Mouse IGFBP-3 Antibody

Catalog # Availability Size / Price Qty
MAB775
MAB775-SP
Product Details
Citations (4)
FAQs
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Mouse IGFBP-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGFBP-3 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human IGFBP‑1, rhIGFBP‑2, rhIGFBP‑3, rhIGFBP‑4, rhIGFBP‑5, rhIGFBP‑6, recombinant mouse (rm) IGFBP‑2, rmIGFBP‑5, or rmIGFBP‑6.
Source
Monoclonal Rat IgG2B Clone # 138202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFBP‑3
Pro22-Gln291 (Arg250Gln, Gln259Arg, and Arg271Pro)
Accession # P47878
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IGFBP‑3 (Catalog # 775-B3)
under non-reducing conditions only

Mouse IGFBP-3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IGFBP‑3 Biotinylated Antibody (Catalog # BAF775)

Standard: Recombinant Mouse IGFBP-3 Protein, CF (Catalog # 775-B3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-3

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked glycosylation sites. Mouse and human IGFBP-3 share 81% amino acid homology. Mouse IGFBP-3 is expressed in multiple tissues.

References
  1. Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
Long Name
Insulin-like Growth Factor Binding Protein 3
Entrez Gene IDs
3486 (Human); 16009 (Mouse)
Alternate Names
acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3

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Citations for Mouse IGFBP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. IGFBP-3 is a metastasis suppression gene in prostate cancer.
    Authors: Mehta HH, Gao Q, Galet C
    Cancer Res., 2011-06-22;71(15):5154-63.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  2. Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation.
    Authors: Yamada PM, Mehta HH, Hwang D
    Endocrinology, 2010-10-06;151(12):5741-50.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  3. Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model.
    Authors: Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R
    Cancer Res., 2007-11-15;67(22):11083-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  4. The ternary IGF complex influences postnatal bone acquisition and the skeletal response to intermittent parathyroid hormone.
    Authors: Yakar S, Bouxsein ML, Canalis E, Sun H, Glatt V, Gundberg C, Cohen P, Hwang D, Boisclair Y, LeRoith D, Rosen CJ
    J. Endocrinol., 2006-05-01;189(2):289-99.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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