Mouse IL-1 beta /IL-1F2 Antibody

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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody.
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Mouse IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Detects mouse IL-1 beta /IL-1 F2 in direct ELISAs and Western blots. In Western blots, shows 100% cross-reactivity with recombinant rat (rr) IL‑1 beta and rcrIL‑1 beta, 15%‑35 % with rhIL‑1 beta and rpIL‑1 beta, and no cross-reactivity with rmIL-1 alpha, rmIL-18, or rhIL-1 beta rp.
Monoclonal Hamster IgG Clone # B122
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse IL-1 beta
Accession # P10749
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML)
Hogquist, K.A. et al. (1991) J. Immunol. 146:1534.
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4012). The ND50 is typically 0.3-1.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Citations for Mouse IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death
    Authors: T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek
    Cell. Signal., 2020-06-15;73(0):109690.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Neutralization, Western Blot
  2. Mycobacterium tuberculosis Limits Host Glycolysis and IL-1&beta by Restriction of PFK-M via MicroRNA-21
    Authors: EE Hackett, H Charles-Me, SM O'Leary, LE Gleeson, N Muñoz-Wolf, S Case, A Wedderburn, DGW Johnston, MA Williams, A Smyth, M Ouimet, KJ Moore, EC Lavelle, SC Corr, SV Gordon, J Keane, FJ Sheedy
    Cell Rep, 2020-01-07;30(1):124-136.e4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Nicotinamide phosphoribosyltransferase aggravates inflammation and promotes atherosclerosis in ApoE knockout mice
    Authors: YY Kong, GQ Li, WJ Zhang, X Hua, CC Zhou, TY Xu, ZY Li, P Wang, CY Miao
    Acta Pharmacol. Sin., 2019-03-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Cholinergic anti-inflammatory pathway inhibits neointimal hyperplasia by suppressing inflammation and oxidative stress
    Authors: DJ Li, H Fu, J Tong, YH Li, LF Qu, P Wang, FM Shen
    Redox Biol, 2017-11-16;15(0):22-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Key Role of the Scavenger Receptor MARCO in Mediating Adenovirus Infection and Subsequent Innate Responses of Macrophages
    Authors: MD Maler, PJ Nielsen, N Stichling, I Cohen, Z Ruzsics, C Wood, P Engelhard, M Suomalaine, I Gyory, M Huber, J Müller-Que, WWA Schamel, S Gordon, T Jakob, SF Martin, W Jahnen-Dec, UF Greber, MA Freudenber, G Fejer
    MBio, 2017-08-01;8(4):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1alpha-dependent in mice.
    Authors: Botelho F, Nikota J, Bauer C, Morissette M, Iwakura Y, Kolbeck R, Finch D, Humbles A, Stampfli M
    Respir Res, 2012-09-19;13(0):81.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
    Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
    J. Immunol., 2011-09-19;187(9):4835-43.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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Mouse IL-1 beta /IL-1F2 Antibody
By Anonymous on 11/21/2017
Application: ELISA Sample Tested: Serum and Plasma,Tissue Culture Media Species: Mouse

This antibody was paired with BAF401 to create an ELISA to measure IL-1beta in mouse samples.