Mouse IL-17 RD/SEF Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL‑17 RD/SEF in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 400210
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-17 RD/SEF
    Gly28-Arg299
    Accession # Q8JZL1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
IL‑17 RD/SEF in Mouse Liver. IL‑17 RD/SEF was detected in perfusion fixed frozen sections of mouse liver using 25 µg/mL Mouse IL‑17 RD/SEF Monoclonal Antibody (Catalog # MAB2276) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
IL‑17 RD/SEF in Mouse Thymus. IL‑17 RD/SEF was detected in perfusion fixed frozen sections of mouse thymus using Mouse IL‑17 RD/SEF Monoclonal Antibody (Catalog # MAB2276) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
IL‑17 RD/SEF in Mouse Lung. IL‑17 RD/SEF was detected in immersion fixed frozen sections of mouse lung using Mouse IL‑17 RD/SEF Monoclonal Antibody (Catalog # MAB2276) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17 RD/SEF

Interleukin-17 receptor D (IL-17 RD), also known as SEF (similar expression to FGFs), is a type I transmembrane protein that is found in both the cytoplasm and plasma membrane (1‑5). The gene for this protein belongs to a synexpression group originally identified in zebrafish and SEF is expressed along with FGF-3, -8, sprouty-2 (SPRY2) and SPRY4 (6, 7). Due to the presence of an alternate start site, there is one transcript that potentially gives rise to two isoforms. The first is a full-length long form and the second an N-terminally truncated form (2, 5). The significance and expression pattern of the short form are uncertain. The membrane‑bound long form of mouse IL-17 RD is synthesized as a 738 amino acid (aa) precursor protein with a putative 27 aa signal peptide, a 272 aa extracellular domain, a 20 aa transmembrane segment and a 419 aa cytoplastic domain (5). The extracellular domain contains one Ig-like domain and a fibronectin type III motif. The cytoplasmic domain shares homology with the intracellular domains of IL‑17 receptor family members and shows one TIR (Toll/IL-1 Receptor) domain and a putative TRAF6-binding motif (2). Natural IL-17 RD has been shown to form homomultimeric complexes (3). The full-length IL-17 RD isoform is expressed in most adult tissues and during embryonic development (3, 5). Functionally, IL-17 RD has been shown to be an inhibitor of FGF signaling. The molecule’s extracellular domain does not seem to be involved. There is an interaction between the intracellular domains of FGF R1/2 and IL-17 RD that blocks ERK dissociation from MEK, thereby interfering with downstream ERK activation of nuclear Elk-1 (8). IL-17 RD has also been reported to interact with TAK1 and induce JNK activation and apoptosis (9). Ligands that interact with the extracellular domain of IL-17 RD have not been identified.

  • References:
    1. Furthauer, M. et al. (2002) Nat. Cell Biol. 4:170.
    2. Xiong, S. et al. (2003) J. Biol. Chem. 278:50273.
    3. Yang, R-B. et al. (2003) J. Biol. Chem. 278:33232.
    4. Preger, E. et al. (2003) Proc. Natl. Acad. Sci. USA 101:1229.
    5. Lin, W. et al. (2002) Mech. Dev. 113:163.
    6. Tsang, M. et al. (2002) Nat. Cell Biol. 4:165.
    7. Kovalenko, D. et al. (2003) J. Biol. Chem. 278:14087.
    8. Torii, S. et al. (2004) Dev. Cell 7:33.
    9. Yang, X. et al. (2004) J. Biol. Chem. 279:38099.
  • Long Name:
    Interleukin 17 Receptor D, Transcript Variant 3
  • Entrez Gene IDs:
    54756 (Human); 171463 (Mouse)
  • Alternate Names:
    FLJ35755; IL-17 RD; IL17RD; IL-17RD; IL-17RDIL-17 receptor D; IL17Rhom; IL17RLM; IL17RLMDKFZp434N1928; interleukin 17 receptor D; interleukin-17 receptor D; Interleukin-17 receptor-like protein; MGC133309; Sef homolog; SEF; SEFhSef
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Protective role for TLR4 signaling in atherosclerosis progression as revealed by infection with a common oral pathogen.
    Authors: Hayashi C, Papadopoulos G, Gudino C, Weinberg E, Barth K, Madrigal A, Chen Y, Ning H, LaValley M, Gibson F, Hamilton J, Genco C
    J Immunol, 2012;189(7):3681-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Cell and Tissue Staining Kits
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Anti-Rat HRP-DAB Cell & Tissue Staining Kit

CTS017 5
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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