Mouse IL-17RB Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1040
Product Details
Citations (5)
FAQs
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Mouse IL-17RB Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑17 RB in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human IL‑17 RB, recombinant mouse (rm) IL-17 and rmIL-17 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RB
Arg18-Gly286
Accession # Q9JIP3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑17 RB Fc Chimera (Catalog # 1040-BR)

Mouse IL-17 RB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IL-17RB Antibody (Catalog # MAB10401)

Standard: Recombinant Mouse IL-17RB Fc Chimera Protein, CF (Catalog # 1040-BR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine production and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1, 2). Interleukin-17B Receptor (IL-17RB), also known as IL-17 Rh1, IL-17E R and EVI27, represents the second receptor of the IL-17 family cytokines to be recognized (2‑4). Mouse IL17B R cDNA encodes a 499 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 269 aa extracellular domain, a 21 aa transmembrane domain and a 192 aa cytoplasmic tail. As a result of alternative splicing, a secreted variant of IL-17B R also exists (4). Human and mouse IL‑17B R share 76% aa sequence identity. The IL-17B R protein sequence is only 19.2% and 18.2% identical to the human and mouse IL-17 R sequence, respectively. However, the receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. At least three additional type I transmembrane receptors with homology to IL‑17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have been reported (2, 6). By Northern blot analysis, mouse IL-17B R is highly expressed in liver and testes and is expressed at lower levels in kidney and lung. It is also expressed in some hematopoietic cell lines, including selected T cell, B cell, and myeloid cell lines (2‑4). The expression of IL-17B R is significantly up‑regulated under inflammatory conditions (7). IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17 or IL-17F. Activation of IL-17B R by its ligands results in the activation of nuclear factor kappa-B (2‑4).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
  4. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  5. Tian, E. et al. (2000) Oncogene 19:2098.
  6. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  7. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Long Name
Interleukin 17B Receptor
Entrez Gene IDs
55540 (Human); 50905 (Mouse)
Alternate Names
CRL4IL-17 receptor homolog 1; cytokine receptor CRL4; Cytokine receptor-like 4; Evi27; EVI27IL-17B receptor; IL-17 RB; IL-17 receptor B; IL-17 RH1; IL17BRinterleukin 17B receptor; IL-17ER; IL17RB; IL-17RB; IL17Rh1; IL-17Rh1; IL17RH1MGC5245; interleukin 17 receptor B; interleukin 17 receptor homolog 1; interleukin-17 receptor B; Interleukin-17B receptor

Product Datasheets

Citations for Mouse IL-17RB Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. IL-25 improves diabetic wound healing through stimulating M2 macrophage polarization and fibroblast activation
    Authors: S Li, X Ding, H Zhang, Y Ding, Q Tan
    International immunopharmacology, 2022;106(0):108605.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  2. IL-25 exacerbates autoimmune aortitis in IL-1 receptor antagonist-deficient mice
    Authors: T Yoshizaki, S Itoh, S Yamaguchi, T Numata, A Nambu, N Kimura, H Suto, K Okumura, K Sudo, A Yamaguchi, S Nakae
    Sci Rep, 2019;9(1):17067.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. IL-25 enhances Th17 cell-mediated contact dermatitis by promoting IL-1? production by dermal dendritic cells
    Authors: H Suto, A Nambu, H Morita, S Yamaguchi, T Numata, T Yoshizaki, E Shimura, K Arae, Y Asada, K Motomura, M Kaneko, T Abe, A Matsuda, Y Iwakura, K Okumura, H Saito, K Matsumoto, K Sudo, S Nakae
    J. Allergy Clin. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. RORgammat+ innate lymphoid cells regulate intestinal homeostasis by integrating negative signals from the symbiotic microbiota.
    Authors: Sawa S, Lochner M, Satoh-Takayama N, Dulauroy S, Berard M, Kleinschek M, Cua D, Di Santo JP, Eberl G
    Nat. Immunol., 2011;12(4):320-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
    Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
    J. Immunol., 2011;188(3):1503-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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