Detects mouse Integrin alpha 2/CD49b in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Integrin alpha M is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 4, rmIntegrin alpha 5, and rmIntegrin alpha E is observed.
Integrin alpha 2/CD49b in Mouse Splenocytes.
Integrin alpha 2/CD49b was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Integrin alpha 2/CD49b Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 2/CD49b
The Integrin family proteins are heterodimeric transmembrane receptors comprised of an alpha and a beta subunit. The Integrin alpha 2 subunit is expressed in monocytes, platelets, B, T and NK cells. It forms a non-covalent heterodimer with the Integrin beta 1 subunit. Integrin alpha 2/ beta 1 binds collagen and laminin and activates intracellular signaling pathways.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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