Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

Catalog # Availability Size / Price Qty
MHSLB00
Control Products Available
Mouse IL-1 beta /IL-1F2 Standard Curve
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Product Details
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Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.312 pg/mL
Assay Range
0.8 - 50 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® HS Mouse IL-1 beta /IL-1F2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse IL-1 beta levels in serum and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant protein. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine® HS kit standards. These results indicate that the Quantikine® HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-1 beta.

Precision

Intra-Assay Precision (Precision within an assay) <div>Three samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) <div>Three samples of known concentration were tested in twenty separate assays to assess&nbsp;inter-assay precision. Assays were performed by at least three technicians.</div>

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 4.64 14.1 28.5 4.89 14.5 28.9
Standard Deviation 0.13 0.378 0.59 0.413 0.796 1.6
CV% 2.8 2.7 2.1 8.4 5.5 5.5

Recovery

The recovery of mouse IL-1 beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 98 91-105
Heparin Plasma (n=4) 92 84-99
Serum (n=4) 95 87-101

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-1 beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse IL-1 beta /IL-1F2 Linearity

Data Examples

Mouse IL-1 beta /IL-1F2 Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

FAQs

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Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit
By Anonymous on 09/14/2019
Sample Tested: Serum and Plasma