Mouse IGFBP-6 Antibody

Catalog # Availability Size / Price Qty
MAB7761
MAB7761-SP
Product Details
FAQs
Supplemental Products
Reviews

Mouse IGFBP-6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGFBP-6 in ELISAs and Western blots. In Western blots, 10% cross-reactivity with recombinant human (rh) IGFBP-3 and rhIGFBP-5 is observed and no cross-reactivity with rhIGFBP-1, recombinant mouse (rm) IGFBP-1, rhIGFBP-2, rmIGFBP-2, rmIGFBP-3, rhIGFBP-4, rmIGFBP-5, or rhIGFBP-6 is observed.
Source
Monoclonal Rat IgG2A Clone # 122312
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-6
Gly24-Gly238
Accession # P47880
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IGFBP‑6 (Catalog # 776-B6) under non-reducing conditions only

Mouse IGFBP-6 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IGFBP‑6 Biotinylated Antibody (Catalog # BAF776)

Standard: Recombinant Mouse IGFBP-6 Protein, CF (Catalog # 776-B6)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-6

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-6 cDNA encodes a 238 amino acid (aa) precursor protein with a putative 25 aa signal peptide that is processed to generate the 213 aa mature protein that is O-glycosylated. Mouse and human IGFBP-6 share 73% aa similarity. Mouse and rat IGFBP-6 share 94% aa similarity and the mouse IGFBP-6 has a 9 amino acid insertion compared to the rat homolog. IGFBP-6 is expressed in ovarian, testicular, muscle, heart and lung tissues in the adult mouse. IGFBP-6 was not detected in total RNA from a whole mouse embryo.

References
  1. Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
  2. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  3. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Long Name
Insulin-like Growth Factor Binding Protein 6
Entrez Gene IDs
3489 (Human); 16012 (Mouse)
Alternate Names
IBP6; IBP-6; IGF binding protein 6; IGF-binding protein 6; IGFBP6; IGFBP-6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse IGFBP-6 Antibody

There are currently no reviews for this product. Be the first to review Mouse IGFBP-6 Antibody and earn rewards!

Have you used Mouse IGFBP-6 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review