Mouse IL-36Ra/IL-1F5 Antibody

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Detection of Mouse IL‑36Ra/IL‑1F5 by Western Blot.
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Mouse IL-36Ra/IL-1F5 Antibody Summary

Species Reactivity
Detects mouse IL-36Ra/IL-1F5 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human IL-36Ra and recombinant mouse IL-1ra is observed.
Monoclonal Rat IgG2B Clone # 759207
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse IL-36Ra/IL-1F5
Accession # Q9QYY1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IL-36Ra/IL-1F5 antibody by Western Blot. View Larger

Detection of Mouse IL‑36Ra/IL‑1F5 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-36Ra/IL-1F5 Monoclonal Antibody (Catalog # MAB2714) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-36Ra/IL-1F5 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36Ra/IL-1F5

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1δ [delta], IL‑1HY1, IL-1H3, and IL-1L1) is a member of the IL‑1 family of proteins (1-6). IL‑1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL‑18 and IL‑1F5 through F10 (6, 7). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications (8). The mouse IL‑36Ra/IL‑1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL‑18), supporting an evolutionary relationship with the IL-1 family (1, 9). It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. IL‑36Ra/IL‑1F5 is synthesized as a 156 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5, 8). Nevertheless, it appears to be secreted as a 17 kDa monomer. In humans, there is an alternate start site that potentially gives rise to an alternate splice form (5). This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL‑36Ra/IL‑1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL‑1ra, IL‑1 beta, IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL-36 beta /IL-1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). Indirect evidence suggests the receptor for IL-36Ra/IL-1F5 is IL‑1 Rrp2 and/or IL-1 RAcP (9). IL-36Ra/IL-1F5 was initially reported to be an antagonist of  IL-36 gamma activity (4, 7). This would be consistent with its hypothesized relationship to IL‑1ra.

  1. Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
  4. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  5. Barton J. L. et al. (2000) Eur. J. Immunol. 30:3299.
  6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  7. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  8. Debets, R. et al. (2001) J. Immunol. 167:1440.
  9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5
Entrez Gene IDs
26525 (Human); 54450 (Mouse)
Alternate Names
FIL1 delta; FIL1; FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta); FIL1DIL-1 delta; IL1F5 (Canonical product IL-1F5a); IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; interleukin 1 family, member 5 (delta); interleukin 1, delta; Interleukin 36 Receptor Antagonist; interleukin-1 family member 5; Interleukin-1 HY1; Interleukin-1 receptor antagonist homolog 1; Interleukin-1-like protein 1; Interleukin-36 Receptor Antagonist

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