Mouse IL-2 Quantikine ELISA Kit

R&D Systems | Catalog # M2000

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL)

Sensitivity

3 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Serum)
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Product Summary for Mouse IL-2 Quantikine ELISA Kit

The Quantikine Mouse IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-2 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-2 accurately. Results obtained using natural mouse IL-2 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-2.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse IL-2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 70.4 187 595 70.9 175 559
Standard Deviation 3.9 10.0 23.4 3.6 9.6 24.5
CV% 5.5 5.3 3.9 5.1 5.5 4.4

Recovery for Mouse IL-2 Quantikine ELISA Kit

The recovery of mouse IL-2 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 102 84-110
Serum (n=6) 102 96-108

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-2 in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse IL-2 ELISA Linearity

Scientific Data Images for Mouse IL-2 Quantikine ELISA Kit

Mouse IL-2 ELISA Standard Curve

Mouse IL-2 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

IL-2 (Interleukin-2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and  memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

Additional IL-2 Products

Product Documents for Mouse IL-2 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-2 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse IL-2 Quantikine ELISA Kit

Customer Reviews for Mouse IL-2 Quantikine ELISA Kit (7)

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  • Mouse IL-2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 12/12/2022
    Virus-infected Cell culture supernatant diluted 1:10,000
    Mouse IL-2 Quantikine ELISA Kit M2000
  • Mouse IL-2 Quantikine ELISA Kit
    Name: Thanh Nguyen
    Sample Tested: activated mouse CD8 T cell
    Verified Customer | Posted 10/18/2020
  • Mouse IL-2 Quantikine ELISA Kit
    Name: Dhivya Sridaran
    Sample Tested: MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
    Verified Customer | Posted 04/04/2019
  • Mouse IL-2 Quantikine ELISA Kit
    Name: Shambhavi Bhagwat
    Sample Tested: activated mouse CD8 T cell
    Verified Customer | Posted 03/21/2019
  • Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 06/19/2018
  • Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 06/12/2018
  • Name: Hannah White
    Sample Tested: Concanavalin A-stimulated splenocytes
    Verified Customer | Posted 05/24/2017

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Protocols

View specific protocols for Mouse IL-2 Quantikine ELISA Kit (M2000):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Mouse IL-2 Quantikine ELISA Kit

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  • Q: Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?

    A: A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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