Mouse Integrin alpha 2/CD49b Biotinylated Antibody Summary
Accession # Q62469
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Integrin alpha 2/CD49b in Mouse Splenocytes. Integrin alpha 2/CD49b was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Integrin alpha 2/CD49b Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 2/CD49b
The Integrin family proteins are heterodimeric transmembrane receptors comprised of an alpha and a beta subunit. The Integrin alpha 2 subunit is expressed in monocytes, platelets, B, T and NK cells. It forms a non-covalent heterodimer with the Integrin beta 1 subunit. Integrin alpha 2/ beta 1 binds collagen and laminin and activates intracellular signaling pathways.
Citation for Mouse Integrin alpha 2/CD49b Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Indoleamine 2,3-dioxygenase promotes peritoneal dissemination of ovarian cancer through inhibition of natural killercell function and angiogenesis promotion.
Authors: Nonaka H, Saga Y, Fujiwara H
Int. J. Oncol., 2011;38(1):113-20.
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded
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