Mouse IGFBP-6 Antibody

Catalog # Availability Size / Price Qty
AF776
AF776-SP
Product Details
Citations (1)
FAQs
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Mouse IGFBP-6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGFBP-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) IGFBP-6 is observed and less than 1% cross-reactivity with rhIGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4, and rhIGFBP-5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-6
Gly24-Gly238
Accession # P47880
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IGFBP‑6 (Catalog # 776-B6)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse ovary

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-6

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-6 cDNA encodes a 238 amino acid (aa) residue precursor protein with a putative 25 aa residue signal peptide that is processed to generate the 213 aa residue mature protein that is O-glycosylated. Mouse and human IGFBP-6 share 73% amino acid similarity. Mouse and rat IGFBP-6 share 94% amino acid similarity and the mouse IGFBP-6 has a 9 amino acid insertion compared to the rat homolog. IGFBP-6 is expressed in ovarian, testicular, muscle, heart and lung tissues in the adult mouse. IGFBP-6 was not detected in total RNA from a whole mouse embryo.

References
  1. Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
  2. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  3. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Long Name
Insulin-like Growth Factor Binding Protein 6
Entrez Gene IDs
3489 (Human); 16012 (Mouse)
Alternate Names
IBP6; IBP-6; IGF binding protein 6; IGF-binding protein 6; IGFBP6; IGFBP-6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6

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Citation for Mouse IGFBP-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Myelin formation during development of the CNS is delayed in matrix metalloproteinase-9 and -12 null mice.
    Authors: Larsen PH, DaSilva AG, Conant K, Yong VW
    J. Neurosci., 2006-02-22;26(8):2207-14.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot

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