IL‑24 in Mouse Splenocytes.
IL‑24 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse IL‑24 Monoclonal Antibody (Catalog # MAB2786) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse IL-24, also known as melanoma differentiation-associated gene 7
(MDA-7) and Th2-specific cytokine FISP, is a 27 kDa secreted
glycoprotein that is a member of the IL-10 family. It is synthesized as
a precursor with a large pro-region that is removed to generate a
155 aa mature protein. Mature mouse IL-24 shares 84% and 69% amino acid
identity with rat and human IL-24, respectively.
Entrez Gene IDs:
11009 (Human); 93672 (Mouse); 170819 (Rat)
C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein
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