Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Bovine, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Capture, ELISA Development, ELISpot Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-21 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
Pro25-Ser146
Accession # Q9ES17

Specificity

Detects mouse IL-21 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL-15, and recombinant human IL-21 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL-21 Antibody

IL‑21 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

IL‑21 in Mouse Splenocytes.

IL‑21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL-21 and Neutralization by Mouse IL-21 Antibody.

Recombinant Mouse IL-21 (Catalog # 594-ML) stimulates proliferation in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594). The ND50 is typically 0.2-2 µg/mL.
Mouse IL-21 ELISA Standard Curve

Mouse IL-21 ELISA Standard Curve

Recombinant Mouse IL‑21 (Catalog # 594-ML) was serially diluted and captured by Goat Anti-Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Mouse IL-21 ELISA Standard Curve

Mouse IL-21 ELISA Standard Curve

Recombinant Mouse IL‑21 (Catalog # 594-ML) was serially diluted and captured by Goat Anti-Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse IL-21 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed concanavalin A-stimulated mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑21 (Catalog # 594-ML)

Neutralization

Measured by its ability to neutralize IL‑21-induced proliferation in the NK-92 human natural killer lymphoma cell line. The Neutralization Dose (ND50) is typically 0.2-2 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑21.

Mouse IL-21 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse IL‑21 Biotinylated Antibody (Catalog # BAF594)
  • Standard: Recombinant Mouse IL-21 Protein (Catalog # 594-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF594 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-21

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid sequence identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL‑21/IL‑21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

References

  1. Parrish-Novak, et al. (2000) Nature 408:57.
  2. Ozaki, K. et al. (2000) PNAS 97:11439.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Asao, H. et al. (2001) J. Immunol. 167:1.

Long Name

Interleukin 21

Alternate Names

CVID11, IL21, Za11

Entrez Gene IDs

59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)

Gene Symbol

IL21

UniProt

Q9ES17

Additional IL-21 Products

Product Documents for Mouse IL-21 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-21 Antibody

For research use only

Citations for Mouse IL-21 Antibody

Customer Reviews for Mouse IL-21 Antibody (1)

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  • Mouse IL-21 Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 11/14/2022
    Worked well as a capture spot for our Mouse Elisa.
    Mouse IL-21 Antibody AF594

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