IGFBP-3 (Insulin-like growth factor binding protein-3) modulates the biological activity of IGF. It circulates in a ternary complex with IGF and the acid-labile subunit (ALS). Proteolysis of IGFBP-3 by tissue plasminogen activator (tPA), a disintegrin and metalloproteases (ADAMs), and prostate specific antigen (PSA) contributes to IGFBP-3 degradation or a reduction in its affinity for IGF. The majority of soluble IGFBP-3 found in circulation is secreted from hepatic non-parenchymal cells. IGFBP-3 potentiates EGF-EGFR-mediated cell growth, modulates adipogenesis, and regulates TGF-beta signaling, DNA damage, apoptosis, autophagy, and gene transcription.
Mouse IGFBP-3 Quantikine ELISA Kit
R&D Systems | Catalog # MGB300
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), EDTA Plasma (10 µL)
Sensitivity
16 pg/mL
Assay Range
78.1-5000 pg/mL (Cell Culture Supernates, EDTA Plasma)
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Product Summary for Mouse IGFBP-3 Quantikine ELISA Kit
The Quantikine Mouse IGFBP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IGFBP-3 in cell culture supernates and plasma. It contains NS0-expressed recombinant mouse IGFBP-3 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IGFBP-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IGFBP-3.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse IGFBP-3. This kit detects full-length and fragmented mouse IGFBP-3. It also detects IGF-I, IGF-II, IGF-I/ALS, and IGF-II/ALS complexed IGFBP-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 280 | 628 | 2653 | 283 | 602 | 2648 |
| Standard Deviation | 13.5 | 25.1 | 148 | 23.4 | 44.4 | 156 |
| CV% | 4.8 | 4.0 | 5.6 | 8.3 | 7.4 | 5.9 |
Recovery for Mouse IGFBP-3 Quantikine ELISA Kit
The recovery of mouse IGFBP-3 spiked to three levels throughout the range of the assay in the cell culture supernate samples was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 100 | 90-109 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse IGFBP-3 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse IGFBP-3 Quantikine ELISA Kit
Mouse IGFBP-3 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGFBP-3
Long Name
Insulin-like Growth Factor Binding Protein 3
Alternate Names
IGFBP3
Gene Symbol
IGFBP3
Additional IGFBP-3 Products
Product Documents for Mouse IGFBP-3 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IGFBP-3 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse IGFBP-3 Quantikine ELISA Kit
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Protocols
View specific protocols for Mouse IGFBP-3 Quantikine ELISA Kit (MGB300):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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