|Cell Proliferation Induced by IL‑21 and Neutralization by Mouse IL‑21 Antibody. Recombinant Mouse IL‑21 (Catalog # 594-ML) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑21 (250 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594). The ND50 is typically 5-15 µg/mL.|
|IL‑21 in Mouse Splenocytes. IL‑21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid sequence identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL‑21/IL‑21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12 Citations: Showing 1 - 10
Filter your results:
Expand to show all 12 Citations