Recombinant Mouse IL-21 Protein (Catalog # 594-ML)
Measured by its ability to neutralize IL‑21-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 250 ng/mL Recombinant Mouse IL‑21.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑21 and Neutralization by Mouse IL‑21 Antibody. Recombinant Mouse IL‑21 (Catalog # 594-ML) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑21 (250 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594). The ND50 is typically 5-15 µg/mL.
IL‑21 in Mouse Splenocytes. IL‑21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid sequence identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL‑21/IL‑21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.
Parrish-Novak, et al. (2000) Nature 408:57.
Ozaki, K. et al. (2000) PNAS 97:11439.
Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
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