Fernandez A, Garcia-Prieto E, Puente XS, Parra D, Astudillo A, Campestre C, Cabrera S, Gonzalez-Lopez A, Fueyo A, Taboada F, Lopez-Otin C
      Am. J. Respir. Cell Mol. Biol., 2010;43(5):555-63.
      Species: Mouse
      Sample Type: Tissue Homogenates
    68. Epithelial reticulon 4B (Nogo-B) is an endogenous regulator of Th2-driven lung inflammation.
      Authors: Wright PL, Yu J, Di YP
      J. Exp. Med., 2010;207(12):2595-607.
      Species: Mouse
      Sample Type: BALF
    69. Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages.
      Authors: Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA
      J. Immunol., 2010;185(4):2044-50.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    70. Mucosal vaccination using claudin-4-targeting.
      Authors: Kakutani H, Kondoh M, Fukasaka M, Suzuki H, Hamakubo T, Yagi K
      Biomaterials, 2010;31(20):5463-71.
      Species: Mouse
      Sample Type: Serum
    71. IL-7 promotes T cell viability, trafficking, and functionality and improves survival in sepsis.
      Authors: Unsinger J, McGlynn M, Kasten KR, Hoekzema AS, Watanabe E, Muenzer JT, McDonough JS, Tschoep J, Ferguson TA, McDunn JE, Morre M, Hildeman DA, Caldwell CC, Hotchkiss RS
      J. Immunol., 2010;184(7):3768-79.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    72. Antigen-specific Th9 cells exhibit uniqueness in their kinetics of cytokine production and short retention at the inflammatory site.
      Authors: Tan C, Aziz MK, Lovaas JD, Vistica BP, Shi G, Wawrousek EF, Gery I
      J. Immunol., 2010;185(11):6795-801.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    73. Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model.
      Authors: Yoshizaki A, Yanaba K, Iwata Y
      J. Immunol., 2010;185(4):2502-15.
      Species: Mouse
      Sample Type: Serum
    74. Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production.
      Authors: Gueders MM, Paulissen G, Crahay C, Quesada-Calvo F, Hacha J, Van Hove C, Tournoy K, Louis R, Foidart JM, Noel A, Cataldo DD
      Inflamm. Res., 2009;58(12):845-54.
      Species: Mouse
      Sample Type: BALF
    75. A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
      Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
      J. Immunol., 2009;183(2):1291-300.
      Species: Mouse
      Sample Type: BALF
    76. Differential IL-23 requirement for IL-22 and IL-17A production during innate immunity against Salmonella enterica serovar Enteritidis.
      Authors: Siegemund S, Schutze N, Schulz S, Wolk K, Nasilowska K, Straubinger RK, Sabat R, Alber G
      Int. Immunol., 2009;21(5):555-65.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    77. CCL2/MCP-1 controls parasite burden, cell infiltration, and mononuclear activation during acute Trypanosoma cruzi infection.
      Authors: Paiva CN, Figueiredo RT, Kroll-Palhares K, Silva AA, Silverio JC, Gibaldi D, Pyrrho Ados S, Benjamim CF, Lannes-Vieira J, Bozza MT
      J. Leukoc. Biol., 2009;86(5):1239-46.
      Species: Mouse
      Sample Type: Plasma
    78. Macrophage migration inhibitory factor homologs of anisakis simplex suppress Th2 response in allergic airway inflammation model via CD4+CD25+Foxp3+ T cell recruitment.
      Authors: Park SK, Cho MK, Park HK, Lee KH, Lee SJ, Choi SH, Ock MS, Jeong HJ, Lee MH, Yu HS
      J. Immunol., 2009;182(11):6907-14.
      Species: Mouse
      Sample Type: BALF
    79. A small molecule CRTH2 antagonist inhibits FITC-induced allergic cutaneous inflammation.
      Authors: Boehme SA, Franz-Bacon K, Chen EP, Sasik R, Sprague LJ, Ly TW, Hardiman G, Bacon KB
      Int. Immunol., 2009;21(1):81-93.
      Species: Mouse
      Sample Type: Tissue Homogenates
    80. Modified adenine (9-benzyl-2-butoxy-8-hydroxyadenine) redirects Th2-mediated murine lung inflammation by triggering TLR7.
      Authors: Vultaggio A, Nencini F, Fitch PM, Fili L, Maggi L, Fanti P, deVries A, Beccastrini E, Palandri F, Manuelli C, Bani D, Giudizi MG, Guarna A, Annunziato F, Romagnani S, Maggi E, Howie SE, Parronchi P
      J. Immunol., 2009;182(2):880-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    81. TLR9 activation coupled to IL-10 deficiency induces adverse pregnancy outcomes.
      Authors: Thaxton JE, Romero R, Sharma S
      J. Immunol., 2009;183(2):1144-54.
      Species: Mouse
      Sample Type: Serum
    82. Selective triggering of apoptosis of concanavalin A-activated T cells by fraxinellone for the treatment of T-cell-dependent hepatitis in mice.
      Authors: Sun Y, Qin Y, Gong FY, Wu XF, Hua ZC, Chen T, Xu Q
      Biochem. Pharmacol., 2009;77(11):1717-24.
      Species: Mouse
      Sample Type: Serum
    83. Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors.
      Authors: Ghiringhelli F, Apetoh L, Tesniere A, Aymeric L, Ma Y, Ortiz C, Vermaelen K, Panaretakis T, Mignot G, Ullrich E, Perfettini JL, Schlemmer F, Tasdemir E, Uhl M, Genin P, Civas A, Ryffel B, Kanellopoulos J, Tschopp J, Andre F, Lidereau R, McLaughlin NM, Haynes NM, Smyth MJ, Kroemer G, Zitvogel L
      Nat. Med., 2009;15(10):1170-8.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    84. Celastrol suppresses allergen-induced airway inflammation in a mouse allergic asthma model.
      Authors: Kim DY, Park JW, Jeoung D, Ro JY
      Eur. J. Pharmacol., 2009;612(1):98-105.
      Species: Mouse
      Sample Type: BALF
    85. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
      Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
      Vaccine, 2009;27(23):3045-52.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    86. Neutralization of Interleukin-18 Ameliorates Ischemia/Reperfusion-induced Myocardial Injury.
      Authors: Venkatachalam K, Prabhu SD, Reddy VS, Boylston WH, Valente AJ, Chandrasekar B
      J. Biol. Chem., 2009;284(12):7853-65.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    87. Vasoactive intestinal peptide attenuates concanavalin A-mediated liver injury.
      Authors: Luo Q, Wang Y, Feng D, Xu Y, Xu L
      Eur. J. Pharmacol., 2009;607(1):226-33.
      Species: Mouse
      Sample Type: Plasma
    88. The adjuvancy of OX40 ligand (CD252) on an HIV-1 canarypox vaccine.
      Authors: Liu J, Ngai N, Stone GW, Yue FY, Ostrowski MA
      Vaccine, 2009;27(37):5077-84.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    89. Simultaneous absence of surfactant proteins A and D increases lung inflammation and injury after allogeneic HSCT in mice.
      Authors: Gram K, Yang S, Steiner M, Somani A, Hawgood S, Blazar BR, Panoskaltsis-Mortari A, Haddad IY
      Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(2):L167-75.
      Species: Mouse
      Sample Type: BALF
    90. Stearoyl-CoA desaturase 1 deficiency protects mice from immune-mediated liver injury.
      Authors: Feng D, Wang Y, Mei Y, Xu Y, Xu H, Lu Y, Luo Q, Zhou S, Kong X, Xu L
      Lab. Invest., 2009;89(2):222-30.
      Species: Mouse
      Sample Type: Plasma
    91. Acid sphingomyelinase is a key regulator of cytotoxic granule secretion by primary T lymphocytes.
      Authors: Herz J, Pardo J, Kashkar H, Schramm M, Kuzmenkina E, Bos E, Wiegmann K, Wallich R, Peters PJ, Herzig S, Schmelzer E, Kronke M, Simon MM, Utermohlen O
      Nat. Immunol., 2009;10(7):761-8.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    92. Effects of a leukotriene B4 receptor antagonist on bleomycin-induced pulmonary fibrosis.
      Authors: Izumo T, Kondo M, Nagai A
      Eur. Respir. J., 2009;34(6):1444-51.
      Species: Mouse
      Sample Type: BALF
    93. A severe deficiency of coagulation factor VIIa results in attenuation of the asthmatic response in mice.
      Authors: Shinagawa K, Ploplis VA, Castellino FJ
      Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L763-70.
      Species: Mouse
      Sample Type: BALF
    94. Staphylococcus aureus infection after splenectomy and splenic autotransplantation in BALB/c mice.
      Authors: Teixeira FM, Fernandes BF, Rezende AB, Machado RR, Alves CC, Perobelli SM, Nunes SI, Farias RE, Rodrigues MF, Ferreira AP, Oliveira SC, Teixeira HC
      Clin. Exp. Immunol., 2008;154(2):255-63.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    95. Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis.
      Authors: Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T
      J. Immunol., 2008;181(6):4208-18.
      Species: Mouse
      Sample Type: Peritoneal Lavage
    96. NOD2-deficient mice have impaired resistance to Mycobacterium tuberculosis infection through defective innate and adaptive immunity.
      Authors: Divangahi M, Mostowy S, Coulombe F, Kozak R, Guillot L, Veyrier F, Kobayashi KS, Flavell RA, Gros P, Behr MA
      J. Immunol., 2008;181(10):7157-65.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    97. Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein.
      Authors: Johnson TR, Rothenberg ME, Graham BS
      J. Leukoc. Biol., 2008;84(3):748-59.
      Species: Mouse
      Sample Type: Tissue Homogenates
    98. Cigarette smoke selectively enhances viral PAMP- and virus-induced pulmonary innate immune and remodeling responses in mice.
      Authors: Kang MJ, Lee CG, Lee JY, Dela Cruz CS, Chen ZJ, Enelow R, Elias JA
      J. Clin. Invest., 2008;118(8):2771-84.
      Species: Mouse
      Sample Type: BALF
    99. CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals.
      Authors: Liu J, Yu Q, Stone GW, Yue FY, Ngai N, Jones RB, Kornbluth RS, Ostrowski MA
      Vaccine, 2008;26(32):4062-72.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    100. Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.
      Authors: Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
      J. Immunol., 2008;181(2):1491-8.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    101. Lycopene suppresses ovalbumin-induced airway inflammation in a murine model of asthma.
      Authors: Lee CM, Chang JH, Moon DO, Choi YH, Choi IW, Park YM, Kim GY
      Biochem. Biophys. Res. Commun., 2008;374(2):248-52.
      Species: Mouse
      Sample Type: BALF
    102. Curcumin attenuates ovalbumin-induced airway inflammation by regulating nitric oxide.
      Authors: Moon DO, Kim MO, Lee HJ, Choi YH, Park YM, Heo MS, Kim GY
      Biochem. Biophys. Res. Commun., 2008;375(2):275-9.
      Species: Mouse
      Sample Type: BALF
    103. The LTK63 adjuvant improves protection conferred by Ag85B DNA-protein prime-boosting vaccination against Mycobacterium tuberculosis infection by dampening IFN-gamma response.
      Authors: Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Fattorini L, Del Giudice G, Cassone A
      Vaccine, 2008;26(33):4237-43.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    104. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
      Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
      Eur. J. Pharmacol., 2008;590(1):400-8.
      Species: Mouse
      Sample Type: Tissue Homogenates
    105. GS143, an IkappaB ubiquitination inhibitor, inhibits allergic airway inflammation in mice.
      Authors: Hirose K, Wakashin H, Oki M, Kagami S, Suto A, Ikeda K, Watanabe N, Iwamoto I, Furuichi Y, Nakajima H
      Biochem. Biophys. Res. Commun., 2008;374(3):507-11.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    106. Salmonella choleraesuis as an anticancer agent in a syngeneic model of orthotopic hepatocellular carcinoma.
      Authors: Lee CH, Wu CL, Shiau AL
      Int. J. Cancer, 2008;122(4):930-5.
      Species: Mouse
      Sample Type: Tissue Homogenates
    107. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
      Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
      J. Exp. Med., 2008;205(9):2075-84.
      Species: Mouse
      Sample Type: Serum
    108. Crucial role of the interleukin-6/interleukin-17 cytokine axis in the induction of arthritis by glucose-6-phosphate isomerase.
      Authors: Iwanami K, Matsumoto I, Tanaka-Watanabe Y, Inoue A, Mihara M, Ohsugi Y, Mamura M, Goto D, Ito S, Tsutsumi A, Kishimoto T, Sumida T
      Arthritis Rheum., 2008;58(3):754-63.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    109. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
      Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
      Am. J. Pathol., 2008;172(5):1209-21.
      Species: Mouse
      Sample Type: Tissue Homogenates
    110. Thymic stromal lymphopoietin and thymic stromal lymphopoietin-conditioned dendritic cells induce regulatory T-cell differentiation and protection of NOD mice against diabetes.
      Authors: Besin G, Gaudreau S, Menard M, Guindi C, Dupuis G, Amrani A
      Diabetes, 2008;57(8):2107-17.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    111. Chemical sympathectomy increases susceptibility to ocular herpes simplex virus type 1 infection.
      Authors: Templeton A, Nguyen G, Ash JD, Straub RH, Carr DJ
      J. Neuroimmunol., 2008;197(1):37-46.
      Species: Mouse
      Sample Type: Tissue Homogenates
    112. Chronic intranasal administration of mould spores or extracts to unsensitized mice leads to lung allergic inflammation, hyper-reactivity and remodelling.
      Authors: Denis O, van den Brule S, Heymans J, Havaux X, Rochard C, Huaux F, Huygen K
      Immunology, 2007;122(2):268-78.
      Species: Mouse
      Sample Type: BALF
    113. Intercellular adhesion molecule-1 expression is required on multiple cell types for the development of experimental autoimmune encephalomyelitis.
      Authors: Bullard DC, Hu X, Schoeb TR, Collins RG, Beaudet AL, Barnum SR
      J. Immunol., 2007;178(2):851-7.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    114. Toll-like receptor 4 mediates the early inflammatory response after cold ischemia/reperfusion.
      Authors: Kaczorowski DJ, Nakao A, Mollen KP, Vallabhaneni R, Sugimoto R, Kohmoto J, Tobita K, Zuckerbraun BS, McCurry KR, Murase N, Billiar TR
      Transplantation, 2007;84(10):1279-87.
      Species: Mouse
      Sample Type: Serum
    115. IL-17 receptor signaling influences virus-induced corneal inflammation.
      Authors: Molesworth-Kenyon SJ, Yin R, Oakes JE, Lausch RN
      J. Leukoc. Biol., 2007;83(2):401-8.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    116. Prolonged inhaled allergen exposure can induce persistent tolerance.
      Authors: Van Hove CL, Maes T, Joos GF, Tournoy KG
      Am. J. Respir. Cell Mol. Biol., 2007;36(5):573-84.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    117. Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells.
      Authors: Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F
      Immunol. Lett., 2007;116(1):86-94.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    118. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity.
      Authors: Yamanouchi J, Rainbow D, Serra P, Howlett S, Hunter K, Garner VE, Gonzalez-Munoz A, Clark J, Veijola R, Cubbon R, Chen SL, Rosa R, Cumiskey AM, Serreze DV, Gregory S, Rogers J, Lyons PA, Healy B, Smink LJ, Todd JA, Peterson LB, Wicker LS, Santamaria P
      Nat. Genet., 2007;39(3):329-37.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    119. Granulocyte-macrophage colony-stimulating factor prevents diabetes development in NOD mice by inducing tolerogenic dendritic cells that sustain the suppressive function of CD4+CD25+ regulatory T cells.
      Authors: Gaudreau S, Guindi C, Menard M, Besin G, Dupuis G, Amrani A
      J. Immunol., 2007;179(6):3638-47.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    120. The endothelial cell-produced antiangiogenic cytokine vascular endothelial growth inhibitor induces dendritic cell maturation.
      Authors: Tian F, Grimaldo S, Fujita M, Fugita M, Cutts J, Vujanovic NL, Li LY
      J. Immunol., 2007;179(6):3742-51.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    121. Src homology 2 domain-containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung.
      Authors: Oh SY, Zheng T, Bailey ML, Barber DL, Schroeder JT, Kim YK, Zhu Z
      J. Allergy Clin. Immunol., 2007;119(1):123-31.
      Species: Mouse
      Sample Type: BALF
    122. Liposomal retinoic acids modulate asthma manifestations in mice.
      Authors: Maret M, Ruffie C, Periquet B, Campo AM, Menevret M, Phelep A, Dziewiszek K, Druilhe A, Pretolani M
      J. Nutr., 2007;137(12):2730-6.
      Species: Mouse
      Sample Type: BALF
    123. Suppression of established experimental autoimmune uveitis by anti-LFA-1alpha Ab.
      Authors: Ke Y, Sun D, Zhang P, Jiang G, Kaplan HJ, Shao H
      Invest. Ophthalmol. Vis. Sci., 2007;48(6):2667-75.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    124. Airway epithelial STAT3 is required for allergic inflammation in a murine model of asthma.
      Authors: Simeone-Penney MC, Severgnini M, Tu P, Homer RJ, Mariani TJ, Cohn L, Simon AR
      J. Immunol., 2007;178(10):6191-9.
      Species: Mouse
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    125. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
      Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
      Am. J. Pathol., 2007;170(4):1152-64.
      Species: Mouse
      Sample Type: Tissue Homogenates
    126. D-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma.
      Authors: Lee JS, Lee CM, Jeong YI, Jung ID, Kim BH, Seong EY, Kim JI, Choi IW, Chung HY, Park YM
      FEBS Lett., 2007;581(1):57-64.
      Species: Mouse
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    127. Potent induction of TNF-alpha during interaction of immune effectors with oral tumors as a potential mechanism for the loss of NK cell viability and function.
      Authors: Romero-Reyes M, Head C, Cacalano NA, Jewett A
      Apoptosis, 2007;12(11):2063-75.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    128. Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
      Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
      Blood, 2007;111(1):219-28.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    129. TLR2 is constitutively expressed within the kidney and participates in ischemic renal injury through both MyD88-dependent and -independent pathways.
      Authors: Shigeoka AA, Holscher TD, King AJ, Hall FW, Kiosses WB, Tobias PS, Mackman N, McKay DB
      J. Immunol., 2007;178(10):6252-8.
      Species: Mouse
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    130. IL-27 suppresses Th2 cell development and Th2 cytokines production from polarized Th2 cells: a novel therapeutic way for Th2-mediated allergic inflammation.
      Authors: Yoshimoto T, Yoshimoto T, Yasuda K, Mizuguchi J, Nakanishi K
      J. Immunol., 2007;179(7):4415-23.
      Species: Mouse
      Sample Type: Serum
    131. Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
      Authors: Yuasa K, Yoshimura M, Urasawa N, Ohshima S, Howell JM, Nakamura A, Hijikata T, Miyagoe-Suzuki Y, Takeda S
      Gene Ther., 2007;14(17):1249-60.
      Species: Mouse
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    132. Maternal immune activation alters fetal brain development through interleukin-6.
      Authors: Smith SE, Li J, Garbett K, Mirnics K, Patterson PH
      J. Neurosci., 2007;27(40):10695-702.
      Species: Mouse
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    133. Oral administration of an immunostimulatory DNA sequence from Bifidobacterium longum improves Th1/Th2 balance in a murine model.
      Authors: Takahashi N, Kitazawa H, Iwabuchi N, Xiao JZ, Miyaji K, Iwatsuki K, Saito T
      Biosci. Biotechnol. Biochem., 2006;70(8):2013-7.
      Species: Mouse
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    134. Induction of innate immunity against herpes simplex virus type 2 infection via local delivery of Toll-like receptor ligands correlates with beta interferon production.
      Authors: Gill N, Deacon PM, Lichty B, Mossman KL, Ashkar AA
      J. Virol., 2006;80(20):9943-50.
      Species: Mouse
      Sample Type: Vaginal Lavage
    135. A multivalent combination of experimental antituberculosis DNA vaccines based on Ag85B and regions of difference antigens.
      Authors: Grover A, Ahmed MF, Singh B, Verma I, Sharma P, Khuller GK
      Microbes Infect., 2006;8(9):2390-9.
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    136. Roles of the small intestine for induction of toll-like receptor 4-mediated innate resistance in naturally acquired murine toxoplasmosis.
      Authors: Furuta T, Yoshikawa Y
      Int. Immunol., 2006;18(12):1655-62.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    137. Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation.
      Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X
      Biochem. Biophys. Res. Commun., 2006;345(3):1122-30.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    138. The differential effects of low dose and high dose concanavalin A on cytokine profile and their importance in liver injury.
      Authors: Xu X, Wei H, Dong Z, Chen Y, Tian Z
      Inflamm. Res., 2006;55(4):144-52.
      Species: Mouse
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    139. Vaccinia virus infection attenuates innate immune responses and antigen presentation by epidermal dendritic cells.
      Authors: Deng L, Dai P, Ding W, Granstein RD, Shuman S
      J. Virol., 2006;80(20):9977-87.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    140. Blockade of transforming growth factor-{beta} signaling in tumor-reactive CD8(+) T cells activates the antitumor immune response cycle.
      Authors: Zhang Q, Yang XJ, Kundu SD, Pins M, Javonovic B, Meyer R, Kim SJ, Greenberg NM, Kuzel T, Meagher R, Guo Y, Lee C
      Mol. Cancer Ther., 2006;5(7):1733-43.
      Species: Mouse
      Sample Type: Serum
    141. Protease-activated receptor-2 activation: a major role in the pathogenesis of Porphyromonas gingivalis infection.
      Authors: Holzhausen M, Spolidorio LC, Ellen RP, Jobin MC, Steinhoff M, Andrade-Gordon P, Vergnolle N
      Am. J. Pathol., 2006;168(4):1189-99.
      Species: Mouse
      Sample Type: Blister Fluid
    142. Perforin-deficient CD8+ T cells mediate fatal lymphocytic choriomeningitis despite impaired cytokine production.
      Authors: Storm P, Bartholdy C, Sorensen MR, Christensen JP, Thomsen AR
      J. Virol., 2006;80(3):1222-30.
      Species: Mouse
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    143. Prevention of murine experimental autoimmune encephalomyelitis by in vivo expression of a novel recombinant immunotoxin DT390-RANTES.
      Authors: Jia Y, Li H, Chen W, Li M, Lv M, Feng P, Hu H, Zhang L
      Gene Ther., 2006;13(18):1351-9.
      Species: Mouse
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    144. CD27 contributes to the early systemic immune response to Mycobacterium tuberculosis infection but does not affect outcome.
      Authors: Wieland CW, Kerver ME, Florquin S, Nolte MA, Borst J, van Lier R, van Oers MH, van der Poll T
      Int. Immunol., 2006;18(11):1531-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    145. Type I IFN modulates host defense and late hyperinflammation in septic peritonitis.
      Authors: Weighardt H, Kaiser-Moore S, Schlautkotter S, Rossmann-Bloeck T, Schleicher U, Bogdan C, Holzmann B
      J. Immunol., 2006;177(8):5623-30.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    146. Contribution of T cell subsets to the pathophysiology of Pneumocystis-related immunorestitution disease.
      Authors: Bhagwat SP, Gigliotti F, Xu H, Wright TW
      Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(6):L1256-66.
      Species: Mouse
      Sample Type: BALF
    147. Short-term dietary administration of celecoxib enhances the efficacy of tumor lysate-pulsed dendritic cell vaccines in treating murine breast cancer.
      Authors: Hahn T, Alvarez I, Kobie JJ, Ramanathapuram L, Dial S, Fulton A, Besselsen D, Walker E, Akporiaye ET
      Int. J. Cancer, 2006;118(9):2220-31.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    148. CCL17 transgenic mice show an enhanced Th2-type response to both allergic and non-allergic stimuli.
      Authors: Tsunemi Y, Saeki H, Nakamura K, Nagakubo D, Nakayama T, Yoshie O, Kagami S, Shimazu K, Kadono T, Sugaya M, Komine M, Matsushima K, Tamaki K
      Eur. J. Immunol., 2006;36(8):2116-27.
      Species: Mouse
      Sample Type: Tissue Homogenates
    149. Inhalation tolerance is induced selectively in thoracic lymph nodes but executed pervasively at distant mucosal and nonmucosal tissues.
      Authors: Alvarez D, Swirski FK, Yang TC, Fattouh R, Croitoru K, Bramson JL, Stampfli MR, Jordana M
      J. Immunol., 2006;176(4):2568-80.
      Species: Mouse
      Sample Type: Serum
    150. Endogenous interleukin-12 improves the early antimicrobial host response to murine Escherichia coli peritonitis.
      Authors: Weijer S, Florquin S, van der Poll T
      Shock, 2005;23(1):54-8.
      Species: Mouse
      Sample Type: Peritoneal Fluid
    151. Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
      Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
      Vaccine, 2005;24(9):1470-7.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    152. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
      Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
      J. Immunol., 2005;175(4):2589-97.
      Species: Mouse
      Sample Type: BALF
    153. Vaccination of apoptotic Fas ligand-expressing tumors decreased antitumor responses by enhanced production of immunosuppressive cytokines.
      Authors: Wada A, Tada Y, Shimozato O, Takiguchi Y, Tatsumi K, Kuriyama T, Tagawa M
      Anticancer Res., 2005;25(1):299-303.
      Species: Mouse
      Sample Type: Tissue Secretion
    154. Inhaled p38alpha mitogen-activated protein kinase antisense oligonucleotide attenuates asthma in mice.
      Authors: Duan W, Chan JH, McKay K, Crosby JR, Choo HH, Leung BP, Karras JG, Wong WS
      Am. J. Respir. Crit. Care Med., 2005;171(6):571-8.
      Species: Mouse
      Sample Type: BALF
    155. Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
      Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
      Life Sci., 2005;78(9):987-94.
      Species: Mouse
      Sample Type: BALF
    156. DNA vaccine encoding Der p 2 allergen generates immunologic protection in recombinant Der p 2 allergen-induced allergic airway inflammation mice model.
      Authors: Li GP, Liu ZG, Ran PX, Zhong NS
      Chin. Med. J., 2005;118(7):534-40.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    157. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
      Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
      Comp. Immunol. Microbiol. Infect. Dis., 2004;27(6):393-411.
      Species: Mouse
      Sample Type: Serum
    158. Vbeta14(+) T cells mediate the vaccine-enhanced disease induced by immunization with respiratory syncytial virus (RSV) G glycoprotein but not with formalin-inactivated RSV.
      Authors: Johnson TR, Varga SM, Braciale TJ, Graham BS
      J. Virol., 2004;78(16):8753-60.
      Species: Mouse
      Sample Type: Tissue Homogenates
    159. Cytokine expression in experimental chronic otitis media with effusion in mice.
      Authors: Maeda K, Hirano T, Ichimiya I, Kurono Y, Suzuki M, Mogi G
      Laryngoscope, 2004;114(11):1967-72.
      Species: Mouse
      Sample Type: Tissue Secretion
    160. Control of Leishmania major in the absence of Tyk2 kinase.
      Authors: Schleicher U, Mattner J, Blos M, Schindler H, Rollinghoff M, Karaghiosoff M, Muller M, Werner-Felmayer G, Bogdan C
      Eur. J. Immunol., 2004;34(2):519-29.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    161. IL-22 increases the innate immunity of tissues.
      Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
      Immunity, 2004;21(2):241-54.
      Species: Mouse
      Sample Type: Plasma
    162. Increased and long-term generation of dendritic cells with reduced function from IL-6-deficient bone marrow.
      Authors: Bleier JI, Pillarisetty VG, Shah AB, DeMatteo RP
      J. Immunol., 2004;172(12):7408-16.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    163. In vivo effects of interleukin-17 on haematopoietic cells and cytokine release in normal mice.
      Authors: Jovcic G, Bugarski D, Petakov M, Krstic A, Vlaski M, Stojanovic N, Milenkovic P
      Cell Prolif., 2004;37(6):401-12.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    164. Suppression of graft coronary artery disease by a brief treatment with a selective epsilonPKC activator and a deltaPKC inhibitor in murine cardiac allografts.
      Authors: Tanaka M, Terry RD, Mokhtari GK, Inagaki K, Koyanagi T, Kofidis T, Mochly-Rosen D, Robbins RC
      Circulation, 2004;110(11):II194-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    165. Single intranasal mucosal Mycobacterium bovis BCG vaccination confers improved protection compared to subcutaneous vaccination against pulmonary tuberculosis.
      Authors: Chen L, Wang J, Zganiacz A, Xing Z
      Infect. Immun., 2004;72(1):238-46.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    166. Dual roles of IL-4 in lung injury and fibrosis.
      Authors: Huaux F, Liu T, McGarry B, Ullenbruch M, Phan SH
      J. Immunol., 2003;170(4):2083-92.
      Species: Mouse
      Sample Type: Serum
    167. A protective role of locally administered immunostimulatory CpG oligodeoxynucleotide in a mouse model of genital herpes infection.
      Authors: Harandi AM, Eriksson K, Holmgren J
      J. Virol., 2003;77(2):953-62.
      Species: Mouse
      Sample Type: Serum
    168. MHC class II-associated invariant chain isoforms regulate pulmonary immune responses.
      Authors: Ye Q, Finn PW, Sweeney R, Bikoff EK, Riese RJ
      J. Immunol., 2003;170(3):1473-80.
      Species: Human
      Sample Type: BALF
    169. Administration of either anti-CD40 or interleukin-12 following lethal total body irradiation induces acute lethal toxicity affecting the gut.
      Authors: Hixon JA, Anver MR, Blazar BR, Panoskaltsis-Mortari A, Wiltrout RH, Murphy WJ
      Biol. Blood Marrow Transplant., 2002;8(6):316-25.
      Species: Mouse
      Sample Type: Serum
    170. IFN-gamma, but not Fas, mediates reduction of allergen-induced mucous cell metaplasia by inducing apoptosis.
      Authors: Shi ZO, Fischer MJ, De Sanctis GT, Schuyler MR, Tesfaigzi Y
      J. Immunol., 2002;168(9):4764-71.
    171. Endogenous granulocyte-macrophage colony-stimulating factor overexpression in vivo results in the long-term recruitment of a distinct dendritic cell population with enhanced immunostimulatory function.
      Authors: Miller G, Pillarisetty VG, Shah AB, Lahrs S, Xing Z, DeMatteo RP
      J. Immunol., 2002;169(6):2875-85.
      Species: Mouse
      Sample Type: Serum
    172. Leptin accelerates autoimmune diabetes in female NOD mice.
      Authors: Matarese G, Sanna V, Lechler RI, Sarvetnick N, Fontana S, Zappacosta S, La Cava A
      Diabetes, 2002;51(5):1356-61.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    173. Anti-tumor mechanism of Z-100, an immunomodulatory Arabinomannan extracted from Mycobacterium tuberculosis strain Aoyama B, on pulmonary metastases of B16F10 melanoma: restoration of helper T cell responses via suppression of glucocorticoid-genesis.
      Authors: Oka H, Emori Y, Sasaki H, Shiraishi Y, Yoshinaga K, Kurimoto T
      Microbiol. Immunol., 2002;46(5):343-51.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    174. A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
      Authors: Huaux F, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
      J. Immunol., 2002;169(5):2653-61.
      Species: Mouse
      Sample Type: Tissue Homogenates
    175. STAT4 serine phosphorylation is critical for IL-12-induced IFN-gamma production but not for cell proliferation.
      Authors: O&amp;amp;apos;shea JJ
      Proc. Natl. Acad. Sci. U.S.A., 2002;99(19):12281-6.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    176. Neutrophil-mediated mycobacteriocidal immunity in the lung during Mycobacterium bovis BCG infection in C57BL/6 mice.
      Authors: Fulton SA, Reba SM, Martin TD, Boom WH
      Infect. Immun., 2002;70(9):5322-7.
      Species: Mouse
      Sample Type: BALF
    177. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
      Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
      Eur. J. Immunol., 2001;31(7):2104-15.
      Species: Mouse
      Sample Type: Cell Culture Supernates
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    Sample TestedCell culture supernatant
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    Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
     Mouse IFN-gamma Quantikine ELISA Kit MIF00
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     Mouse IFN-gamma Quantikine ELISA Kit MIF00
    : Mouse IFN-gamma Quantikine ELISA Kit [MIF00]

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    Sample TestedMouse primary CD8 cells

    Other Experimental Details

    Other Experimental DetailsAdditional washes were added. Control or drug-treated CD8 cells were treated with allogeneic APC for 24 or 48 hours. Supernatants were subjected to IFN-g ELISA per manufacture's recommendation.

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