Mouse IL-34 Antibody

Catalog # Availability Size / Price Qty
AF5195
AF5195-SP
Detection of Mouse IL‑34 by Western Blot.
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Product Details
Citations (8)
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Mouse IL-34 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-34 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL-34 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑34
Asn21-Pro235
Accession # Q8R1R4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑34-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. The Neutralization Dose (ND50) is typically 0.3-1.5 μg/ml in the presence of 100 ng/ml Recombinant Mouse IL‑34.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IL‑34 antibody by Western Blot. View Larger

Detection of Mouse IL‑34 by Western Blot. Western blot shows lysates of mouse spleen tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse IL-34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5195) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-34 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Neutralization Cell Proliferation Induced by IL‑34 and Neutralization by Mouse IL‑34 Antibody. View Larger

Cell Proliferation Induced by IL‑34 and Neutralization by Mouse IL‑34 Antibody. Recombinant Mouse IL-34 (Catalog # 5195-ML) induces proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-34 (100 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse IL-34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5195). The ND50 is typically 0.3-1.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77 homolog) is a 39 kDa, secreted cytokine that belongs to no known cytokine family (1). Mouse IL-34 is synthesized as a 235 amino acid (aa) precursor with a 20 aa signal sequence and a 215 aa mature chain (SwissProt # Q8R1R4). The mature chain contains two potential sites for N-linked glycosylation. There are three isoforms for IL-34. Isoform 1 (Q8R1R4-1) is the recombinant mouse IL-34 described in this insert. Isoform 2 (Q8R1R4-2) has an 85 aa substitution for the final 101 aa’s in isoform 1, and isoform 3 (Q8R1R4-3) lacks Q81 in isoform 1. Mouse IL-34 is 71% identical to human IL-34 on the amino acid level. IL-34 is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and it is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF‑1R) (1). IL-34 stimulates monocyte viability (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

References
  1. Lin, H. et al. (2008) Science 320:807.
Long Name
Interleukin 34
Entrez Gene IDs
146433 (Human); 76527 (Mouse)
Alternate Names
C16orf77; IL34; IL-34; interleukin 34

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Citations for Mouse IL-34 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Nrf2 deficiency causes hepatocyte dedifferentiation and reduced albumin production in an experimental extrahepatic cholestasis model
    Authors: GY Wang, V Garcia, J Lee, J Yanum, J Lin, H Jiang, G Dai
    PLoS ONE, 2022-06-13;17(6):e0269383.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  2. Reno-protective effect of IL-34 inhibition on cisplatin-induced nephrotoxicity in mice
    Authors: Y Wada, M Iyoda, K Matsumoto, T Suzuki, S Tachibana, N Kanazawa, H Honda
    PLoS ONE, 2021-01-11;16(1):e0245340.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  3. Conditional genetic deletion of CSF1 receptor in microglia ameliorates the physiopathology of Alzheimer's disease.
    Authors: Pons V, Levesque P, Plante M, Rivest S
    Alzheimers Res Ther, 2021-01-05;13(1):8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Inhibition of IL-34 Unveils Tissue-Selectivity and Is Sufficient to Reduce Microglial Proliferation in a Model of Chronic Neurodegeneration.
    Authors: Obst J, Simon E, Martin-Estebane M, Pipi E, Barkwill L, Gonzalez-Rivera I, Buchanan F, Prescott A, Faust D, Fox S, Brownlees J, Taylor D, Perry V, Nuthall H, Atkinson P, Karran E, Routledge C, Gomez-Nicola D
    Front Immunol, 2020-10-08;11(0):579000.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  5. IL-34 cell surface localization regulated by the 78 kDa glucose-regulated protein facilitates the differentiation of monocytic cells
    Authors: S Ogawa, Y Matsuoka, M Takada, K Matsui, F Yamane, E Kubota, S Yasuhara, K Hieda, N Kanayama, N Hatano, H Tokumitsu, M Magari
    J. Biol. Chem., 2018-12-20;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  6. IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease.
    Authors: Baek J, Zeng R, Weinmann-Menke J, Valerius M, Wada Y, Ajay A, Colonna M, Kelley V
    J Clin Invest, 2015-06-29;125(8):3198-214.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Bioassay, Neutralization
  7. CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.
    Authors: Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley E, Kanayama N, Mabbott N, Magari M, Ohmori H
    J Leukoc Biol, 2013-09-19;95(1):19-31.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Interleukin-34 selectively enhances the neuroprotective effects of microglia to attenuate oligomeric amyloid-beta neurotoxicity.
    Authors: Mizuno T, Doi Y, Mizoguchi H, Jin S, Noda M, Sonobe Y, Takeuchi H, Suzumura A
    Am. J. Pathol., 2011-08-26;179(4):2016-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot

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