Mouse IL-24 Antibody (303308)

R&D Systems | Catalog # MAB2786

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunofluorescence, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 303308
View all IL-24 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-24
Arg27-His181
Accession # NP_444325

Specificity

Detects mouse IL-24 in direct ELISAs and Western blots. No cross-reactivity with recombinant human IL-24 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse IL-24 Antibody (303308)

IL-24 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

IL‑24 in Mouse Splenocytes.

IL-24 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse IL-24 Monoclonal Antibody (Catalog # MAB2786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse IL-24 Antibody (303308)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL-24

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-24

Mouse IL-24, also known as melanoma differentiation-associated gene 7 (MDA-7) and Th2-specific cytokine FISP, is a 27 kDa secreted glycoprotein that is a member of the IL-10 family. It is synthesized as a precursor with a large pro-region that is removed to generate a 155 aa mature protein. Mature mouse IL-24 shares 84% and 69% amino acid identity with rat and human IL-24, respectively.

Long Name

Interleukin 24

Alternate Names

C49A, FISP, IL24, MDA-7, MOB-5, ST16

Entrez Gene IDs

11009 (Human); 93672 (Mouse); 170819 (Rat)

Gene Symbol

IL24

UniProt

NP_444325

Additional IL-24 Products

Product Documents for Mouse IL-24 Antibody (303308)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-24 Antibody (303308)

For research use only

Citations for Mouse IL-24 Antibody (303308)

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Protocols

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