Mouse IL-20 R beta Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-20 R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human IL‑20 R beta is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL‑20 R beta
    Asp14-Asn215
    Accession # NP_001032323
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑20 R beta Fc Chimera (Catalog # 4388-MR)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑20 R beta in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse IL‑20 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4388, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-20 R beta

IL-20 receptor beta (IL-20 R beta ), also known as IL-20 R2, is a type I transmembrane glycoprotein in the class II cytokine receptor family. These receptors are characterized by tandem fibronectin type III domains in their extracellular region and the lack of a WSXWS motif (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). Based on the structure of the human receptor, mouse IL-20 R beta should contain a 202 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains, a 16 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, mouse IL-20 R beta will share 81% aa sequence identity with human IL-20 R beta. It shares 18-29% aa sequence identity with the mouse class II cytokine receptors IFN-alpha R1, IFN-alpha R2, IFN-gamma R1, IFN-gamma R2, IL-10 R alpha, IL-10 R beta, IL-20 R alpha, IL-22BP, IL-22 R alpha, IL-28 R, and tissue factor. IL-20 R beta is generally expressed in psoriatic skin (keratinocytes and select endothelium), rheumatoid arthritis synovial membranes, and hepatocytes of LPS-treated mice, and it contributes to the local inflammatory reaction (3-6). IL-20 R beta heterodimerizes with IL-20 R alpha to form the receptor complex that mediates IL-19, IL-20, and IL-24 signals (3, 7-10). It also heterodimerizes with IL-22 R to form the functional receptor complex for IL-20 and IL-24 (7-9). Binding of these IL-10 family class II cytokines to their receptors induces activation of the JAK-STAT signal transduction pathway.

 

  • References:
    1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 
    2. Accession # NP_001032323 and SwissProt # Q6UXL0.
    3. Blumberg, H. et al. (2001) Cell 104:9. 
    4. Hsu, Y-H. et al. (2006) Arthritis Rheum. 54:2722. 
    5. Wegenka, U.M. et al. (2007) J. Hepatol. 46:257. 
    6. Chan, J.R. et al. (2006) J. Exp. Med. 203:2577.
    7. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
    8. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
    9. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
    10. Pletnev, S. et al. (2003) Biochemistry 42:12617.
  • Long Name:
    Interleukin 20 Receptor beta
  • Entrez Gene IDs:
    53833 (Human); 213208 (Mouse)
  • Alternate Names:
    FNDC6; DIRS1; FNDC6; IL-20 R beta; IL20R beta; IL-20R2; IL20RB interleukin 20 receptor beta; IL20RB; IL-20Rb; MGC34923
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy
    Authors: YH Hsu, HH Li, JM Sung, WY Chen, YC Hou, YH Weng, WT Lai, CH Wu, MS Chang
    Exp. Mol. Med., 2017;49(3):e310.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fresh
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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