Mouse Integrin  alpha 2/CD49b Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1740
Integrin  alpha 2/CD49b  in Mouse Splenocytes.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse Integrin  alpha 2/CD49b Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 2/CD49b in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Integrin  alpha M is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 4, rmIntegrin alpha 5, and rmIntegrin alpha E is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b
Tyr27-Thr1129
Accession # Q62469
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Integrin  alpha 2/CD49b
Flow Cytometry
0.25 µg/106 cells
Mouse DX5+ splenocytes
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Integrin  alpha 2/CD49b  in Mouse Splenocytes. View Larger

Integrin  alpha 2/CD49b in Mouse Splenocytes. Integrin  alpha 2/CD49b was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Integrin  alpha 2/CD49b Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2/CD49b

The Integrin family proteins are heterodimeric transmembrane receptors comprised of an alpha and a beta subunit. The Integrin alpha 2 subunit is expressed in monocytes, platelets, B, T and NK cells. It forms a non-covalent heterodimer with the Integrin beta 1 subunit. Integrin alpha 2/ beta 1 binds collagen and laminin and activates intracellular signaling pathways.

Entrez Gene IDs
3673 (Human); 16398 (Mouse)
Alternate Names
alpha-2 subunit; CD49b antigen; CD49b; Integrin alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha

Product Datasheets

Citation for Mouse Integrin  alpha 2/CD49b Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Indoleamine 2,3-dioxygenase promotes peritoneal dissemination of ovarian cancer through inhibition of natural killercell function and angiogenesis promotion.
    Authors: Nonaka H, Saga Y, Fujiwara H
    Int. J. Oncol., 2011;38(1):113-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Integrin  alpha 2/CD49b Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse Integrin  alpha 2/CD49b Biotinylated Antibody and earn rewards!

Have you used Mouse Integrin  alpha 2/CD49b Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review