Mouse IL-2 Quantikine ELISA Kit

(34 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL)
  • Sensitivity
    3 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant mouse IL-2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-2 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-2 accurately. Results obtained using natural mouse IL-2 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean70.418759570.9175559
Standard Deviation3.91023.43.69.624.5
CV%5.55.33.95.15.54.4

Recovery

The recovery of mouse IL-2 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 102 84-110
Serum (n=6) 102 96-108
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-2 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse IL-2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-2
IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.
    • Long Name
      Interleukin 2
    • Entrez Gene IDs
      3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit);
    • Alternate Names
      aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    34 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Viral RNA-Unprimed Rig-I Restrains Stat3 Activation in the Modulation of Regulatory T Cell/Th17 Cell Balance
      Authors: H Yang, HZ Guo, XY Li, J Lin, W Zhang, JM Zhao, HX Zhang, SJ Chen, Z Chen, J Zhu
      J. Immunol., 2017;0(0):.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    2. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology
      Authors: SJ Whitfield, C Taylor, JE Risdall, GD Griffiths, JT Jones, ED Williamson, S Rijpkema, L Saraiva, S Vessillier, AC Green, AJ Carter
      J. Immunol, 2017;0(0):.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    3. Death receptor 6 contributes to autoimmunity in lupus-prone mice
      Authors: D Fujikura, M Ikesue, T Endo, S Chiba, H Higashi, T Uede
      Nat Commun, 2017;8(0):13957.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    4. Use of Recombinant Virus Replicon Particles for Vaccination against Mycobacterium ulcerans Disease.
      Authors: Bolz M, Kerber S, Zimmer G, Pluschke G
      PLoS Negl Trop Dis, 2015;9(8):e0004011.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    5. IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance.
      Authors: Basu R, Whitley S, Bhaumik S, Zindl C, Schoeb T, Benveniste E, Pear W, Hatton R, Weaver C
      Nat Immunol, 2015;16(3):286-95.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. MDSC and TGFbeta Are Required for Facilitation of Tumor Growth in the Lungs of Mice Exposed to Carbon Nanotubes.
      Authors: Shvedova A, Kisin E, Yanamala N, Tkach A, Gutkin D, Star A, Shurin G, Kagan V, Shurin M
      Cancer Res, 2015;75(8):1615-23.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    7. Diisopropylamine dichloroacetate, a novel pyruvate dehydrogenase kinase 4 inhibitor, as a potential therapeutic agent for metabolic disorders and multiorgan failure in severe influenza.
      Authors: Yamane, Kazuhiko, Indalao, Irene L, Chida, Junji, Yamamoto, Yoshikaz, Hanawa, Masaaki, Kido, Hiroshi
      PLoS ONE, 2014;9(5):e98032.
      Species: Mouse
      Sample Type: Tissue Homogenates
    8. Celecoxib sensitizes Staphylococcus aureus to antibiotics in macrophages by modulating SIRT1.
      Authors: Annamanedi M, Kalle A
      PLoS ONE, 2014;9(6):e99285.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Suppression of the macrophage proteasome by ethanol impairs MHC class I antigen processing and presentation.
      Authors: D'Souza, Alain J, Desai, Shyamal, Rudner, Xiaowen, Kelly, Michelle, Ruan, SanBao, Shellito, Judd E
      PLoS ONE, 2013;8(2):e56890.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    10. Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
      Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
      J. Biol. Chem., 2012;287(20):16356-64.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    11. The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
      Authors: Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S
      J. Immunol., 2012;189(9):4237-46.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    12. Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
      Authors: Sun J, Fu F, Gu W, Yan R, Zhang G, Shen Z, Zhou Y, Wang H, Shen B, Zhang X
      PLoS ONE, 2011;6(9):e24751.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    13. Control of mouse graft-versus-host disease following allogeneic bone marrow transplantation by blocking the CD28/B7 signaling pathway with lentiviral vector-mediated RNA interference.
      Authors: Sang W, Zhou C, Cheng N, Li Z, Zeng L, Xu K
      Immunol. Lett., 2011;136(2):194-202.
      Species: Mouse
      Sample Type: Plasma
    14. Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
      Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
      Eur. J. Immunol., 2009;39(1):85-95.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    15. The adjuvancy of OX40 ligand (CD252) on an HIV-1 canarypox vaccine.
      Authors: Liu J, Ngai N, Stone GW, Yue FY, Ostrowski MA
      Vaccine, 2009;27(37):5077-84.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    16. Dietary curcumin and limonin suppress CD4+ T-cell proliferation and interleukin-2 production in mice.
      Authors: Kim W, Fan YY, Smith R, Patil B, Jayaprakasha GK, McMurray DN, Chapkin RS
      J. Nutr., 2009;139(5):1042-8.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    17. The LTK63 adjuvant improves protection conferred by Ag85B DNA-protein prime-boosting vaccination against Mycobacterium tuberculosis infection by dampening IFN-gamma response.
      Authors: Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Fattorini L, Del Giudice G, Cassone A
      Vaccine, 2008;26(33):4237-43.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    18. Dendritic cells derived from TBP-2-deficient mice are defective in inducing T cell responses.
      Authors: Son A, Nakamura H, Okuyama H, Oka S, Yoshihara E, Liu W, Matsuo Y, Kondo N, Masutani H, Ishii Y, Iyoda T, Inaba K, Yodoi J
      Eur. J. Immunol., 2008;38(5):1358-67.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    19. Thymic stromal lymphopoietin and thymic stromal lymphopoietin-conditioned dendritic cells induce regulatory T-cell differentiation and protection of NOD mice against diabetes.
      Authors: Besin G, Gaudreau S, Menard M, Guindi C, Dupuis G, Amrani A
      Diabetes, 2008;57(8):2107-17.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    20. Lipopolysaccharide-activated IL-10-secreting dendritic cells suppress experimental autoimmune uveoretinitis by MHCII-dependent activation of CD62L-expressing regulatory T cells.
      Authors: Lau AW, Biester S, Cornall RJ, Forrester JV
      J. Immunol., 2008;180(6):3889-99.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    21. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity.
      Authors: Yamanouchi J, Rainbow D, Serra P, Howlett S, Hunter K, Garner VE, Gonzalez-Munoz A, Clark J, Veijola R, Cubbon R, Chen SL, Rosa R, Cumiskey AM, Serreze DV, Gregory S, Rogers J, Lyons PA, Healy B, Smink LJ, Todd JA, Peterson LB, Wicker LS, Santamaria P
      Nat. Genet., 2007;39(3):329-37.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    22. Intercellular adhesion molecule-1 expression is required on multiple cell types for the development of experimental autoimmune encephalomyelitis.
      Authors: Bullard DC, Hu X, Schoeb TR, Collins RG, Beaudet AL, Barnum SR
      J. Immunol., 2007;178(2):851-7.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    23. The endothelial cell-produced antiangiogenic cytokine vascular endothelial growth inhibitor induces dendritic cell maturation.
      Authors: Tian F, Grimaldo S, Fujita M, Fugita M, Cutts J, Vujanovic NL, Li LY
      J. Immunol., 2007;179(6):3742-51.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    24. Granulocyte-macrophage colony-stimulating factor prevents diabetes development in NOD mice by inducing tolerogenic dendritic cells that sustain the suppressive function of CD4+CD25+ regulatory T cells.
      Authors: Gaudreau S, Guindi C, Menard M, Besin G, Dupuis G, Amrani A
      J. Immunol., 2007;179(6):3638-47.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    25. Suppression of established experimental autoimmune uveitis by anti-LFA-1alpha Ab.
      Authors: Ke Y, Sun D, Zhang P, Jiang G, Kaplan HJ, Shao H
      Invest. Ophthalmol. Vis. Sci., 2007;48(6):2667-75.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    26. Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation.
      Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X
      Biochem. Biophys. Res. Commun., 2006;345(3):1122-30.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    27. Tetrathiomolybdate is effective in a mouse model of arthritis.
      Authors: McCubbin MD, Hou G, Abrams GD, Dick R, Brewer GJ
      J. Rheumatol., 2006;33(12):2501-6.
      Species: Mouse
      Sample Type: Serum
    28. Blockade of transforming growth factor-{beta} signaling in tumor-reactive CD8(+) T cells activates the antitumor immune response cycle.
      Authors: Zhang Q, Yang XJ, Kundu SD, Pins M, Javonovic B, Meyer R, Kim SJ, Greenberg NM, Kuzel T, Meagher R, Guo Y, Lee C
      Mol. Cancer Ther., 2006;5(7):1733-43.
      Species: Mouse
      Sample Type: Serum
    29. PKCtheta and PKA are antagonistic partners in the NF-AT transactivation pathway of primary mouse CD3+ T lymphocytes.
      Authors: Hermann-Kleiter N, Thuille N, Pfeifhofer C, Gruber T, Schafer M, Zitt C, Hatzelmann A, Schudt C, Leitges M, Baier G
      Blood, 2006;107(12):4841-8.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    30. Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
      Authors: Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M
      J. Neurosci., 2004;24(27):6133-43.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    31. EphrinB1 is essential in T-cell-T-cell co-operation during T-cell activation.
      Authors: Yu G, Luo H, Wu Y, Wu J
      J. Biol. Chem., 2004;279(53):55531-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    32. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
      Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
      Comp. Immunol. Microbiol. Infect. Dis., 2004;27(6):393-411.
      Species: Mouse
      Sample Type: Serum
    33. Anti-tumor mechanism of Z-100, an immunomodulatory Arabinomannan extracted from Mycobacterium tuberculosis strain Aoyama B, on pulmonary metastases of B16F10 melanoma: restoration of helper T cell responses via suppression of glucocorticoid-genesis.
      Authors: Oka H, Emori Y, Sasaki H, Shiraishi Y, Yoshinaga K, Kurimoto T
      Microbiol. Immunol., 2002;46(5):343-51.
    34. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
      Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
      Eur. J. Immunol., 2001;31(7):2104-15.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    Expand to show all 34 Citations
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