Detects mouse IGFBP-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IGFBP-3 is observed and less than 1% cross-reactivity with rhIGFBP-1, rhIGFBP-2, rhIGFBP-4, rhIGFBP‑5, rhIGFBP‑6, and recombinant mouse IGFBP‑6 is observed.
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IGFBP‑3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for IGFBP‑3 at approximately 41 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse IGFBP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF775) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.
Mouse IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked glycosylation sites. Mouse and human IGFBP-3 share 81% amino acid homology. Mouse IGFBP-3 is expressed in multiple tissues.
Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
Insulin-like Growth Factor Binding Protein 3
Entrez Gene IDs:
3486 (Human); 16009 (Mouse)
acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IBP-3; IBP3BP-53; IGF-binding protein 3; IGFBP3; IGFBP-3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3
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