Human ULBP-2/5/6 Antibody

(34 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.
  • Source
    Monoclonal Mouse IgG2A Clone # 165903
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human ULBP-2
    Accession # Q9BZM5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.03 - 0.1 μg/mL of this antibody will block 50% of the binding of 25 ng/mL of biotinylated rhULBP-2/Fc to immobilized rhNKG2D/Fc chimera coated at 2 μg/mL (100 μL/well). At 1 μg/mL, this antibody will achieve 85% blocking in the assay. This antibody will block >70% of the binding of either Recombinant Human ULBP-5 Fc Chimera or Recombinant ULBP-6 Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ULBP-2/5/6
ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.
  • References:
    1. Cosman, D. et al. (2001) Immunity 14:123.
    2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
    3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
    4. Steinle, A. et al. (2001) Immunogenetics 53:279.
    5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
    6. Pende, D. et al. (2002) Cancer Res. 62:6178.
    7. Radosavljevic, M. et al. (2002) Genomics 79:114.
    8. NKG2D and its Ligands (2002) www.RnDSystems.com.
  • Long Name:
    UL16 Binding Protein-2/5/6
  • Alternate Names:
    ULBP-2/5/6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Adoptively transferred V?9V?2 T cells show potent antitumor effects in a preclinical B cell lymphomagenesis model
    Authors: NA Zumwalde, A Sharma, X Xu, S Ma, CL Schneider, JC Romero-Mas, AW Hudson, A Gendron-Fi, SC Kenney, JE Gumperz
    JCI Insight, 2017;2(13):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
    Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
    Oncotarget, 2017;8(19):31959-31976.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Control of Tumor Initiation by NKG2D Naturally Expressed on Ovarian Cancer Cells
    Authors: X Cai, A Caballero-, MM Gewe, IC Jenkins, CW Drescher, RK Strong, T Spies, V Groh
    Neoplasia, 2017;19(6):471-482.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  4. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
    Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
    Sci Rep, 2017;7(1):8661.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells
    Sci Rep, 2016;6(0):32470.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
    Authors: Kim, Ga Rim, Ha, Ga-Hee, Bae, Jae-Ho, Oh, Sae-Ock, Kim, Sun-Hee, Kang, Chi-Dug
    Oncol Lett, 2015;9(4):1641-1646.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
    Authors: Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A
    Eur J Immunol, 2015;45(8):2324-34.
    Species: Human
    Sample Type: Plasma
    Application: IP
  8. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
    Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
    BMC Cancer, 2015;15(0):351.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Methylation of NKG2D ligands contributes to immune system evasion in acute myeloid leukemia.
    Authors: Baragano Raneros A, Martin-Palanco V, Fernandez A, Rodriguez R, Fraga M, Lopez-Larrea C, Suarez-Alvarez B
    Genes Immun, 2015;16(1):71-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. PRL-3 mediates the protein maturation of ULBP2 by regulating the tyrosine phosphorylation of HSP60.
    Authors: Leung W, Vong Q, Lin W, Bouck D, Wendt S, Sullivan E, Li Y, Bari R, Chen T, Leung W
    J Immunol, 2015;194(6):2930-41.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA detection
  11. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
    J Immunol, 2014;192(11):5332-42.
    Species: Human
    Sample Type: Whole Blood
    Application: Neut
  12. Natural killer cell-mediated shedding of ULBP2.
    Authors: Wang, Ruipeng, Sun, Peter D
    PLoS ONE, 2014;9(3):e91133.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
    Authors: Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi M, Petrucci M, Cippitelli M, Santoni A
    J Immunol, 2014;193(2):950-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy.
    Authors: Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, Choi J, Choi I, Yun C, Kim J, Yee C, Kumar V, Lee K
    Cancer Res, 2013;73(8):2598-607.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation.
    Authors: Vacca P, Martini S, Garelli V, Passalacqua G, Moretta L, Mingari M
    Eur J Immunol, 2013;43(2):550-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells.
    Authors: Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
    Cancer Res, 2012;72(21):5463-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012;8(1):e1002486.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012;7(2):e31959.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
    Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
    Lab. Invest., 2012;92(7):967-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
    Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
    Oncogene, 2011;30(23):2622-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. Human adipose-derived stem cells impair natural killer cell function and exhibit low susceptibility to natural killer-mediated lysis.
    Authors: Delarosa O, Sanchez-Correa B, Morgado S, Ramirez C, del Rio B, Menta R, Lombardo E, Tarazona R, Casado JG
    Stem Cells Dev., 2011;21(8):1333-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  22. The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.
    Authors: Fernandez-Messina L, Ashiru O, Aguera-Gonzalez S, Reyburn HT, Vales-Gomez M
    J. Cell. Sci., 2011;124(0):321-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  23. Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
    Authors: Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M
    Blood, 2010;115(25):5170-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  24. The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
    Authors: Nuckel H, Switala M, Sellmann L, Horn PA, Durig J, Duhrsen U, Kuppers R, Grosse-Wilde H, Rebmann V
    Leukemia, 2010;24(6):1152-9.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  25. Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
    Authors: Ohashi M, Eagle RA, Trowsdale J
    J. Biol. Chem., 2010;285(22):16408-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  26. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009;113(13):2955-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  27. Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation.
    Authors: Ho VT, Vanneman M, Kim H, Sasada T, Kang YJ, Pasek M, Cutler C, Koreth J, Alyea E, Sarantopoulos S, Antin JH, Ritz J, Canning C, Kutok J, Mihm MC, Dranoff G, Soiffer R
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(37):15825-30.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  28. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  29. Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
    Authors: Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A
    Int. J. Cancer, 2009;124(7):1594-604.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  30. Melanoma-associated fibroblasts modulate NK cell phenotype and antitumor cytotoxicity.
    Authors: Balsamo M, Scordamaglia F, Pietra G, Manzini C, Cantoni C, Boitano M, Queirolo P, Vermi W, Facchetti F, Moretta A, Moretta L, Mingari MC, Vitale M
    Proc. Natl. Acad. Sci. U.S.A., 2009;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  31. Effective in vivo induction of NKG2D ligands in acute myeloid leukaemias by all-trans-retinoic acid or sodium valproate.
    Authors: Poggi A, Catellani S, Garuti A, Pierri I, Gobbi M, Zocchi MR
    Leukemia, 2009;23(4):641-8.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  32. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
    Authors: Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
    Mol. Immunol., 2008;45(15):3917-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  33. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007;111(3):1309-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  34. HIV modulates the expression of ligands important in triggering natural killer cell cytotoxic responses on infected primary T-cell blasts.
    Authors: Ward J, Bonaparte M, Sacks J, Guterman J, Fogli M, Mavilio D, Barker E
    Blood, 2007;110(4):1207-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 34 Citations
Isotype Controls
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Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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