Mouse Integrin  alpha 2/CD49b Biotinylated Antibody

  (1 citations)     
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Product Details
Citations (1)
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Integrin alpha 2/CD49b in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Integrin  alpha M is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 4, rmIntegrin alpha 5, and rmIntegrin alpha E is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b
    Tyr27-Thr1129
    Accession # Q62469
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Integrin  alpha 2/CD49b
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse DX5+ splenocytes
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunocytochemistry
Integrin  alpha 2/CD49b in Mouse Splenocytes. Integrin  alpha 2/CD49b was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Integrin  alpha 2/CD49b Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 2/CD49b
The Integrin family proteins are heterodimeric transmembrane receptors comprised of an alpha and a beta subunit. The Integrin alpha 2 subunit is expressed in monocytes, platelets, B, T and NK cells. It forms a non-covalent heterodimer with the Integrin beta 1 subunit. Integrin alpha 2/ beta 1 binds collagen and laminin and activates intracellular signaling pathways.
  • Entrez Gene IDs:
    3673 (Human); 16398 (Mouse)
  • Alternate Names:
    alpha-2 subunit; CD49b antigen; CD49b; Integrin alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Indoleamine 2,3-dioxygenase promotes peritoneal dissemination of ovarian cancer through inhibition of natural killercell function and angiogenesis promotion.
    Authors: Nonaka H, Saga Y, Fujiwara H
    Int. J. Oncol., 2011;38(1):113-20.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Biotinylated Control

Ctrl BAF020 2
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 4
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Streptavidin-Phycoerythrin

Flow F0040 7
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NorthernLights Streptavidin NL557

Flow , IHC , ICC NL999 4
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Streptavidin-Allophycocyanin

Flow F0050 1
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NorthernLights Streptavidin NL637

Flow , IHC , ICC NL998 2
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NorthernLights Streptavidin NL493

Flow , IHC , ICC NL997 2
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 51  
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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