Mouse IL-21 Antibody AF594: R&D Systems

Mouse IL-21 Antibody

(12 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-21 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL-15, and recombinant human IL-21 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
    Pro25-Ser146
    Accession # Q9ES17
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.30 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑21 (Catalog # 594-ML)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Mouse IL-21 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse IL‑21 Antibody (Catalog # AF594)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse IL‑21 Biotinylated Antibody (Catalog # BAF594)
  • ELISA Standard
     
    Recombinant Mouse IL-21 Protein (Catalog # 594-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑21-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 250 ng/mL Recombinant Mouse IL‑21.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑21 and Neutralization by Mouse IL‑21 Antibody. Recombinant Mouse IL‑21 (Catalog # 594-ML) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑21 (250 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594). The ND50 is typically 5-15 µg/mL.
Immunocytochemistry
IL‑21 in Mouse Splenocytes. IL‑21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-21

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid sequence identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL‑21/IL‑21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

  • References:
    1. Parrish-Novak, et al. (2000) Nature 408:57.
    2. Ozaki, K. et al. (2000) PNAS 97:11439.
    3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
    4. Asao, H. et al. (2001) J. Immunol. 167:1.
  • Long Name:
    Interleukin 21
  • Entrez Gene IDs:
    59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)
  • Alternate Names:
    IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Intestinal microbiota link lymphopenia to murine autoimmunity via PD-1(+)CXCR5(-/dim) B-helper T cell induction
    Authors: T Eri, K Kawahata, T Kanzaki, M Imamura, K Michishita, L Akahira, E Bannai, N Yoshikawa, Y Kimura, T Satoh, S Uematsu, H Tanaka, K Yamamoto
    Sci Rep, 2017;7(0):46037.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. Prostaglandin E2 inhibits Tr1 cell differentiation through suppression of c-Maf
    Authors: KM Hooper, W Kong, D Ganea
    PLoS ONE, 2017;12(6):e0179184.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  3. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015;290(37):22338-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Interleukin-21 is critically required in autoimmune and allogeneic responses to islet tissue in murine models.
    Authors: McGuire HM, Walters S, Vogelzang A
    Diabetes, 2011;60(3):867-75.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. STAT3-dependent IL-21 production from T helper cells regulates hematopoietic progenitor cell homeostasis.
    Authors: Kaplan MH, Glosson NL, Stritesky GL, Yeh N, Kinzfogl J, Rohrabaugh SL, Goswami R, Pham D, Levy DE, Brutkiewicz RR, Blum JS, Cooper S, Hangoc G, Broxmeyer HE
    Blood, 2011;117(23):6198-201.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).
    Authors: Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW
    PLoS ONE, 2010;5(1):e8720.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  7. Cytokine-secreting follicular T cells shape the antibody repertoire.
    Authors: Reinhardt RL, Liang HE, Locksley RM
    Nat. Immunol., 2009;10(4):385-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ELISpot Development
  8. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008;112(2):362-73.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  9. IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner.
    Authors: Wei L, Laurence A, Elias KM, O'Shea JJ
    J. Biol. Chem., 2007;282(48):34605-10.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  10. Expression and one-step purification of bovine interleukin-21 (IL-21) in silkworms using a hybrid baculovirus expression system.
    Authors: Muneta Y, Nagaya H, Minagawa Y, Enomoto C, Matsumoto S, Mori Y
    Biotechnol. Lett., 2004;26(18):1453-8.
    Species: Bovine
    Sample Type: Recombinant Protein
    Application: WB
  11. IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
    Authors: Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S
    J. Immunol., 2004;172(3):1540-7.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. IL-21 induces the functional maturation of murine NK cells.
    Authors: Brady J, Hayakawa Y, Smyth MJ, Nutt SL
    J. Immunol., 2004;172(4):2048-58.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Expand to show all 12 Citations
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