Mouse IL-24 Antibody (303308)

IL‑24 in Mouse Splenocytes.

IL-24 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse IL-24 Monoclonal Antibody (Catalog # MAB2786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of IL-24 by Flow Cytometry

Characterization of depletion of CD4+ CD25+ Foxp3+ T cells in immunized mice. A–E Representative dot plots data demonstrating the frequency of CD4+ CD25+ Foxp3+ lymphocytes in the splenocytes of various immunized mice groups. Splenocytes were collected one week after last intratumoral injection of DNA vaccine and adjuvant, then subjected to flow cytometry analysis using the anti-CD4, anti-CD25, and anti-Foxp + antibodies. F Bar figures represent the percentages of CD4+ CD25+ Foxp3+ T cells (Treg) among CD4+ T cells from splenocytes. Mice treated with DNA E7 plus IL-24 had the lowest numbers of Treg cells from splenocytes among the other groups (*** p < 0.001). Data demonstrate mean ± one standard error of two independent experiments Image collected and cropped by CiteAb from the following open publication (https://virologyj.biomedcentral.com/articles/10.1186/s12985-022-01842-x), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-24 by Immunohistochemistry

Deletion of the IL-24 receptor in tumor cells blocks the antitumor effect of calcipotriol on mammary tumors.(A) Representative IF images of IL-24, IL-22R alpha, and cleaved caspase-3–stained PyMt mammary tumor in calcipotriol-treated WT mice. Dotted circles outline a tumor focus. (B) Schematic diagram of the experimental setup to assess the contribution of the IL-24/IL-20R axis to the efficacy of topical calcipotriol treatment against PyMt mammary tumor growth. (C) Representative macroscopic images of WT and Il20rb–/– PyMt mammary tumors in WT mice (dotted circles highlight the tumor sites). (D and E) WT versus Il20rb–/– PyMt mammary tumor volume over time in calcipotriol-treated WT mice presented as (D) mean tumor volumes + SD and (E) spider plot (n = 10 in each group). Two-way ANOVA (D and E). Scale bars: 100 μm (A) and 1 cm (C). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39782693), licensed under a CC-BY license. Not internally tested by R&D Systems.